Navegar

Colección

Datos Estadísticas

Artículo

Este artículo esta disponible en la web de forma gratuita y puede ser descargado en su versión final
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

1. 1. Uroporphyrinogen decarboxylase (EC 4.1.1.37) has been purified 16-fold from Rp. palustris to a specific activity of 210 nmol of total decarboxylated porphyrinogens III formed/hr per mg of protein and about 50% yield. The Rp. palustris enzyme exhibits some unusual properties as compared with URO-D from other sources. 2. 2. The purified enzyme is a monomer with a molecular weight of ∼46,000, an isoelectric point of 4.6 and an optimum pH of 6.9 and 6.8 with urogen III and I substrate. Neither GSH nor EDTA seem to be necessary for activity, and the decarboxylation rate and the distribution of the reaction products was not affected either by the presence or absence of oxygen. 3. 3. The Rp. palustris enzyme is a thermo-stable protein, heating at 60°C for 15 min enhanced several times activity. This is the first time that the heat treatment is included as one of the steps to purify URO-D. 4. 4. Thermal activation followed an identical profile using either substrate. The ratios of specific activity for the type III and I isomer of urogen remained constant throughout the purification. These findings are indicating that a single enzyme catalyzes the four decarboxylations occurring from urogen to coprogen. 5. 5. Kinetic data employing urogen III and I as substrate showed that the pattern of accumulated intermediates was rather different depending on whether type III or I isomer was used. 6. 6. While decarboxylation of urogen III responds to the usual scheme: {A figure is presented} where v1≫v2 and decarboxylation of heptagen III is the rate-controlling step. 7. 7. Decarboxylation of urogen I revealed a completely different and characteristic picture fitting the scheme: {A figure is presented} where again v′1≫v′2 and the removal of the final carboxyl group from pentagen I becomes the rate-limiting step. © 1986.

Registro:

Documento: Artículo
Título:Porphyrin biosynthesis in rhodopseudomonas palustris-V. Purification of porphyrinogen decarboxylase and some unusual properties
Autor:Koopmann, G.E.; Juknat de Geralnik, A.A.; del C. Batlle, A.M.
Filiación:Centro de Investigaciones sobre Porfirinas y Porfirias-CIPYP-(CONICET-FCEN, UBA), Ciudad Universitaria, 1428 Buenos Aires, Argentina
Año:1986
Volumen:18
Número:10
Página de inicio:935
Página de fin:944
DOI: http://dx.doi.org/10.1016/0020-711X(86)90075-3
Título revista:International Journal of Biochemistry
Título revista abreviado:Int. J. Biochem.
ISSN:0020711X
CODEN:IJBOB
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_0020711X_v18_n10_p935_Koopmann.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v18_n10_p935_Koopmann

Referencias:

