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Abstract:

All cytogenetically studied scorpions present male achiasmatic meiosis and lack heteromorphic sex chromosomes. In contrast, information about female meiosis in scorpions is scarce due to the difficulty of finding meiotic cells. The genus Zabius includes three described species and no chromosome studies have been performed on it until now. We analyzed the constitutive heterochromatin distribution, NORs and telomeric sequences in mitosis and meiosis of males and females of different populations of Zabius fuscus. All specimens presented 2n = 18 holokinetic chromosomes that gradually decreased in size. Male meiosis presented nine bivalents and a polymorphism for one reciprocal translocation in one population. Telomeric signals were detected at every terminal region, confirming also the presence of a (TTAGG)n motif in Buthidae. Constitutive heterochromatin was found in three chromosome pairs at a terminal region; moreover, NORs were embedded in the heterochromatic region of the largest pair. Chromosome size and landmarks allowed us to propose the chromosomes involved in the rearrangement. In four females, cells at different prophase I stages were analyzed. We describe a diffuse stage and the presence of ring-shaped bivalents. We discuss the possible origin of these bivalents in the framework of chiasmatic or achiasmatic female meiosis. These results contribute to increase the scarce evidence of female meiosis in scorpions and raise new questions about its mechanism. © 2015, Springer International Publishing Switzerland.

Registro:

Documento: Artículo
Título:Male and female meiosis in the mountain scorpion Zabius fuscus (Scorpiones, Buthidae): heterochromatin, rDNA and TTAGG telomeric repeats
Autor:Adilardi, R.S.; Ojanguren-Affilastro, A.A.; Mattoni, C.I.; Mola, L.M.
Filiación:Laboratorio de Citogenética y Evolución, Departamento de Ecología, Genética y Evolución, IEGEBA (CONICET-UBA), Universidad de Buenos Aires, Intendente Güiraldes 2160, CABA, C1428EGA, Argentina
División de Aracnología, Museo Argentino de Ciencias Naturales “Bernardino Rivadavia”, CONICET, Ángel Gallardo 470, CABA, C1405DJR, Argentina
Laboratorio de Biología Reproductiva y Evolución (CONICET-UNC), Universidad Nacional de Córdoba, Av. Vélez Sarsfield 299, Córdoba, X5000JJC, Argentina
Palabras clave:Achiasmatic meiosis; Female diffuse stage; FISH; Holokinetic chromosomes; Telomere; Buthidae; Scorpiones; heterochromatin; ribosome DNA; animal; chromosome banding pattern; female; fluorescence in situ hybridization; genetics; heterochromatin; karyotyping; male; meiosis; nucleotide repeat; scorpion; telomere; Animals; Chromosome Banding; DNA, Ribosomal; Female; Heterochromatin; In Situ Hybridization, Fluorescence; Karyotyping; Male; Meiosis; Repetitive Sequences, Nucleic Acid; Scorpions; Telomere
Año:2015
Volumen:143
Número:4
Página de inicio:393
Página de fin:401
DOI: http://dx.doi.org/10.1007/s10709-015-9838-1
Título revista:Genetica
Título revista abreviado:Genetica
ISSN:00166707
CODEN:GENEA
CAS:DNA, Ribosomal; Heterochromatin
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166707_v143_n4_p393_Adilardi

Referencias:

