Artículo

Pérez-Pérez, A.; Maymó, J.L.; Gambino, Y.P.; Guadix, P.; Dueñas, J.L.; Varone, C.L.; Sánchez-Margalet, V. "Activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: Possible role of leptin" (2013) Hormone and Metabolic Research. 45(6):436-442
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Abstract:

Placentas from gestational diabetes (GDM) suffer from structural and functional changes including overgrowth. That is why we aimed to study [ 3H]-leucine incorporation into protein in addition to translation signaling in placenta from GDM. Thus, we investigated the expression of leptin and leptin receptor (LEPR), as well as the activation state of signaling proteins regulating protein synthesis, such as mTOR, S6 Kinase, EIF4E-BP1, EIF4E, and eEF2 by measuring protein phosphorylation by immunoblot. [ 3H]-Leucine incorporation into protein also was determined in trophoblastic placenta explants from GDM and control pregnancy. We found that leptin and LEPR expression are increased in placentas from GDM and the translation machinery activity as well as [3H]-leucine incorporation into protein were higher in placentas from GDM compared with placentas from control pregnancy. In conclusion, protein synthesis rate is increased in placenta from GDM patients, and this may be due, at least in part, by the activation of translation signaling. The increased expression of leptin and LEPR may contribute to these effects. These results may provide a possible mechanism for the previously observed increase in placenta growth in GDM.

Registro:

Documento: Artículo
Título:Activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: Possible role of leptin
Autor:Pérez-Pérez, A.; Maymó, J.L.; Gambino, Y.P.; Guadix, P.; Dueñas, J.L.; Varone, C.L.; Sánchez-Margalet, V.
Filiación:Departamento de Bioquímica Médica y Biología Molecular, Hospital Universitario Virgen Macarena, Universidad de Sevilla, Spain
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Servicio de Ginecología y Obstetricia, Hospital Universitario Virgen Macarena, Universidad de Sevilla, Spain
Palabras clave:EIF4EBP1; gestational diabetes; LEPR; leptin; mTOR; translation; trophoblastic cells; initiation factor 4E; leptin; leptin receptor; leucine; mammalian target of rapamycin; S6 kinase; transcription factor E2F1; adult; article; clinical article; controlled study; explant; female; human; immunoblotting; placenta; pregnancy diabetes mellitus; pregnant woman; priority journal; protein expression; protein phosphorylation; protein synthesis; signal transduction; Adult; Case-Control Studies; Diabetes, Gestational; Female; Humans; Leptin; Placenta; Pregnancy; Receptors, Leptin; Signal Transduction; Young Adult
Año:2013
Volumen:45
Número:6
Página de inicio:436
Página de fin:442
DOI: http://dx.doi.org/10.1055/s-0032-1333276
Título revista:Hormone and Metabolic Research
Título revista abreviado:Horm. Metab. Res.
ISSN:00185043
CODEN:HMMRA
CAS:leucine, 61-90-5, 7005-03-0; Leptin; Receptors, Leptin
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00185043_v45_n6_p436_PerezPerez

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Citas:

---------- APA ----------
Pérez-Pérez, A., Maymó, J.L., Gambino, Y.P., Guadix, P., Dueñas, J.L., Varone, C.L. & Sánchez-Margalet, V. (2013) . Activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: Possible role of leptin. Hormone and Metabolic Research, 45(6), 436-442.
http://dx.doi.org/10.1055/s-0032-1333276
---------- CHICAGO ----------
Pérez-Pérez, A., Maymó, J.L., Gambino, Y.P., Guadix, P., Dueñas, J.L., Varone, C.L., et al. "Activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: Possible role of leptin" . Hormone and Metabolic Research 45, no. 6 (2013) : 436-442.
http://dx.doi.org/10.1055/s-0032-1333276
---------- MLA ----------
Pérez-Pérez, A., Maymó, J.L., Gambino, Y.P., Guadix, P., Dueñas, J.L., Varone, C.L., et al. "Activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: Possible role of leptin" . Hormone and Metabolic Research, vol. 45, no. 6, 2013, pp. 436-442.
http://dx.doi.org/10.1055/s-0032-1333276
---------- VANCOUVER ----------
Pérez-Pérez, A., Maymó, J.L., Gambino, Y.P., Guadix, P., Dueñas, J.L., Varone, C.L., et al. Activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: Possible role of leptin. Horm. Metab. Res. 2013;45(6):436-442.
http://dx.doi.org/10.1055/s-0032-1333276