  • Barnard, Akhtar, Stereochemistry of porphyrinogen carboxylase reaction in haem biosynthesis (1975) Journal of the Chemical Society, Chemical Communications, pp. 494-496
  • Batlle, Estimation of molecular weights of proteins by Bio-gel P gel filtration (1967) Journal of Chromatography A, 8, pp. 82-88
  • Batlle, Grinstein, Studies on the nature of a biosynthetic porphyrin and its identification with the so called “208” porphyrin (1962) Biochimica et Biophysica Acta, 57, pp. 191-194
  • Batlle, Grinstein, Porphyrin biosynthesis Phyriaporphyrinogen III a normal intermediate in the biosynthesis of protoporphyrin 9 (1962) Biochimica et Biophysica Acta, 62, pp. 197-200
  • Batlle, Grinstein, Porphyrin biosynthesis Studies on erythrocyte preparations (1964) Biochimica et Biophysica Acta (BBA) - General Subjects, 82, pp. 1-12
  • Batlle, Grinstein, Porphyrin biosynthesis II. Phyriaporphyrinogen III, a normal intermediate in the biosynthesis of haem (1964) Biochim. biophys. Acta, 82, pp. 13-20
  • Batlle, Stella, Delta amino-laevulinate dehydratase: its mechanism of action (1978) Int. J. Biochem., 9, pp. 861-864
  • Batlle, Koopmann, The enzymic decarboxylation of porphyrinogens Properties and mechanism of action Review (1986) Int J Biochem, , In preparation
  • Batlle, Benson, Rimington, Purification and properties of coproporphyrinogenase (1965) Biochem J, 97, pp. 731-740
  • Batlle, Stella, Ferramola, Sopena, Wider, Sancovich, Porphyrin biosynthesis. Immobilized enzymes and ligands X. A novel approach to the study of the relationship between the quarternary structure of aminolevulinate dehydratase and its activity (1978) Int. J. Biochem., 9, pp. 401-406
  • Bodman, (1960) Chromatographic and Electrophoretic Techniques, 2, p. 129. , I. Smith, William Hernemann, London
  • Bogorad, The enzymatic synthesis of porphyrins from porfobilinogen. III. Uroporphyrinogens as intermediates (1958) J. biol. Chem., 233, pp. 516-519
  • Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Analyt. Biochem., 72, pp. 248-254
  • Cantoni, dal Fiume, Ruggieri, Decarboxylation of uroporphyrinogen I and III in 2,3,7,8-tetrachlorodibenzo-p-dioxin induced porphyria in mice (1984) Int. J. Biochem., 16, pp. 561-565
  • de Matteis, Prior, Rimington, Nervous and biochemical disturbances following hexachlorobenzene intoxication (1961) Nature (Lond.), 191, pp. 363-366
  • de Verneuil, Grandchamp, Nordmann, Some kinetic properties of human red cell uroporphyrinogen decarboxylase (1980) Biochim. biophys. Acta, 611, pp. 174-186
  • de Verneuil, Sassa, Kappas, Purification and properties of uroporphyrinogen decarboxylase from human erythrocytes. A single enzyme catalyzing the four sequential decarboxylations of uroporphyrinogens I and III (1983) J. biol. Chem., 258, pp. 2454-2460
  • Elder, Tovey, Uroporphyrinogen decarboxylase activity of human tissues (1977) Biochem. Soc. Trans., 5, pp. 1470-1472
  • Elder, Urquhart, Human uroporphyrinogen decarboxylase. Do tissue-specific isoenzymes exist? (1984) Biochem. Soc. Trans., 12, pp. 663-664
  • Elder, Tovey, Sheppard, Purification of uroporphyrinogen decarboxylase from human erythrocytes. Immunochemical evidence for a single protein with decarboxylating activity in human erythrocytes and liver (1983) Biochem. J., 215, pp. 45-55
  • Garcia, San Martin de Viale, Tomio, Grinstein, Porphyrin biosynthesis X. Porphyrinogen carboxy-lyase from avian erythrocytes further properties (1973) Biochim. biophys. Acta, 309, pp. 203-210
  • Hoare, Heath, Intermediates in the biosynthesis of porphyrins from porphobilinogen by Rhodopseudomonas spheroides (1958) Nature, 181, pp. 1592-1593
  • Hoare, Heath, The biosynthesis of porphyrins from porphobilinogen by Rhodopseudomonas spheroides 2. The partial purification and some properties of porphobilinogen deaminase and uroporphyrinogen decarboxylase (1959) Biochem. J., 73, pp. 679-690
  • Jackson, Ferramola, Sancovich, Evans, Matlin, Ryder, Smith, Hepta and hexa-carboxylic porphyrinogen intermediates in the haem biosynthesis (1976) Ann. clin. Res., 8, pp. 64-69
  • Jackson, Sancovich, Ferramola, Evans, Games, Matlin, Elder, Smith, Macrocyclic intermediates in the biosynthesis of porphyrins (1976) Phil. Trans. R. Soc. Lond. B, 273, pp. 191-206
  • Jackson, Nagaraja Rao, Supphayen, Intermediates between uroporphyrinogen I and coproporphyrinogen I (1977) Journal of the Chemical Society, Chemical Communications, pp. 696-698
  • Juknat, Kotler, Batlle, (1986) Porphyrin biosynthesis in Rhodopseudomas spheroides VII: Enzymic cyclotetramerization of porphobilinogen into uroporphyrinogens, , In preparation
  • Kardish, Woods, Measurement of heme biosynthetic enzymes in mammalian tissues (1980) J. appl. Biochem., 2, pp. 159-167
  • Kawanishi, Seki, Sano, Uroporphyrinogen decarboxylase. Purification, properties and inhibition by polychlorinated biphenyl isomers (1983) J. biol. Chem., 258, pp. 4285-4292
  • Koopmann, Juknat, Batlle, Porphyrin biosynthesis in Rhodopseudomas palustris VI: Evidence for aerobic and anaerobic coproporphyrinogenase activities (1986) Comp. Biochem. Physiol., , Part B. In preparation
