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Abstract:

1. 1. The effect of several metals and reagents on the decarboxylation rate of uroporphyrinogen I by using a 16-fold purified preparation of Uroporphyrinogen Decarboxylase from Rhodopseudomonas palustris, was studied. 2. 2. 1 mM Hg2+ and Cu2+ were strong inhibitors, 1 mM Zn2+ and Fe2+ under certain conditions and 1 mM Fe3+ and Cr3+ also inactivated the enzyme, but Pb2+, Cd2+, and Al3+ did not. Metals inhibition was reversed by 1 mM GSH or CvSH. 3. 3. 0.1 mM DTNB and PCMB, 1 mM pyridoxal phosphate and 100 mM chloral hydrate, as well as 1 mM 2-methoxy-5-nitrotropone and 0.2 mM diethylpyrocarbonate inhibited Uroporphyrinogen Decarboxylase; while GSH, CySH, N-ethylmaleimide, sodium thioglycolate, 1,4-dithioerythritol, EDTA and O-phenantroline did not modify activity. 4. 4. Data obtained would indicate that one cysteine, one or two histidine residues and probably a lysine group are required for enzyme activity. © 1987.

Registro:

Documento: Artículo
Título:Biosynthesis of porphyrins in Rhodopseudomonas palustris-VI. The effect of metals, thiols and other reagents on the activity of uroporphyrinogen decarboxylase
Autor:Koopmann, G.E.; Del C. Batlle, A.M.
Filiación:Ctro. de Investigaciones sobre Porfirinas y Porfirias-CIPYP(CONICET and FCEyN, Univ. of Buenos Aires, Pabellon II, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Palabras clave:4 chloromercuribenzoic acid; 5,5' dithiobis(2 nitrobenzoic acid); Carboxy Lyases; carboxylyase; copper; ferrous ion; mercury; metal; p Chloromercuribenzoic Acid; porphyrin; thiol derivative; uroporphyrinogen decarboxylase; zinc; article; biosynthesis; metabolism; Rhodopseudomonas; Carboxy-Lyases; Chloromercuribenzoates; Copper; Dithionitrobenzoic Acid; Ferrous Compounds; Mercury; Metals; p-Chloromercuribenzoic Acid; Porphyrins; Rhodopseudomonas; Sulfhydryl Compounds; Support, Non-U.S. Gov't; Uroporphyrinogen Decarboxylase; Zinc
Año:1987
Volumen:19
Número:4
Página de inicio:373
Página de fin:377
DOI: http://dx.doi.org/10.1016/0020-711X(87)90011-5
Título revista:International Journal of Biochemistry
Título revista abreviado:Int. J. Biochem.
ISSN:0020711X
CODEN:IJBOB
CAS:4 chloromercuribenzoic acid, 59-85-8; 5,5' dithiobis(2 nitrobenzoic acid), 69-78-3; carboxylyase, 9027-22-9; copper, 15158-11-9, 7440-50-8; ferrous ion, 15438-31-0; mercury, 14302-87-5, 7439-97-6; porphyrin, 24869-67-8; thiol derivative, 13940-21-1; uroporphyrinogen decarboxylase, 9024-70-8; zinc, 7440-66-6; Carboxy-Lyases, EC 4.1.1.; Chloromercuribenzoates; Copper, 7440-50-8; Dithionitrobenzoic Acid, 69-78-3; Ferrous Compounds; Mercury, 7439-97-6; Metals; p-Chloromercuribenzoic Acid, 59-85-8; Porphyrins; Sulfhydryl Compounds; Uroporphyrinogen Decarboxylase, EC 4.1.1.37; Zinc, 7440-66-6
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Referencias:

  • Batlle del, Benson, Rimington, Purification and properties of coproporphyrinogenase (1965) Biochem. J., 97, pp. 731-740
  • Batlle del, Salamanca de, Chinarro, Afonso, Stella, Photodynamic inactivation of red cell uroporphyrinogen decarboxylase by porphyrins (1986) Int. J. Biochem., 18, pp. 143-147
  • Blekkenhorst, Eales, Pimstone, Iron and porphyria cutanea tarda: activation of uroporphyrinogen decarboxylase by ferrous iron in porphyria cutanea tarda (1979) S. Afr. med. J., 56, pp. 918-920
  • De Verneuil, Sassa, Kappas, Purification and properties of uroporphyrinogen decarboxylase from human erythrocytes. A single enzyme catalyzing the four sequential decarboxylations of uroporphyrinogens I and III (1983) J. biol. Chem., 258, pp. 2454-2460
  • Elder, Tovey, Sheppard, Purification of uroporphyrinogen decarboxylase from human erythrocytes. Immunochemical evidence for a single protein with decarboxylase activity in human erythrocytes and liver (1983) Biochem. J., 215, pp. 45-55
  • Jones, Vaughn, HSAB theory and acute metal ion toxicity band detoxification processes (1978) J. Inorg. Nucl. Chem., 40, pp. 2081-2088
  • Kappas, Sassa, Anderson, The porphyrias (1983) The Metabolic Basis of Inherited Disease, pp. 1301-1384. , J.B. Stanbury, J.B. Wyngaarden, D.S. Fredrickson, J.L. Goldstein, M.S. Brown, MacGraw-Hill, New York
  • Kardish, Woods, Measurement of heme biosynthetic enzymes in mammalian tissues. I. Uroporphyrinogen decarboxylase (1980) J. appl. Biochem., 2, pp. 159-167
  • Kawanishi, Seki, Sano, Uroporphyrinogen decarboxylase. Purification, properties and inhibition by polychlorinated biphenyl isomers (1983) J. biol. Chem., 258, pp. 4285-4292
  • Klopman, Metal hardness classification (1968) J. Am. Chem. Sci., 90, pp. 223-230
  • Koopmann, Juknat de Geralnik, Batlle del, Porphyrin biosynthesis in Rhodopseudomonas palustris V: Purification of porphyrinogen decarboxylase and some unusual properties (1986) Int. J. Biochem., 18, pp. 935-944
  • Lehninger, (1975) Biochemistry, p. 78. , 2nd end, Worth, New York
  • Mauzerall, Granick, Porphyrin biosynthesis in erythrocytes III. Uroporphyrinogen and its decarboxylase (1958) J. biol. Chem., 232, pp. 1141-1162
  • Mukerji, Pimstone, Burns, Dual mechanism of inhibition of rat liver uroporphyrinogen decarboxylase activity by ferrous iron: Its potential role in the genesis of porphyria cutanea tarda (1984) Gastroenterology, 87, pp. 1248-1254
  • Mukerji, Pimstone, In vitro studies of the mechanism of inhibition of rat liver uroporphyrinogen decarboxylase activity by ferrous iron under anaerobic conditions (1986) Archs Biochem. Biophys., 244, pp. 619-629
  • Romeo, Levin, Uroporphyrinogen decarboxylase from mouse spleen (1971) Biochim. biophys. Acta, 230, pp. 330-341
  • Seehra, Gore, Chaudhry, Jordan, 5-Aminolevulinic acid dehydratase: The role of sulfhydryl groups in 5-aminolevulinic acid dehydratase from bovine liver (1981) Eur. J. Biochem., 114, pp. 263-269
  • Setlow, Mansour, Studies on heart phosphofructokinase. Nature of the enzyme desensitized to allosteric control by photo-oxidation and by acilation with ethoxyformic anhydride (1970) J. biol. Chem., 245, pp. 5524-5533
  • Smith, Francis, Synergism of iron and hexachlorobenzene inhibits hepatic uroporphyrinogen decarboxylase in inbred mice (1983) Biochem. J., 214, pp. 909-913
  • Straka, Kushner, Purification and characterization of bovine hepatic uroporphyrinogen decarboxylase (1983) Biochemistry, 22, pp. 4664-4672
  • Tamaoki, Murase, Minato, Nakanishi, Reversible chemical modification of protein by 2-methoxy-5-nitrotropone (1967) J. Biochem., Tokyo, 62, pp. 7-14
  • Tomio, García, San Martín de Viale, Grinstein, Porphyrin biosynthesis VII. Porphyrinogen carboxy-lyase from avian erythrocytes. Purification and properties (1970) Biochim. biophys. Acta, 198, pp. 353-363
  • Woods, Kardish, Fowler, Studies on the action of porphyrinogenic trace metals on the activity of hepatic uroporphyrinogen decarboxylase (1981) Biochem. biophys. Res. Commun., 103, pp. 264-271
  • Woods, Eaton, Lukens, Studies on porphyrin metabolism in the kidney. Effects of trace metals and glutathione on renal uroporphyrinogen decarboxylase (1984) Molec. Pharmacol., 26, pp. 336-341

Citas:

---------- APA ----------
Koopmann, G.E. & Del C. Batlle, A.M. (1987) . Biosynthesis of porphyrins in Rhodopseudomonas palustris-VI. The effect of metals, thiols and other reagents on the activity of uroporphyrinogen decarboxylase. International Journal of Biochemistry, 19(4), 373-377.
http://dx.doi.org/10.1016/0020-711X(87)90011-5
---------- CHICAGO ----------
Koopmann, G.E., Del C. Batlle, A.M. "Biosynthesis of porphyrins in Rhodopseudomonas palustris-VI. The effect of metals, thiols and other reagents on the activity of uroporphyrinogen decarboxylase" . International Journal of Biochemistry 19, no. 4 (1987) : 373-377.
http://dx.doi.org/10.1016/0020-711X(87)90011-5
---------- MLA ----------
Koopmann, G.E., Del C. Batlle, A.M. "Biosynthesis of porphyrins in Rhodopseudomonas palustris-VI. The effect of metals, thiols and other reagents on the activity of uroporphyrinogen decarboxylase" . International Journal of Biochemistry, vol. 19, no. 4, 1987, pp. 373-377.
http://dx.doi.org/10.1016/0020-711X(87)90011-5
---------- VANCOUVER ----------
Koopmann, G.E., Del C. Batlle, A.M. Biosynthesis of porphyrins in Rhodopseudomonas palustris-VI. The effect of metals, thiols and other reagents on the activity of uroporphyrinogen decarboxylase. Int. J. Biochem. 1987;19(4):373-377.
http://dx.doi.org/10.1016/0020-711X(87)90011-5