  • Ábalos, J.W., El género Zabius Thorell, 1894 (Buthidae, Scorpiones) (1953) An Inst Med Reg Univ Tucumán, 3, pp. 349-356
  • Acosta, L.E., Rosso de Ferradás, B., Arácnidos de la Provincia de Córdoba (1996) Biodiversidad de la Provincia de Córdoba, Fauna 1, pp. 71-99. , Tada IE, Bucher EH, (eds), Universidad Nacional de Río Cuarto, Río Cuarto
  • Acosta, L.E., Candido, D.M., Buckup, E.H., Brescovit, A.D., Description of Zabius gaucho (Scorpiones, Buthidae), a new species from southern Brazil, with an update about the generic diagnosis (2008) J Arachnol, 36 (3), pp. 491-501
  • Adilardi, R.S., Ojanguren-Affilastro, A.A., Martí, D.A., Mola, L.M., Cytogenetic analysis on geographically distant parthenogenetic populations of Tityus trivittatus Kraepelin, 1898 (Scorpiones, Buthidae): karyotype, constitutive heterochromatin and rDNA localization (2014) Comp Cytogenet, 8 (2), pp. 81-92. , PID: 25147621
  • Benavente, R., Wettstein, R., Ultrastructural characterization of the sex chromosomes during spermatogenesis of spiders having holocentric chromosomes and long diffuse stage (1980) Chromosoma, 77, pp. 69-82. , COI: 1:STN:280:DyaL3c7nvVajsw%3D%3D, PID: 7371451
  • Debus, B., Nodules” in the achiasmatic meiosis of Bithynia (Mollusca, Prosobranchia) (1978) Chromosoma, 69, pp. 81-92. , COI: 1:CAS:528:DyaE1MXltFOgsw%3D%3D, PID: 738164
  • González, G., Confalonieri, V., Comas, C., Naranjo, C.A., Poggio, L., GISH Genomic in situ hybridization reveals cryptic genetic differences between maize and its putative wild progenitor Zea mays subsp. parviglumis (2004) Genome, 47, pp. 947-953. , PID: 15499408
  • Guénin, H.A., Contribution a la connaissance cytologique des scorpions: les chromosomes de Buthus occitanus Amor. (I) (1961) Vie et Milieu, 12, pp. 89-96
  • Haineman, R.Z., Hughes, R.D., Reproduction, reproductive organs and meiosis in the bisexual non-parthenogenetic mite Caloglyphus mycophagus, with reference to oocyte degeneration in virgins (1970) J Morphol, 130, pp. 93-102
  • Howell, W.M., Black, D.A., Controlled silver staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method (1980) Experientia, 36, pp. 1014-1015. , COI: 1:STN:280:DyaL3M%2Fms12kug%3D%3D, PID: 6160049
  • Ijdo, J.W., Wells, R.A., Baldini, A., Reeders, S.T., Improved telomere detection using a telomere repeat probe (TTAGGG)<inf> n</inf> generated by PCR (1991) Nucl Acids Res, 19, p. 4780. , COI: 1:CAS:528:DyaK3MXmtVSgsL8%3D, PID: 1891373
  • Kahn, J., Cytotaxonomy of ticks (1964) Q J Microsc Sci, 105, pp. 123-137
  • Keyl, H.G., Zur Karyologie der Hydrachnellen (Acarina) (1957) Chromosoma, 8, pp. 719-729. , COI: 1:STN:280:DyaG1c%2Fms1Kntg%3D%3D, PID: 13523749
  • Kořínková, T., Král, J., Structure and meiotic behaviour of B chromosomes in Sphaerium corneum/S. nucleus complex (Bivalvia: Sphaeriidae) (2011) Genetica, 139, pp. 155-165. , PID: 21120681
  • Kovařík, F., Lowe, G., Plíšková, J., Šťáhlavský, F., A new scorpion genus, Gint gen. n., from the Horn of Africa (Scorpiones: Buthidae) (2013) Euscorpius, 173, 19p
  • Král, J., Musilová, J., Šťáhlavský, F., Rezác, M., Akan, Z., Edwards, R.L., Coyle, F.A., Almerje, C.R., Evolution of the karyotype and sex chromosome systems in basal clades of araneomorph spiders (2006) Chromosome Res, 14, pp. 859-880. , PID: 17195053
  • Král, J., Kováč, L., Šťáhlavský, F., Lonský, P., L´uptáčik, P., The first karyotype study in palpigrades, a primitive order of arachnids (Arachnida: Palpigradi) (2008) Genetica, 134, pp. 79-87. , PID: 18030430
  • Král, J., Kořínková, T., Krkavcová, L., Musilová, J., Forman, M., Ávila Herrera, I.M., Haddad, C.R., Hedin, M., Evolution of karyotype, sex chromosomes, and meiosis in mygalomorph spiders (Araneae: Mygalomorphae) (2013) Biol J Linn Soc, 109, pp. 377-408
  • López-Fernández, C., Pradillo, E., Zabal-Augirre, M., Fernandez, J.L., Garcia De La Vega, C., Gisalvez, J., Telomeric and interstitial telomeric-like DNA sequence in Orthoptera genomes (2004) Genome, 47, pp. 757-763. , PID: 15284881
  • Marec, F., Synaptonemal complexes in insects (1996) Int J Insect Morphol Embryol, 25, pp. 205-233
  • Mattos, V.F., Cella, D.M., Carvalho, L.S., Candido, D.M., Schneider, M.C., High chromosome variability and the presence of multivalent associations in buthid scorpions (2013) Chromosome Res, 21, pp. 121-136. , COI: 1:CAS:528:DC%2BC3sXlvV2mtrs%3D, PID: 23494776
  • McKee, B.D., Yan, R., Tsai, J., Meiosis in male Drosophila (2012) Spermatogenesis, 2, pp. 167-184. , PID: 23087836
  • Moustafa, M.A., Alaa, A.M., Sarlan, M.H., Yaseen, A.E., Chromosomal studies on four Egyptian scorpion species of genus Androctonus (Family: Buthidae) (2005) Cytologia, 70, pp. 161-165
  • Nath, V., Cell inclusions in the oogenesis of scorpions (1925) Proc R Soc Lond B, 98, pp. 44-58