  • Kotler, Juknat, Batlle, (1986) Porphyrin biosynthesis in Rhodopseudomonas palustris VIII: Studies on porphobilinogen deaminase, , In preparation
  • Kushner, Barbuto, Lee, An inherited enzymatic defect in porphyria cutanea tarda: decreased uroporphyrinogen decarboxylase activity (1976) J. clin. Invest., 58, pp. 1089-1097
  • Locascio, Tigier, Batlle, Estimation of molecular weight of proteins by agarose filtration (1969) J. Chromatogr., 40, pp. 453-457
  • Lombardo, Purificación, propiedades y papel de la Porfobilinógeno sintetasa en Rhodopseudomonas palustris (1986) Ph. D. Thesis University of Buenos Aires
  • Llambias, Batlle, Studies on the porphobilinogen deaminase-uroporphyrinogen cosynthetase systsm of cultured soya-bean cells (1971) Biochem J, 121, pp. 327-340
  • Llambias, Batlle, Porphyrin biosynthesis VIII Avian erythrocyte porphobilinogen deaminase-uroporphyrinogen III cosynthetase Its purification properties and separation of its components (1971) Biochimica et Biophysica Acta (BBA) - Enzymology, 227, pp. 180-191
  • Mauzerall, Granick, Porphyrin biosynthesis in erythrocytes III. Uroporphyrinogen and its decarboxylase (1958) J. biol. Chem., 232, pp. 1141-1162
  • Nicholas, Rimington, Isolation of uroporphyrin III. A further study of Turacin (1951) Biochem. J., 50, pp. 194-201
  • Polo, Stella, Wider, Batlle, Lenczner, Altuna, Magnin, Porfirinas en la piel de porfiria cutánea tardía por cromatografiá líquida a alta presion (1983) Prens. Med. Arg., 70 (11), pp. 433-440
  • Romeo, Levin, Uroporphyrinogen decarboxylase from mouse spleen (1971) Biochim. biophys. Acta, 230, pp. 330-341
  • Rossetti, Juknat de Geralnik, Kotler, Fumagalli, Batlle, Occurrence of multiple molecular forms of porphobilinogenase in diverse organisms: The minimum quaternary structure of porphobilinogenase is a promoter of one deaminase and one isomerase domain (1980) Int. J. Biochem., 12, pp. 761-767
  • Rytka, Bilinski, Labbe-Bois, Modified uroporphyrinogen decarboxylase activity in a yeast mutant which mimics prophyria cutanea tarda (1984) Biochem. J., 218, pp. 405-413
  • Sancovich, Batlle, Grinstein, Porphyrin biosynthesis VI. Separation and purification of porphobilinogen deaminase and uroporphyrinogen isomerase from cow liver. Porphobilinogenase, an allosteric enzyme (1969) Biochim. biophys. Acta, 191, pp. 130-143
  • San Martin de Viale, Grinstein, Porphyrin biosynthesis IV 5- and 6-COOH porphyrinogens (Type III) as normal intermediates in haem biosynthesis (1968) Biochimica et Biophysica Acta (BBA) - General Subjects, 158, pp. 79-91
  • San Martin de Viale, Garcia, Pisarev, Tomio, Grinstein, Studies on uroporphyrinogen-decarboxylase from chicken erythrocytes (1969) FEBS Lett., 5, pp. 149-152
  • Schoua, Caracterización de la Succinil CoA sintetasa de Rhodopseudomonas palustris (1986) Ph. D. Thesis University of Buenos Aires
  • Seubert, Seubert, Eine neue, schnelle Methode zur Trennung von Porphyrinen mittels HPTLC (high-performance thin-layer chromatography) (1979) Deut. Med. Wochenschr., 104, p. 1469
  • Smith, Francis, Decarboxylation of porphyrinogens by rat liver uroporphyrinogen decarboxylase (1979) Biochem. J., 183, pp. 455-458
  • Smith, Ferramola, Sancovich, Evans, Mattin, Ryder, Jackson, Pentacarboxylic intermediates in haem biosynthesis (1976) Ann. clin. Res., 8, pp. 89-91
  • Stafforini, Polo, Stella, Wider de Xifra, Batlle, Pig liver aminolevulineate dehydratase II. Studies on different conditions influencing its molecular weight determination (1980) Int. J. Biochem., 12, pp. 757-760
  • Straka, Kushner, Purification and characterization of bovine hepatic uroporphyrinogen decarboxylase (1983) Biochemistry, 22, pp. 4664-4672
  • Taljaard, Shanley, Joubert, Decreased uroporphyrinogen decarboxylase activity in experimental symptomatic porphyria (1971) Life Sci., 10, pp. 887-893
  • Tomio, Garcia, San Martin de Viale, Grinstein, Porphyrin biosynthesis VII. Porphyrinogen carboxy-lyase from avian erythrocytes. Purification and properties (1970) Biochim. biophys. Acta, 198, pp. 353-363
  • Urban-Grimal, Labbe-Bois, Genetic and biochemical characterization of mutants of Saccharomyces cerevisiae blocked in six different steps of heme biosynthesis (1981) Molec. Gen. Genet., 183, pp. 85-92
  • Vazquez, Buzaleh, Wider, Batlle, (1986) Porphyrin biosynthesis in Rhodopseudomonas palustris IX. Purification and kinetics of rhodanese, , In preparation
  • Viale, Wider, Batlle, Porphyrin biosynthesis in Rhodopseudomonas palustris II. Evidence on the existence of a factor regulating aminoleuvulinate synthetase activity (1980) Int. J. Biochem., 12, pp. 729-733
  • Viale, Lorenti, Wider, Batlle, Biosíntesis de bacterioclorofila en Rhodopseudomonas palustris (1980) Rev. Arg. Microb., 12 (1), pp. 1-9
  • Viale, Wider, Batlle, Algunas propiedades de la δ-aminolevulinato sintetasa de Rhodopseudomonas palustris (1983) Rev. Arg. Microb., 15 (4), pp. 233-238
  • Viale, Wider, Batlle, Adaptación de Rhodopseudomonas palustris a cambios de condiciones atmosféricas (1985) Rev. Arg. Microb., 17 (2), pp. 75-79
  • With, Uroporphyrin from Turacin: a simplified method (1957) Nature (Lond.), 179, p. 824

