Artículo
Vilariño-García, T.; Pérez-Pérez, A.; Dietrich, V.; Fernández-Sánchez, M.; Guadix, P.; Dueñas, J.L.; Varone, C.L.; Damiano, A.E.; Sánchez-Margalet, V. "Increased Expression of Aquaporin 9 in Trophoblast from Gestational Diabetic Patients" (2016) Hormone and Metabolic Research. 48(8):535-539
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Abstract:
Gestational diabetes mellitus is the most frequent pathophysiological alteration in pregnancy, increasing the incidence of complications in both mother and fetus. The macrosomia that occurs in these fetuses may be related with some changes in nutrient transport mechanism in placenta. The presence of aquaporin 9, an aquaglyceroporin, has previously been demonstrated in placenta. We raised the question whether aquaporin 9 expression may be upregulated in placenta from gestational diabetes, thus providing a faster transport of glycerol and water through placenta. We studied 21 placentas (13 controls and 8 gestational diabetes) from cesarean delivery at term. The expression of aquaporin 9 was analyzed by quantitative PCR, immunoblot, and immunohistochemistry. The median values from quantitative PCR were compared by nonparametric tests for independent samples (Mann-Whitney U-test). We have found that trophoblast from gestational diabetes express higher amount of aquaporin 9, which was found statistically significant (p<0.05). The increase in aquaporin 9 expression was confirmed by immunoblot, and localization in the syncytiotrophoblast was checked by immunohistochemistry. The increase in aquaporin 9 expression in placenta from gestational diabetes may contribute to the higher transport rate in this pathology of pregnancy. © Georg Thieme Verlag KG Stuttgart, New York.
Registro:
| Documento: | Artículo |
| Título: | Increased Expression of Aquaporin 9 in Trophoblast from Gestational Diabetic Patients |
| Autor: | Vilariño-García, T.; Pérez-Pérez, A.; Dietrich, V.; Fernández-Sánchez, M.; Guadix, P.; Dueñas, J.L.; Varone, C.L.; Damiano, A.E.; Sánchez-Margalet, V. |
| Filiación: | Department of Clinical Biochemistry, Medical School, University of Seville, Virgen Macarena University Hospital, Av. Dr. Fedriani 3, Seville, 41071, Spain Department of Biological Sciences, School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina Department of Biological Chemistry, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina Valencian Infertility Institute (IVI), Seville, Spain Obstetrics and Gynecology Department, Virgen Macarena University Hospital, Medical School, University of Seville, Seville, Spain |
| Palabras clave: | AQP9; aquaporins; gestational diabetes mellitus; placenta; aquaporin 9; glycerol; AQP9 protein, human; aquaporin; messenger RNA; adult; Article; cesarean section; clinical article; controlled study; diabetic patient; female; gene expression; human; human tissue; immunoblotting; immunohistochemistry; placenta; pregnancy diabetes mellitus; priority journal; protein expression; quantitative analysis; real time polymerase chain reaction; syncytiotrophoblast; upregulation; water transport; gene expression regulation; genetics; metabolism; pathology; pregnancy; pregnancy diabetes mellitus; protein transport; trophoblast; Adult; Aquaporins; Diabetes, Gestational; Female; Gene Expression Regulation; Humans; Pregnancy; Protein Transport; RNA, Messenger; Trophoblasts |
| Año: | 2016 |
| Volumen: | 48 |
| Número: | 8 |
| Página de inicio: | 535 |
| Página de fin: | 539 |
| DOI: | http://dx.doi.org/10.1055/s-0042-105152 |
| Título revista: | Hormone and Metabolic Research |
| Título revista abreviado: | Horm. Metab. Res. |
| ISSN: | 00185043 |
| CODEN: | HMMRA |
| CAS: | glycerol, 56-81-5; aquaporin, 215587-75-0; AQP9 protein, human; Aquaporins; RNA, Messenger |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00185043_v48_n8_p535_VilarinoGarcia |
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Citas:
---------- APA ----------
Vilariño-García, T., Pérez-Pérez, A., Dietrich, V., Fernández-Sánchez, M., Guadix, P., Dueñas, J.L., Varone, C.L.,..., Sánchez-Margalet, V. (2016) . Increased Expression of Aquaporin 9 in Trophoblast from Gestational Diabetic Patients. Hormone and Metabolic Research, 48(8), 535-539.http://dx.doi.org/10.1055/s-0042-105152
---------- CHICAGO ----------
Vilariño-García, T., Pérez-Pérez, A., Dietrich, V., Fernández-Sánchez, M., Guadix, P., Dueñas, J.L., et al. "Increased Expression of Aquaporin 9 in Trophoblast from Gestational Diabetic Patients" . Hormone and Metabolic Research 48, no. 8 (2016) : 535-539.http://dx.doi.org/10.1055/s-0042-105152
---------- MLA ----------
Vilariño-García, T., Pérez-Pérez, A., Dietrich, V., Fernández-Sánchez, M., Guadix, P., Dueñas, J.L., et al. "Increased Expression of Aquaporin 9 in Trophoblast from Gestational Diabetic Patients" . Hormone and Metabolic Research, vol. 48, no. 8, 2016, pp. 535-539.http://dx.doi.org/10.1055/s-0042-105152
---------- VANCOUVER ----------
Vilariño-García, T., Pérez-Pérez, A., Dietrich, V., Fernández-Sánchez, M., Guadix, P., Dueñas, J.L., et al. Increased Expression of Aquaporin 9 in Trophoblast from Gestational Diabetic Patients. Horm. Metab. Res. 2016;48(8):535-539.http://dx.doi.org/10.1055/s-0042-105152
