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Pérez-Pérez, A.; Guadix, P.; Maymó, J.; Dueñas, J.L.; Varone, C.; Fernández-Sánchez, M.; Sánchez-Margalet, V. "Insulin and Leptin Signaling in Placenta from Gestational Diabetic Subjects" (2015) Hormone and Metabolic Research. 48(1):62-69
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Abstract:

Insulin and leptin receptors are known to share signaling pathways, such as JAK2/STAT-3 (Janus kinase2/signal transduction and activator of transcription3), MAPK (Mitogen activated protein kinase), and PI3K (phosphoinositide 3-kinase). Both positive and negative cross-talk have been previously found in different cellular systems. Gestational diabetes (GDM) is a pathophysiological state with high circulating levels of both insulin and leptin. We have previously found that these 3 signaling pathways are activated in placenta from GDM patients to promote translation, involving the activation of leptin receptor. Now, we have tested the hypothesis that both leptin and insulin receptors might contribute to this activation in a positive way that may become negative when the system is overactivated. We studied the activation of leptin and insulin receptors in placenta from GDM and healthy pregnancies. We have also performed in vitro studies with insulin and leptin stimulation of trophoblast explants from healthy placenta. We have found that both leptin and insulin receptors are activated in placenta from GDM. In vitro stimulation of trophoblast explants with both leptin and insulin at submaximal doses (0.1 nM) potentiated the activation of signaling, whereas preincubation with maximal concentrations of insulin (10 nM) and further stimulation with leptin showed negative effect. Trophoblastic explants from GDM placenta, which presented high signaling levels, had a negative signaling effect when further incubated in vitro with leptin. In conclusion, insulin and leptin receptors have positive effects on signaling, contributing to high signaling levels in GDM placenta, but insulin and leptin have negative effects upon overstimulation. © Georg Thieme Verlag KG Stuttgart · New York.

Registro:

Documento: Artículo
Título:Insulin and Leptin Signaling in Placenta from Gestational Diabetic Subjects
Autor:Pérez-Pérez, A.; Guadix, P.; Maymó, J.; Dueñas, J.L.; Varone, C.; Fernández-Sánchez, M.; Sánchez-Margalet, V.
Filiación:Department of Medical Biochemistry and Molecular Biology, School of Medicine, Virgen Macarena University Hospital, Av. Dr. Fedriani 3, Seville, 41071, Spain
Obstetrics and Gynecology Service, Virgen Macarena University Hospital, Seville, Spain
Department of Biological Chemistry, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
IVI Seville, Seville, Spain
Palabras clave:gestational diabetes; insulin; leptin; placenta; signal transduction; insulin; insulin receptor; insulin receptor substrate; IRS1 protein, human; leptin; leptin receptor; adult; biological model; case control study; dose response; down regulation; female; human; immunohistochemistry; metabolism; phosphorylation; placenta; pregnancy; pregnancy diabetes mellitus; signal transduction; Adult; Case-Control Studies; Diabetes, Gestational; Dose-Response Relationship, Drug; Down-Regulation; Female; Humans; Immunohistochemistry; Insulin; Insulin Receptor Substrate Proteins; Leptin; Models, Biological; Phosphorylation; Placenta; Pregnancy; Receptor, Insulin; Receptors, Leptin; Signal Transduction
Año:2015
Volumen:48
Número:1
Página de inicio:62
Página de fin:69
DOI: http://dx.doi.org/10.1055/s-0035-1559722
Título revista:Hormone and Metabolic Research
Título revista abreviado:Horm. Metab. Res.
ISSN:00185043
CODEN:HMMRA
CAS:insulin, 9004-10-8; Insulin; Insulin Receptor Substrate Proteins; IRS1 protein, human; Leptin; Receptor, Insulin; Receptors, Leptin
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00185043_v48_n1_p62_PerezPerez

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Citas:

---------- APA ----------
Pérez-Pérez, A., Guadix, P., Maymó, J., Dueñas, J.L., Varone, C., Fernández-Sánchez, M. & Sánchez-Margalet, V. (2015) . Insulin and Leptin Signaling in Placenta from Gestational Diabetic Subjects. Hormone and Metabolic Research, 48(1), 62-69.
http://dx.doi.org/10.1055/s-0035-1559722
---------- CHICAGO ----------
Pérez-Pérez, A., Guadix, P., Maymó, J., Dueñas, J.L., Varone, C., Fernández-Sánchez, M., et al. "Insulin and Leptin Signaling in Placenta from Gestational Diabetic Subjects" . Hormone and Metabolic Research 48, no. 1 (2015) : 62-69.
http://dx.doi.org/10.1055/s-0035-1559722
---------- MLA ----------
Pérez-Pérez, A., Guadix, P., Maymó, J., Dueñas, J.L., Varone, C., Fernández-Sánchez, M., et al. "Insulin and Leptin Signaling in Placenta from Gestational Diabetic Subjects" . Hormone and Metabolic Research, vol. 48, no. 1, 2015, pp. 62-69.
http://dx.doi.org/10.1055/s-0035-1559722
---------- VANCOUVER ----------
Pérez-Pérez, A., Guadix, P., Maymó, J., Dueñas, J.L., Varone, C., Fernández-Sánchez, M., et al. Insulin and Leptin Signaling in Placenta from Gestational Diabetic Subjects. Horm. Metab. Res. 2015;48(1):62-69.
http://dx.doi.org/10.1055/s-0035-1559722