Artículo
Abstract:
The effect of insulin on cyclic nucleotide phosphodiesterase (PDE) in rat luteal cells was studied. Cells were obtained from PMSG/hCG primed rats and further incubated or not with insulin. The hormone produced an increase of enzyme activity after a 10 min incubation of intact cells. Maximal stimulation was achieved at 0.2 nM of insulin. Two peaks of cyclic nucleotide phosphodiesterase activity were resolved after chromatography of cell cytosolic extracts on DEAE-cellulose. These peaks (I and II) were active with cAMP as substrate but only peak I was active with cGMP. The enzyme activity of both peaks was increased in cells treated with insulin. Phosphodiesterase activity in the two peaks show two kinetic components for cAMP hydrolysis, one of high affinity (Km 2-4 μM) and the other of low affinity (47-56 μM). Treatment of the cells with insulin produced a 2 to 8 fold increase of the Vmax of these peaks. In addition after stimulation with insulin, the activation of peak I phosphodiesterase by calmodulin was less effective.
Registro:
| Documento: | Artículo |
| Título: | Regulation by insulin of cyclic nucleotide phosphodiesterase (PDE) from rat luteal cells |
| Autor: | Téllez-Iñón, M.T.; Ladenheim, R.G.; Ulloa, R.; Charreau, E.H.; Tesone, M. |
| Filiación: | Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular, 1428 Buenos Aires, Argentina |
| Palabras clave: | insulin; animal cell; dose response; drug mechanism; female genital system; nonhuman; pharmacokinetics; priority journal; rat; Animalia |
| Año: | 1987 |
| Volumen: | 19 |
| Número: | 6 |
| Página de inicio: | 249 |
| Página de fin: | 252 |
| DOI: | http://dx.doi.org/10.1055/s-2007-1011791 |
| Título revista: | Hormone and Metabolic Research |
| Título revista abreviado: | HORM. METAB. RES. |
| ISSN: | 00185043 |
| CODEN: | HMMRA |
| CAS: | insulin, 9004-10-8 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00185043_v19_n6_p249_TellezInon |
Citas:
---------- APA ----------
Téllez-Iñón, M.T., Ladenheim, R.G., Ulloa, R., Charreau, E.H. & Tesone, M. (1987) . Regulation by insulin of cyclic nucleotide phosphodiesterase (PDE) from rat luteal cells. Hormone and Metabolic Research, 19(6), 249-252.http://dx.doi.org/10.1055/s-2007-1011791
---------- CHICAGO ----------
Téllez-Iñón, M.T., Ladenheim, R.G., Ulloa, R., Charreau, E.H., Tesone, M. "Regulation by insulin of cyclic nucleotide phosphodiesterase (PDE) from rat luteal cells" . Hormone and Metabolic Research 19, no. 6 (1987) : 249-252.http://dx.doi.org/10.1055/s-2007-1011791
---------- MLA ----------
Téllez-Iñón, M.T., Ladenheim, R.G., Ulloa, R., Charreau, E.H., Tesone, M. "Regulation by insulin of cyclic nucleotide phosphodiesterase (PDE) from rat luteal cells" . Hormone and Metabolic Research, vol. 19, no. 6, 1987, pp. 249-252.http://dx.doi.org/10.1055/s-2007-1011791
---------- VANCOUVER ----------
Téllez-Iñón, M.T., Ladenheim, R.G., Ulloa, R., Charreau, E.H., Tesone, M. Regulation by insulin of cyclic nucleotide phosphodiesterase (PDE) from rat luteal cells. HORM. METAB. RES. 1987;19(6):249-252.http://dx.doi.org/10.1055/s-2007-1011791
