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Abstract:

1. 1. The effect on the activity of soybean callus Succinyl CoA Synthetase of EDTA, 8-OH quinoline, o-phenantroline, α,α $ ́dipyridyl, sodium azide and sodium cyanide was assayed. EDTA completely inhibit the enzyme, cyanide only diminished its activity 30%, and the other compounds significantly enhanced Succinyl CoA Synthetase activity. 2. 2. The action of various divalent cation chlorides was studied. The enzyme activation capacities of Mn2+ and Mg2+ were rather similar at all concentrations tested. At 4 and 5 mM Co2+ activates the enzyme more than Mg2+ and Mn2+. Complete inactivation was produced by Pb2+and Hg2+ at concentrations as low as 1 mM. Ca2+, Zn2+, Fe2+ and Cd2+ could not replace Mg2+ either, instead they inhibited Succinyl CoA Synthetase. 3. 3. The assay with the monovalent cations chlorides K +, NH+ 4, Na+ and Li+ showed that maximum activation for the first three was found at 50-60 mM while Li+ had no effect. Increasing concentrations of these cations progressivey progressively inhibited enzyme activity. © 1980.

Registro:

Documento: Artículo
Título:Porphyrin biosynthesis in the soybean callus system-XVII. Effect of monovalent and divalent cations on the activity of succinyl CoA synthetase
Autor:De Xifra, E.A.W.; Del C. Batlle, A.M.
Filiación:Centro de Investigaciones sobre Porfirinas y Porfirias-CIPYP, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon II, 4to Piso, Nuiriez, 1428 Buenos Aires, Argentina
Palabras clave:magnesium; manganese; porphyrin; succinyl coenzyme a synthetase; animal experiment; higher plant; Cations, Divalent; Cations, Monovalent; Coenzyme A Ligases; Kinetics; Plants; Porphyrins; Soybeans; Succinate-CoA Ligases; Support, Non-U.S. Gov't
Año:1980
Volumen:12
Número:5-6
Página de inicio:717
Página de fin:719
DOI: http://dx.doi.org/10.1016/0020-711X(80)90150-0
Título revista:International Journal of Biochemistry
Título revista abreviado:Int. J. Biochem.
ISSN:0020711X
CODEN:IJBOB
CAS:magnesium, 7439-95-4; manganese, 16397-91-4, 7439-96-5; porphyrin, 24869-67-8; succinyl coenzyme A synthetase, 37341-57-4, 9014-36-2, 9080-33-5; Cations, Divalent; Cations, Monovalent; Coenzyme A Ligases, EC 6.2.1.; Porphyrins; Succinate-CoA Ligases, EC 6.2.1.-
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v12_n5-6_p717_DeXifra

Referencias:

  • Batlle A.M. Del, Llambías, Wider De Xifra, Tigier, Porphyrin biosynthesis in the soybean callus tissue system—XV. The effect of growth conditions (1975) Int. J. Biochem., 6, pp. 591-606
  • Bush, Influence of certain cations on activity of Succinyl CoA Synthetase from tobacco (1969) PLANT PHYSIOLOGY, 44, pp. 347-350
  • Gibson, Upper, Gunsalus, Succinyl coenzyme A synthetase from Escherichia coll I. Purification and properties (1967) J. biol. Chem., 242, pp. 2474-2477
  • Kaufman, Alivisatos, Purification and properties of the phosphorylating enzyme from spinach (1955) J. biol. Chem., 216, pp. 141-152
  • Labbe, Kurumada, Onisawa, The role of Succinyl CoA Synthetase in the control of haem biosynthesis (1965) Biochimica et Biophysica Acta (BBA) - General Subjects, 3, pp. 403-410
  • Nandi, Waygood, Biosynthesis of porphyrins in wheat leaves I. Succinate:CoA Ligase (ADP) (1965) Can. J. Biochem., 43, pp. 1605-1614
  • Palmer, Wedding, Purification and properties of Succinyl CoA Synthetase from Jerusalem Artichoke mitochondria (1966) Biochim. biophys. Acta, 113, pp. 167-174
  • Stobart, Pinfield, Light-induced changes in Succinyl CoA Synthetase in Barley Seedlings (1970) New Phytologist, 69, pp. 31-35
  • Wider, Del Batlle, Mechanism of action of Succinyl CoA Synthetase. The role of CoA (1973) Enzyme, 16, pp. 128-137
  • Wider, Del Batlle, Thiol groups of soybean callus Succinyl CoA Synthetase (1974) Int. J. Biochem., 5, pp. 129-135
  • Wider, Del Batlle, Studies on soybean callus Succinyl CoA Synthetase. Reaction Mechanism (1976) Porphyrin in Human Diseases, pp. 25-34. , M. Doss, Karger, Basel
  • Wider, Del Batlle, Porphyrin biosynthesis Immobilized enzymes and ligands VI—Studies on Succinyl CoA Synthetase from cultured soybean cells (1977) Biochimica et Biophysica Acta (BBA) - Enzymology, 523, pp. 245-249
  • Wider, Tigier, Properties and regulatory effect on tetrapyrrole biosynthesis of Succinyl CoA Synthetase isolated from soybean callus tissue system (1970) FEBS Lett., 9, pp. 30-32
  • Wider, Tigier, Porphyrin biosynthesis in soybean callus tissue system VIII—Isolation, purification and general properties of Succinyl CoA Synthetase (1971) Enzymologia, 41, pp. 217-231
  • Wider, Mendiara, Batlle A.M. Del, Studies on the reaction mechanism of soybean callus succinyl CoA Synthetase. Phosphorylation of the enzyme on immobilized adenosintriphosphate and enzyme attached to a solid support (1972) FEBS Lett., 37, pp. 275-278

Citas:

---------- APA ----------
De Xifra, E.A.W. & Del C. Batlle, A.M. (1980) . Porphyrin biosynthesis in the soybean callus system-XVII. Effect of monovalent and divalent cations on the activity of succinyl CoA synthetase. International Journal of Biochemistry, 12(5-6), 717-719.
http://dx.doi.org/10.1016/0020-711X(80)90150-0
---------- CHICAGO ----------
De Xifra, E.A.W., Del C. Batlle, A.M. "Porphyrin biosynthesis in the soybean callus system-XVII. Effect of monovalent and divalent cations on the activity of succinyl CoA synthetase" . International Journal of Biochemistry 12, no. 5-6 (1980) : 717-719.
http://dx.doi.org/10.1016/0020-711X(80)90150-0
---------- MLA ----------
De Xifra, E.A.W., Del C. Batlle, A.M. "Porphyrin biosynthesis in the soybean callus system-XVII. Effect of monovalent and divalent cations on the activity of succinyl CoA synthetase" . International Journal of Biochemistry, vol. 12, no. 5-6, 1980, pp. 717-719.
http://dx.doi.org/10.1016/0020-711X(80)90150-0
---------- VANCOUVER ----------
De Xifra, E.A.W., Del C. Batlle, A.M. Porphyrin biosynthesis in the soybean callus system-XVII. Effect of monovalent and divalent cations on the activity of succinyl CoA synthetase. Int. J. Biochem. 1980;12(5-6):717-719.
http://dx.doi.org/10.1016/0020-711X(80)90150-0