Abstract:
When radiolabelled ecdysone was injected into Triatoma infestans it was converted to 20-hydroxyecdysone and to four other fractions (I, II, IV and VI) separated by thin layer chromatography. The pattern of ecdysone metabolites found 16 days after a blood meal was different from that found at 30 days. Peak I was isolated and further separated to give two main fractions. The results obtained from enzymatic hydrolysis of this peak showed it to be a mixture of glucosides, glucuronides and sulphate conjugates. © 1981.
Registro:
Documento: |
Artículo
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Título: | Ecdysone metabolism in Triatoma infestans (Hemiptera, reduviidae) in vivo |
Autor: | Stoka, A.; Charreau, E. |
Filiación: | Facultad de Ciencias Médicas de la Universidad Nacional de La Plata, Instituto de Fisiología, Cátedra de Bioquímica, calle 60 y 120, 1900 La Plata, Argentina and Centro de Estudios Parasitológicos y Vectores (CEPAVE) La Plata, Argentina Instituto de Biología y Medicina Experimental, Obligado 2490, 1428 Buenos Aires, Argentina
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Palabras clave: | conjugates; Ecdysone; Hemiptera; metabolism; Triatoma infestans |
Año: | 1981
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Volumen: | 11
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Número: | 6
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Página de inicio: | 653
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Página de fin: | 656
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DOI: |
http://dx.doi.org/10.1016/0020-1790(81)90055-X |
Título revista: | Insect Biochemistry
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ISSN: | 00201790
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00201790_v11_n6_p653_Stoka |
Referencias:
- Bray, A simple efficient liquid scintillation for counting aqueous solutions in a liquid scintillation counter (1960) Analyt. Biochem., 1, pp. 179-285
- Cerisola, Rohwedder, Segura, Del Prado, Alvarez, Winne de Martin, (1974) El xenodiagnóstico: normalización y utilidad, , Instituto Nacional Inv. Cardiovasculares e Instituto Nac. Diagn. e Inv. Enfermedad de Chagas
- Clever, Clever, Storbeck, Young, Apparent requirement of two hormones, alpha and beta ecdysone for molting in insects (1973) Devl. Biol., 31, pp. 47-60
- Cherbas, Cherbas, Distribution and metabolism of α-ecdysone in pupae of the silkworm Antheraea polyphemus (1970) Biol. Bull., 138, pp. 115-128
- Feyereisen, Lagueux, Hoffmann, Dynamics of ecdysone metabolism after injection and ingestion in Locusta migratoria (1976) Gen. comp. Endocr., 29, pp. 319-327
- Grace, Establishment of four strains of cells from insect tissues grown in vitro (1962) Nature, Lond., 195, pp. 788-789
- Gorell, Gilbert, Tash, The uptake and conversion of α-ecdysone by the pupal tissue of Hyalophora cecropia (1972) Insect Biochem., 2, pp. 94-106
- Hafferl, Wren, Marshall, Calzada, Siddall, The synthesis of tritiated α-ecdysone and 25-deoxy-α-ecdysone of high specific activity (1972) Journal of Labelled Compounds, 8, pp. 81-94
- Hoffmann, Koolman, Karlson, Joly, Molting hormone titer and metabolic fate of injected ecdysone during the fifth larval instar and in adults of Locusta migratoria (Orthoptera) (1974) Gen. comp. Endocr., 22, pp. 90-97
- Kaplanis, Thompson, Yamamoto, Robbins, Loulodes, Ecdysones from the pupa of the tobacco hornworm Manduca sexta (Johnannson) (1966) Steroids, 8, pp. 605-623
- Karlson, Koolman, On the metabolic fate of ecdysone and 3-dehydroecdysone in Calliphora vicina (1973) Insect Biochem., 3, pp. 409-417
- Koolman, Hoffmann, Karlson, Sulphate esters as in activation products of ecdysone in Locusta migratoria (1973) Hoppe-Seyler's Z. Physiol. Chem., 354, pp. 1043-1048
- Koolman, Karlson, Ecdysone oxidase and enzyme from the blowfly, Calliphora erythrocephala (Meigen) (1975) Hoppe-Seyler's Z. Physiol. Chem., 356, pp. 1131-1138
- Lagueux, Perron, Hoffmann, Ecdysone metabolism and endogenous moulting hormone titre during larval-pupal development in Choristoneura fumiferana (1976) J. Insect Physiol., 22, pp. 57-62
- Moriyama, Nakanishi, King, Okauchi, Siddal, Hafferl, On the origin and metabolic fate of α-ecdysone in insects (1970) Gen. comp. Endocr., 15, pp. 80-87
- Schaller, Hoffmann, Koolman, Metabolism de l'ecdysone chez Aeshna cyanea (Insecte, Odonate). Resultats d'injections d' α-ecdysone radioactive à des animaux et à des larves au cycle de mue bloque (1975) C.r. Acad. Sci., 280, pp. 1813-1816
- Thompson, Kaplanis, Robbins, Yamamoto, 20–26-dihydroxyecdysone, a new steroid with moulting hormone activity from the tobacco hornworm, Manduca sexta (Johnannson) (1967) Chem. Commun., pp. 650-653
- Thompson, Svoboda, Kaplanis, Robbins, Metabolic pathways of steroids in insects (1972) Proc. Roy. Soc. Ser. B, 180, pp. 203-221
- Thompson, Kaplanis, Robbins, Svoboda, Metabolism of steroids in insects (1973) Adv. Lipid Res., 11, pp. 219-265
- Thompson, Kaplanis, Robbins, Dutky, Nigg, 3-epi-20-hydroxyecdysone from meconium of the tobacco hornworm (1974) Steroids, 24, pp. 359-366
- Young, The in vivo metabolism of [3H]-molting hormones during development of Chironomus tentans and Calliphora erythrocephala (1974) Ph.D. Thesis, , Purdue University, Lafayette, Indiana
- Young, The metabolism of [3H]-molting hormone in Calliphora erythrocephala at the mature larval and white puparial stages (1976) Insect Biochem., 6, pp. 1-12
Citas:
---------- APA ----------
Stoka, A. & Charreau, E.
(1981)
. Ecdysone metabolism in Triatoma infestans (Hemiptera, reduviidae) in vivo. Insect Biochemistry, 11(6), 653-656.
http://dx.doi.org/10.1016/0020-1790(81)90055-X---------- CHICAGO ----------
Stoka, A., Charreau, E.
"Ecdysone metabolism in Triatoma infestans (Hemiptera, reduviidae) in vivo"
. Insect Biochemistry 11, no. 6
(1981) : 653-656.
http://dx.doi.org/10.1016/0020-1790(81)90055-X---------- MLA ----------
Stoka, A., Charreau, E.
"Ecdysone metabolism in Triatoma infestans (Hemiptera, reduviidae) in vivo"
. Insect Biochemistry, vol. 11, no. 6, 1981, pp. 653-656.
http://dx.doi.org/10.1016/0020-1790(81)90055-X---------- VANCOUVER ----------
Stoka, A., Charreau, E. Ecdysone metabolism in Triatoma infestans (Hemiptera, reduviidae) in vivo. 1981;11(6):653-656.
http://dx.doi.org/10.1016/0020-1790(81)90055-X