Citas:

---------- APA ----------
Koopmann, G.E., Juknat de Geralnik, A.A. & del C. Batlle, A.M. (1986) . Porphyrin biosynthesis in rhodopseudomonas palustris-V. Purification of porphyrinogen decarboxylase and some unusual properties. International Journal of Biochemistry, 18(10), 935-944.
http://dx.doi.org/10.1016/0020-711X(86)90075-3
---------- CHICAGO ----------
Koopmann, G.E., Juknat de Geralnik, A.A., del C. Batlle, A.M. "Porphyrin biosynthesis in rhodopseudomonas palustris-V. Purification of porphyrinogen decarboxylase and some unusual properties" . International Journal of Biochemistry 18, no. 10 (1986) : 935-944.
http://dx.doi.org/10.1016/0020-711X(86)90075-3
---------- MLA ----------
Koopmann, G.E., Juknat de Geralnik, A.A., del C. Batlle, A.M. "Porphyrin biosynthesis in rhodopseudomonas palustris-V. Purification of porphyrinogen decarboxylase and some unusual properties" . International Journal of Biochemistry, vol. 18, no. 10, 1986, pp. 935-944.
http://dx.doi.org/10.1016/0020-711X(86)90075-3
---------- VANCOUVER ----------
Koopmann, G.E., Juknat de Geralnik, A.A., del C. Batlle, A.M. Porphyrin biosynthesis in rhodopseudomonas palustris-V. Purification of porphyrinogen decarboxylase and some unusual properties. Int. J. Biochem. 1986;18(10):935-944.
http://dx.doi.org/10.1016/0020-711X(86)90075-3