  • Oakley, H.A., Meiosis in Mesostoma ehrenbergii ehrenbergii (Turbellaria, Rhabdocoela) (1982) Chromosoma, 87, pp. 133-147
  • Ojanguren-Affilastro, A.A., Estudio monográfico de los escorpiones de la República Argentina (2005) Rev Iber Aracnol, 11, pp. 75-241
  • Peretti, A.V., Comportamiento de apareamiento de Zabius fuscus (Thorell) (Scorpiones, Buthidae) (1991) Bol Soc Biol Concepc, 62, pp. 123-146
  • Peretti, A.V., Estudio de la biología reproductiva en escorpiones argentinos (Arachnida, Scorpiones). (morfología funcional y comportamiento). PhD thesis, Facultad de Ciencias Exactas (1993) Físicas y Naturales, , Universidad Nacional de Córdoba, Argentina
  • Peretti, A.V., Carrera, P., Female control of mating sequences in the mountain scorpion Zabius fuscus: males do not use coercion as a response to unreceptive females (2005) Anim Behav, 69, pp. 453-462
  • Piza, S.T., Comportamento dos cromossomos na primeira divisão do espermatócito do Tityus bahiensis (1939) Sci Genet, 1, pp. 255-261
  • Rodríguez Gil, S.G., Mola, L.M., Papeschi, A.G., Scioscia, C.L., Cytogenetic heterogeneity in common haplogyne spiders from Argentina (Arachnida, Araneae) (2002) J Arachnol, 30, pp. 47-56
  • Sato, I., Studies on the cytoplasmic phenomena in the spermatogenesis of the oriental scorpion, Buthus martensii, with special reference to the structure of the chondriosome ring and the dictyokinesis (1940) J Sci Hiroshima Univ, 8, 116p
  • Schneider, M.C., Cella, D.M., Karyotype conservation in 2 populations of the parthenogenetic scorpion Tityus serrulatus (Buthidae): rDNA and its associated heterochromatin are concentrated on only one chromosome (2010) J Hered, 101, pp. 491-496. , COI: 1:CAS:528:DC%2BC3cXnvVOls7k%3D, PID: 20231264
  • Schneider, M.C., Zacaro, A.A., Pinto-da-Rocha, R., Candido, D.M., Cella, D.M., A comparative cytogenetic analysis of 2 Bothriuridae species and overview of the chromosome data of Scorpiones (2009) J Hered, 5, pp. 545-555
  • Schneider, M.C., Zacaro, A.A., Pinto-da-Rocha, R., Candido, D.M., Cella, D.M., Complex meiotic configuration of the holocentric chromosomes: the intriguing case of the scorpion Tityus bahiensis (2009) Chromosome Res, 17, pp. 883-898. , COI: 1:CAS:528:DC%2BD1MXhtl2ksL3L, PID: 19760509
  • Shanahan, C.M., Cytogenetics of Australian scorpions. I. Interchange polymorphism in the family Buthidae (1989) Genome, 32, pp. 882-889
  • Shanahan, C.M., Cytogenetics of Australian scorpions. II. Chromosome polymorphism in species of Urodacus (family Scorpionidae) (1989) Genome, 32, pp. 890-900
  • Sharma, G.P., Dutta, G.P., On the male heterogamety in Melanopa unicolor Roewer (Opiliones–Arachnida) (1959) Res Bull Panjab Univ, 10, pp. 209-213
  • Sharma, G.P., Parshad, R., Joneja, M.G., Chromosome mechanism in the males of three species of scorpions (Scorpiones–Buthidae) (1959) Res Bull Panjab Univ, 10, pp. 197-207
  • Soleglad, M.E., Fet, V., High-level systematics and phylogeny of the extant scorpions (Scorpiones: Orthosterni) (2003) Euscorpius, 11, 175p
  • Šťáhlavský, F., Král, J., Karyotype analysis and achiasmatic meiosis in pseudoscorpions of the family Chthoniidae (Arachnida: Pseudoscorpiones) (2004) Hereditas, 140, pp. 49-60. , PID: 15032947
  • Šťáhlavský, F., Král, J., Harvey, M.S., Haddad, C.R., A karyotype study on the pseudoscorpion families Geogarypidae, Garypinidae and Olpiidae (Arachnida: Pseudoscorpiones) (2006) Eur J Entomol, 103, pp. 277-289
  • Sumner, A.T., A simple technique for demonstrating centromeric heterochromatin (1972) Exp Cell Res, 75, pp. 304-306. , COI: 1:STN:280:DyaE3s%2FkslGitA%3D%3D, PID: 4117921
  • Suzuki, S., Cytological studies in spiders III. Studies on the chromosomes of fifty-seven species of spiders belonging to seventeen families with general considerations on chromosomal evolution (1954) J Sci Hiroshima Univ (B), 15, pp. 23-136
  • Teruel, R., Confirmación de la presencia del género Zabius Thorell 1894 (Scorpiones: Buthidae) en la provincia de Tucumán, Argentina (2002) Rev Iber Aracnol, 6, pp. 147-148
  • Vítková, M., Král, J., Traut, W., Zrzavý, J., Marec, F., The evolutionary origin of insect telomeric repeats, (TTAGG)<inf> n</inf> (2005) Chromosome Res, 13, pp. 145-156. , PID: 15861304
  • White, M.J.D., (1973) Animal cytology and evolution, , Cambridge University Press, Cambridge
  • Wilson, E.B., The distribution of sperm-forming materials in scorpions (1931) J Morphol, 52, pp. 429-483
  • Yamazaki, K., Yahata, H., Kobayashi, N., Makioka, T., Egg maturation and parthenogenetic recovery of diploidy in the scorpion Liocheles australasiae (Fabricius) (Scorpions, Ischnuridae) (2001) J Morphol, 247, pp. 39-50. , COI: 1:STN:280:DC%2BD3M7gs1Wrsw%3D%3D, PID: 11124685
  • Yoshida, Y., Toshioka, S., Studies on spermatogenesis in scorpions. I. Numbers of chromosomes in male germ-cells of three species of scorpions (1964) Acta Arachnol, 19, 4p

