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Abstract:

1. 1. The activity of the following enzymes involved in the biosynthesis of porphyrins was determined in endosymbiote-free and endosymbiote-containing Crithidia deanei grown in a chemically defined medium: succinyl Coenzyme A synthetase (Suc.CoA-S), 5-aminolevulinate synthetase (ALA-S), 4,5-dioxovaleric acid transaminase (DOVA-T), 5-aminolevulinate dehydratase (ALA-D), por- phobilinogenase (PBGase), deaminase and heme synthetase (Heme-S). The amount of 5-aminolevulinic acid (ALA) and porphobilinogen, porphyrins and heme was also determined. 2. 2. ALA and PBG were detected in C. deanei. The levels of free porphyrins was low. Heme concentration was nil. 3. 3. The activity of ALA-D. deaminase and PBGase was not detected in C deanei. 4. 4. The activity of Suc.CoA-S and ALA-S were twice higher in symbiote-containing than in aposymbiotic C. deanei. Aposymbiotic cells had a higher activity of DOVA-T than symbiote-containing cells. 5. 5. The level of Heme-S, measured using protoporphyrin as substrate, was twice as high in symbiotecontaining than in symbiote-free cells,. © 1985.

Registro:

Documento: Artículo
Título:Heme synthesis in Crithidia deanei: Influence of the endosymbiote
Autor:Salzman, T.A.; Del Batlle, C.A.M.; Angluster, J.; De Souza, W.
Filiación:Centro de Investigaciones sobre Porfirinas y Porfirias-CIPyP. FCEyN, Universidad de Buenos Aires, Ciudad Universitaria. Pabellon II, 1428 Buenos Aires, Argentina
Instituto de Microbiologia, UFRJ, Ilha do Fundão, Rio de Janeiro, Brazil
Instituto de Biofisica. UFRJ, Ilha do Fundao, 21941 Rio de Janeiro, Brazil
Palabras clave:enzyme; heme; crithidia deanei; nonhuman; protozoon; 5-Aminolevulinate Synthetase; Aminolevulinic Acid; Comparative Study; Crithidia; Heme; Porphyrins; Rickettsiaceae; Succinate-CoA Ligases; Support, Non-U.S. Gov't; Symbiosis
Año:1985
Volumen:17
Número:12
Página de inicio:1343
Página de fin:1347
DOI: http://dx.doi.org/10.1016/0020-711X(85)90058-8
Título revista:International Journal of Biochemistry
Título revista abreviado:Int. J. Biochem.
ISSN:0020711X
CODEN:IJBOB
CAS:heme, 14875-96-8; 5-Aminolevulinate Synthetase, EC 2.3.1.37; Aminolevulinic Acid, 106-60-5; Heme, 14875-96-8; Porphyrins; Succinate-CoA Ligases, EC 6.2.1.-
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_0020711X_v17_n12_p1343_Salzman.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v17_n12_p1343_Salzman

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Citas:

---------- APA ----------
Salzman, T.A., Del Batlle, C.A.M., Angluster, J. & De Souza, W. (1985) . Heme synthesis in Crithidia deanei: Influence of the endosymbiote. International Journal of Biochemistry, 17(12), 1343-1347.
http://dx.doi.org/10.1016/0020-711X(85)90058-8
---------- CHICAGO ----------
Salzman, T.A., Del Batlle, C.A.M., Angluster, J., De Souza, W. "Heme synthesis in Crithidia deanei: Influence of the endosymbiote" . International Journal of Biochemistry 17, no. 12 (1985) : 1343-1347.
http://dx.doi.org/10.1016/0020-711X(85)90058-8
---------- MLA ----------
Salzman, T.A., Del Batlle, C.A.M., Angluster, J., De Souza, W. "Heme synthesis in Crithidia deanei: Influence of the endosymbiote" . International Journal of Biochemistry, vol. 17, no. 12, 1985, pp. 1343-1347.
http://dx.doi.org/10.1016/0020-711X(85)90058-8
---------- VANCOUVER ----------
Salzman, T.A., Del Batlle, C.A.M., Angluster, J., De Souza, W. Heme synthesis in Crithidia deanei: Influence of the endosymbiote. Int. J. Biochem. 1985;17(12):1343-1347.
http://dx.doi.org/10.1016/0020-711X(85)90058-8