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Abstract:

1. 1. No changes in ALA-S activity were observed when different preparations of R. palustris were stored at 4°C for various periods of time. 2. 2. Mixing supernatants from pigmented and decoloured R. palustris cells, showed that the activity of ALA-S was several times higher than expected, suggesting the presence of an activator. 3. 3. Supernatants from photosynthetically and aerobically grown cells were heated and the effect of the protein-free supernatant was tested on both red and white supernatants. The heated supernatant from aerobic cells increased ALA-S when added to red and white preparations, but the heated red supernatant only activated red supernatant and had no action on the white cells enzyme. 4. 4. By gel filtration on Sephadex G-25 of cell free extracts from R. palustris either aerobically or anaerobically grown, a low molecular weight compound was separated, which added back to the homologeous enzyme enhanced its activity confirming the existence of one or two low-molecular weight and heat-stable factors which would act stimulating ALA-S activity. 5. 5. A scheme is proposed to explain the role of these factors on the control of ALA-S in R. palustris. © 1980.

Registro:

Documento: Artículo
Título:Porphyrin biosynthesis in rhodopseudomonas palustris-II. Evidence on the existence of a factor regulating aminolevulinate synthetase activity
Autor:Viale, A.A.; De Xifra, E.A.W.; Del C. Batlle, A.M.
Filiación:Centro de Investigaciones sobre Porfirinas y Porfirias-CIPYP, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon II, 4o Piso, 1428 BA, Argentina
Palabras clave:5 aminolevulinate synthase; porphyrin; aerobic metabolism; article; biosynthesis; kinetics; metabolism; molecular weight; photosynthesis; Rhodopseudomonas; 5-Aminolevulinate Synthetase; Aerobiosis; Kinetics; Molecular Weight; Photosynthesis; Porphyrins; Rhodopseudomonas; Support, Non-U.S. Gov't
Año:1980
Volumen:12
Número:5-6
Página de inicio:729
Página de fin:733
DOI: http://dx.doi.org/10.1016/0020-711X(80)90153-6
Título revista:International Journal of Biochemistry
Título revista abreviado:Int. J. Biochem.
ISSN:0020711X
CODEN:IJBOB
CAS:5 aminolevulinate synthase, 9037-14-3; porphyrin, 24869-67-8; 5-Aminolevulinate Synthetase, EC 2.3.1.37; Porphyrins
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_0020711X_v12_n5-6_p729_Viale.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v12_n5-6_p729_Viale

Referencias:

  • Del Batlle, Benson, Rimington, Purification and properties of coproporphyrinogenase (1965) Biochem J, 97, pp. 731-740
  • Jordan, Shemin, Ä-Aminolevulinic acid synthetase (1972) The Enzyme, 7, pp. 339-356. , Academic Press, New York
  • Kikuchi, Kumar, Talmage, Shemin, The enzymatic synthesis of delta-aminolevulinic acid (1958) J. hiol. Chem., 233, pp. 1214-1219
  • Kikuchi, Shemin, Bachman, The enzymic synthesis of Ä-aminilevulinic acid (1958) Biochem. biophys. Acta, 28, pp. 219-229
  • Lascelles, Adaptation to form bacteriochlorophyll in R. spherondes: Changes in activity of enzymes concerned in pyrrole synthesis (1959) Biochem. J., 72, pp. 508-518
  • Marriot, Neuberger, Tait, Control of aminolaevulinate synthetase activity in R. spheroides (1969) Biochem. J., 111, pp. 385-394
  • Neuberger, Sandy, Tait, Control of 5-aminolaevulinate synthetase activity in R. spheroides (1973) Biochem. J., 136, pp. 477-490
  • Tuboi, Hayasaka, Control of < 5-aminolevulinate synthetase activity in R. spheroides (1972) Archs Biochem. Biophys., 150, pp. 690-697
  • Tuboi, Kim, Kikuchi, Occurrence of a specific and reversible inhibitor of < 5-aminolevulinate synthetase in extracts of R. spheroides (1969) Archs Biochem. Biophys., 130, pp. 92-100
  • Viale, Wider De Xifra, Batlle A.M. Del, Porphyrin biosynthesis in Rhodopseudomonas palustris I (1980) Rev. Arg. Microb., , In press
  • Wider De Xifra, Batlle A.M. Del, Tigier, 5-aminolevulinate synthetase in extracts of cultured soybean cells (1971) Biochimica et Biophysica Acta (BBA) - Enzymology, 235, pp. 511-517
  • Wider De Xifra, Sandy, Da Vies, Neuberger, Control of 5-aminolevulinate synthetase activity in R. spheroides (1976) Phil. Trans. R. Soc. Lond. B, 273, pp. 79-98

Citas:

---------- APA ----------
Viale, A.A., De Xifra, E.A.W. & Del C. Batlle, A.M. (1980) . Porphyrin biosynthesis in rhodopseudomonas palustris-II. Evidence on the existence of a factor regulating aminolevulinate synthetase activity. International Journal of Biochemistry, 12(5-6), 729-733.
http://dx.doi.org/10.1016/0020-711X(80)90153-6
---------- CHICAGO ----------
Viale, A.A., De Xifra, E.A.W., Del C. Batlle, A.M. "Porphyrin biosynthesis in rhodopseudomonas palustris-II. Evidence on the existence of a factor regulating aminolevulinate synthetase activity" . International Journal of Biochemistry 12, no. 5-6 (1980) : 729-733.
http://dx.doi.org/10.1016/0020-711X(80)90153-6
---------- MLA ----------
Viale, A.A., De Xifra, E.A.W., Del C. Batlle, A.M. "Porphyrin biosynthesis in rhodopseudomonas palustris-II. Evidence on the existence of a factor regulating aminolevulinate synthetase activity" . International Journal of Biochemistry, vol. 12, no. 5-6, 1980, pp. 729-733.
http://dx.doi.org/10.1016/0020-711X(80)90153-6
---------- VANCOUVER ----------
Viale, A.A., De Xifra, E.A.W., Del C. Batlle, A.M. Porphyrin biosynthesis in rhodopseudomonas palustris-II. Evidence on the existence of a factor regulating aminolevulinate synthetase activity. Int. J. Biochem. 1980;12(5-6):729-733.
http://dx.doi.org/10.1016/0020-711X(80)90153-6