Navegar

Colección

Datos Estadísticas

Artículo

Vazquez, E.S.; Buzaleh, A.M.; Wider, E.A.; del C. Batlle, A.M. "Soluble and immobilized RP. palustris rhodanese-II. Some particular kinetic behaviour" (1987) International Journal of Biochemistry. 19(12):1199-1203
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

1. 1. Kinetic studies were carried out on the soluble and immobilized Rhodanese. 2. 2. The soluble enzyme showed a typical Michaelis-Menten behaviour, an inhibitory effect was observed at high thiosulphate and cyanide concentrations. 3. 3. The product sulphite was also an inhibitor, instead thiocyanate increased the enzyme velocity when it was added to the incubation mixture. 4. 4. A ping-pong mechanism was proposed for Rp. palustris Rhodanese with a stable (free enzyme: E) and an unstable (sulfur substituted enzyme: ES) kinetic enzyme form. 5. 5. The insolubilized Rhodanese presented an unusual kinetic behaviour, with sigmoid shape substrate profiles and non-linear double reciprocal plots. 6. 6. From the empirical Hill equation, positive cooperativity (n>1) was found for both substrates. © 1987.

Registro:

Documento: Artículo
Título:Soluble and immobilized RP. palustris rhodanese-II. Some particular kinetic behaviour
Autor:Vazquez, E.S.; Buzaleh, A.M.; Wider, E.A.; del C. Batlle, A.M.
Filiación:Ctro. de Investigaciones sobre Porfirinas y Porfirias. CIPYP (CONICET and Facultdad de Ciencias Exac, Pabellón II, 1428 Buenos Aires, Argentina
Año:1987
Volumen:19
Número:12
Página de inicio:1199
Página de fin:1203
DOI: http://dx.doi.org/10.1016/0020-711X(87)90103-0
Título revista:International Journal of Biochemistry
Título revista abreviado:Int. J. Biochem.
ISSN:0020711X
CODEN:IJBOB
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v19_n12_p1199_Vazquez

Referencias:

  • Cleland, Steady state kinetics (1970) The Enzymes, 1, pp. 1-65. , P.D. Boyer, Academic Press, New York
  • Cuatrecasas, Protein purification by affinity (1970) J. biol. Chem., 245, pp. 3059-3097
  • De Toma, Westley, Active site peptides of Rhodanese (1970) Biochimica et Biophysica Acta (BBA) - Protein Structure, 207, pp. 144-149
  • Horowitz, Westley, Some molecular properties of Rhodanese (1970) J. biol. Chem., 245, pp. 986-990
  • Jarabak, Westley, Human liver Rhodanese. Non linear kinetic behaviour in a double displacement mechanism (1974) Biochemistry, 13, pp. 3233-3236
  • Leininger, Westley, The mechanism of the Rhodanese-catalyzed thiosulphate-cyanide reaction (1968) J. biol. Chem., 243, pp. 1892-1899
  • Lowry, Rosebrough, Farr, Randall, Protein measurement with the Folin-Phenol reagent (1951) J. biol. Chem., 193, pp. 265-275
  • Mintel, Westley, The Rhodanese reaction mechanisms of sulphur-sulphur bond cleavage of thiosulfonate sulfur (1966) J. biol. Chem., 241, pp. 3381-3385
  • Mintel, Westley, The Rhodanese reaction. Mechanism of thio-sulfate binding (1966) J. biol. Chem., 241, pp. 3386-3389
  • Oi, Purification and some properties of Rhodanese of Trametes sanguinea (1973) Agr. Biol. Chem., 37, pp. 629-633
  • Sörbo, Rhodanese (1955) Methods in Enzymology, 2, pp. 334-338. , S.P. Colowick, N.O. Kaplan, Academic Press, New York
  • van Rensburg, Shabort, Rhodanese from Cercopithecus aethiops (Vervet monkey) liver II. Aspects of enzyme kinetics and mechanisms of action (1984) Int. J. Biochem., 16, pp. 547-551
  • Vázquez, Buzaleh, Wider, Batlle, Soluble and immobilized Rp. palustris Rhodanese I. Optimal conditions (1986) Biotech. appl. Biochem., , In press
  • Villarejo, Westley, Mechanisms of Rhodanese catalysis of thiosulphate-lipoate oxidation-reduction (1963) J. biol. Chem., 238, pp. 4016-4020
  • Villarejo, Westley, Sulfur metabolism of Bacillus subtilis (1966) Biochim. biophys. Acta, 117, pp. 209-216
  • Volini, Alexander, Multiple forms and multiple functions of the Rhodanese (1981) Cyanide in Biology, pp. 77-91. , B. Wennesland, E.E. Conn, C.J. Knowles, J. Westley, F. Wissing, Academic Press, London
  • Volini, Van Sweringen, Chen, Effects of metal-iron binding on Rhodanese activity (1978) Archs. Biochem. Biophys., 191, pp. 205-215
  • Wang, Volini, Studies on the active site of Rhodanese (1968) J. biol. Chem., 243, pp. 5465-6470
  • Wang, Volini, The interdependence of substrate and protein transformations in Rhodanese catalysis (1973) J. biol. Chem., 248, pp. 7376-7385
  • Weng, Russell, Heinrikson, The covalent structure of bovine liver Rhodanese. NH2-terminal sequence and partial structural analysis of tryptic peptides from the citraconylated protein (1978) J. biol. Chem., 253, pp. 8093-8101
  • West, Gaeke, Fixation of sulfur dioxide as disulfitomercurate (II) and subsequent colorimetric estimation (1956) Analyt. Chem., 28, pp. 1816-1819
  • Westley, Rhodanese (1973) Advances in Enzymology, 39, pp. 327-368. , A. Meister, Interscience, New York
  • Westley, Heyse, Mechanisms of sulfur transfer catalysis. Sulphydryl-catalyzed transfer (1971) J. biol. Chem., 246, pp. 1468-1474

Citas:

---------- APA ----------
Vazquez, E.S., Buzaleh, A.M., Wider, E.A. & del C. Batlle, A.M. (1987) . Soluble and immobilized RP. palustris rhodanese-II. Some particular kinetic behaviour. International Journal of Biochemistry, 19(12), 1199-1203.
http://dx.doi.org/10.1016/0020-711X(87)90103-0
---------- CHICAGO ----------
Vazquez, E.S., Buzaleh, A.M., Wider, E.A., del C. Batlle, A.M. "Soluble and immobilized RP. palustris rhodanese-II. Some particular kinetic behaviour" . International Journal of Biochemistry 19, no. 12 (1987) : 1199-1203.
http://dx.doi.org/10.1016/0020-711X(87)90103-0
---------- MLA ----------
Vazquez, E.S., Buzaleh, A.M., Wider, E.A., del C. Batlle, A.M. "Soluble and immobilized RP. palustris rhodanese-II. Some particular kinetic behaviour" . International Journal of Biochemistry, vol. 19, no. 12, 1987, pp. 1199-1203.
http://dx.doi.org/10.1016/0020-711X(87)90103-0
---------- VANCOUVER ----------
Vazquez, E.S., Buzaleh, A.M., Wider, E.A., del C. Batlle, A.M. Soluble and immobilized RP. palustris rhodanese-II. Some particular kinetic behaviour. Int. J. Biochem. 1987;19(12):1199-1203.
http://dx.doi.org/10.1016/0020-711X(87)90103-0