Citas:

---------- APA ----------
Adilardi, R.S., Ojanguren-Affilastro, A.A., Mattoni, C.I. & Mola, L.M. (2015) . Male and female meiosis in the mountain scorpion Zabius fuscus (Scorpiones, Buthidae): heterochromatin, rDNA and TTAGG telomeric repeats. Genetica, 143(4), 393-401.
http://dx.doi.org/10.1007/s10709-015-9838-1
---------- CHICAGO ----------
Adilardi, R.S., Ojanguren-Affilastro, A.A., Mattoni, C.I., Mola, L.M. "Male and female meiosis in the mountain scorpion Zabius fuscus (Scorpiones, Buthidae): heterochromatin, rDNA and TTAGG telomeric repeats" . Genetica 143, no. 4 (2015) : 393-401.
http://dx.doi.org/10.1007/s10709-015-9838-1
---------- MLA ----------
Adilardi, R.S., Ojanguren-Affilastro, A.A., Mattoni, C.I., Mola, L.M. "Male and female meiosis in the mountain scorpion Zabius fuscus (Scorpiones, Buthidae): heterochromatin, rDNA and TTAGG telomeric repeats" . Genetica, vol. 143, no. 4, 2015, pp. 393-401.
http://dx.doi.org/10.1007/s10709-015-9838-1
---------- VANCOUVER ----------
Adilardi, R.S., Ojanguren-Affilastro, A.A., Mattoni, C.I., Mola, L.M. Male and female meiosis in the mountain scorpion Zabius fuscus (Scorpiones, Buthidae): heterochromatin, rDNA and TTAGG telomeric repeats. Genetica. 2015;143(4):393-401.
http://dx.doi.org/10.1007/s10709-015-9838-1