Artículo

Guerrieri, D.; Tateosian, N.L.; Maffía, P.C.; Reiteri, R.M.; Amiano, N.O.; Costa, M.J.; Villalonga, X.; Sanchez, M.L.; Estein, S.M.; Garcia, V.E.; Sallenave, J.-M.; Chuluyan, H.E. "Serine leucocyte proteinase inhibitor-treated monocyte inhibits human CD4+ lymphocyte proliferation" (2011) Immunology. 133(4):434-441
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Abstract:

Serine leucocyte proteinase inhibitor (SLPI) is the main serine proteinase inhibitor produced by epithelial cells and has been shown to be a pleiotropic molecule with anti-inflammatory and microbicidal activities. However, the role of SLPI on the adaptive immune response is not well established. Therefore, we evaluated the effect of SLPI on lymphocyte proliferation and cytokine production. Human peripheral blood mononuclear cells (PBMC) were treated with mitogens plus SLPI and proliferation was assessed by [3H]thymidine uptake. The SLPI decreased the lymphocyte proliferation induced by interleukin-2 (IL-2) or OKT3 monoclonal antibodies in a dose-dependent manner. Inhibition was not observed when depleting monocytes from the PBMC and it was restored by adding monocytes and SLPI. SLPI-treated monocyte slightly decreased MHC II and increased CD18 expression, and secreted greater amounts of IL-4, IL-6 and IL-10 in the cell culture supernatants. SLPI-treated monocyte culture supernatant inhibited the CD4+ lymphocyte proliferation but did not affect the proliferation of CD8+ cells. Moreover, IL-2 increased T-bet expression and the presence of SLPI significantly decreased it. Finally, SLPI-treated monocyte culture supernatant dramatically decreased interferon-γ but increased IL-4, IL-6 and IL-10 in the presence of IL-2-treated T cells. Our results demonstrate that SLPI target monocytes, which in turn inhibit CD4 lymphocyte proliferation and T helper type 1 cytokine secretion. Overall, these results suggest that SLPI is an alarm protein that modulates not only the innate immune response but also the adaptive immune response. © 2011 The Authors. Immunology © 2011 Blackwell Publishing Ltd.

Registro:

Documento: Artículo
Título:Serine leucocyte proteinase inhibitor-treated monocyte inhibits human CD4+ lymphocyte proliferation
Autor:Guerrieri, D.; Tateosian, N.L.; Maffía, P.C.; Reiteri, R.M.; Amiano, N.O.; Costa, M.J.; Villalonga, X.; Sanchez, M.L.; Estein, S.M.; Garcia, V.E.; Sallenave, J.-M.; Chuluyan, H.E.
Filiación:Departamento de Farmacología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
Laboratorio de Inmunología, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Tandil, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Unité de Défense Innée et Inflammation, Institut Pasteur, Paris, France
INSERM U874, Paris, France
Université Paris Diderot, Paris, France
Palabras clave:Inflammation; Innate immunity; Macrophages/monocytes; Proteases; Serine leucocyte proteinase inhibitor; T cells; T helper type 1; CD18 antigen; gamma interferon; interleukin 10; interleukin 12; interleukin 2; interleukin 4; interleukin 6; major histocompatibility antigen class 2; mitogenic agent; monoclonal antibody; OKT 3; serine leucocyte proteinase inhibtor; serine proteinase inhibitor; thymidine; unclassified drug; antigen expression; article; CD4+ T lymphocyte; CD8+ T lymphocyte; cell culture; cell proliferation; cytokine production; cytokine release; human; human cell; immune response; inflammation; peripheral blood mononuclear cell; priority journal; Th1 cell; CD4-Positive T-Lymphocytes; Cell Proliferation; Cytokines; Humans; Leukocytes, Mononuclear; Serine Proteinase Inhibitors; Structure-Activity Relationship
Año:2011
Volumen:133
Número:4
Página de inicio:434
Página de fin:441
DOI: http://dx.doi.org/10.1111/j.1365-2567.2011.03451.x
Título revista:Immunology
Título revista abreviado:Immunology
ISSN:00192805
CODEN:IMMUA
CAS:OKT 3, 140608-64-6; gamma interferon, 82115-62-6; interleukin 12, 138415-13-1; interleukin 2, 85898-30-2; thymidine, 50-89-5; Cytokines; Serine Proteinase Inhibitors
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00192805_v133_n4_p434_Guerrieri

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Citas:

---------- APA ----------
Guerrieri, D., Tateosian, N.L., Maffía, P.C., Reiteri, R.M., Amiano, N.O., Costa, M.J., Villalonga, X.,..., Chuluyan, H.E. (2011) . Serine leucocyte proteinase inhibitor-treated monocyte inhibits human CD4+ lymphocyte proliferation. Immunology, 133(4), 434-441.
http://dx.doi.org/10.1111/j.1365-2567.2011.03451.x
---------- CHICAGO ----------
Guerrieri, D., Tateosian, N.L., Maffía, P.C., Reiteri, R.M., Amiano, N.O., Costa, M.J., et al. "Serine leucocyte proteinase inhibitor-treated monocyte inhibits human CD4+ lymphocyte proliferation" . Immunology 133, no. 4 (2011) : 434-441.
http://dx.doi.org/10.1111/j.1365-2567.2011.03451.x
---------- MLA ----------
Guerrieri, D., Tateosian, N.L., Maffía, P.C., Reiteri, R.M., Amiano, N.O., Costa, M.J., et al. "Serine leucocyte proteinase inhibitor-treated monocyte inhibits human CD4+ lymphocyte proliferation" . Immunology, vol. 133, no. 4, 2011, pp. 434-441.
http://dx.doi.org/10.1111/j.1365-2567.2011.03451.x
---------- VANCOUVER ----------
Guerrieri, D., Tateosian, N.L., Maffía, P.C., Reiteri, R.M., Amiano, N.O., Costa, M.J., et al. Serine leucocyte proteinase inhibitor-treated monocyte inhibits human CD4+ lymphocyte proliferation. Immunology. 2011;133(4):434-441.
http://dx.doi.org/10.1111/j.1365-2567.2011.03451.x