Artículo

El editor solo permite decargar el artículo en su versión post-print desde el repositorio. Por favor, si usted posee dicha versión, enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

The “yerba mate” tree, Ilex paraguariensis St. Hil., is a crop native to subtropical South America, marketed for the elaboration of the highly popular “mate” beverage. The Uruguayan germplasm occupies the southernmost area of the species distribution range and carries adaptations to environments that considerably differ from the current production area. We characterized the genetic variability of the germplasm from this unexplored area by jointly analyzing individuals from the diversification center (ABP, Argentina, Brazil and Paraguay) with 19 nuclear and 11 plastidic microsatellite markers. For the Uruguayan germplasm, we registered 55 alleles (18 % private), and 80 genotypes (44 % exclusive), whereas 63 alleles (28.6 % private) and 81 genotypes (42 % exclusive) were recorded for individuals from ABP. Only two plastidic haplotypes were detected. Distance-based and multilocus genotype analyses showed that individuals from ABP intermingle and that the Uruguayan germplasm is differentiated in three gene-pools. Significant positive correlations between genetic and geographic distances were detected. Our results concur in that ABP individuals harbor greater genetic variation than those from the tail of the distribution, as to the number of alleles (1.15-fold), He (1.19-fold), Rs (1.39-fold), and the between-group genetic distances (1.16-fold). Also the shape of the genetic landscape interpolation analysis suggests that the genetic variation decays southward towards the Uruguayan territory. We showed that Uruguayan germplasm hosts a combination of nuclear alleles not present in the central region, constituting a valuable breeding resource. Future conservation efforts should concentrate in collecting numerous individuals of “yerba mate” per site to gather the existent variation. © 2014, Springer International Publishing Switzerland.

Registro:

Documento: Artículo
Título:Genetic diversity of wild germplasm of “yerba mate” (Ilex paraguariensis St. Hil.) from Uruguay
Autor:Cascales, J.; Bracco, M.; Poggio, L.; Gottlieb, A.M.
Filiación:Laboratorio de Citogenética y Evolución (LaCyE), Departamento de Ecología, Genética y Evolución, Instituto IEGEBA (CONICET-UBA), Universidad de Buenos Aires, Intendente Güiraldes y Costanera Norte s/n, 4to piso, Pabellón II, Ciudad Universitaria, Ciudad Autónoma de Buenos Aires, C1428EHA, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Avenida Rivadavia 1917, Ciudad Autónoma de Buenos Aires, C1033AAJ, Argentina
Palabras clave:Genetic diversity; Ilex paraguariensis; Nuclear and plastidic SSR; Uruguayan accessions; Croton ovalifolius; Ilex paraguariensis; chloroplast DNA; microsatellite DNA; plant DNA; allele; cell nucleus; DNA sequence; gene pool; genetic variability; genetics; genotype; haplotype; Ilex paraguariensis; population genetics; Uruguay; Alleles; Cell Nucleus; DNA, Chloroplast; DNA, Plant; Gene Pool; Genetic Variation; Genetics, Population; Genotype; Haplotypes; Ilex paraguariensis; Microsatellite Repeats; Sequence Analysis, DNA; Uruguay
Año:2014
Volumen:142
Número:6
Página de inicio:563
Página de fin:573
DOI: http://dx.doi.org/10.1007/s10709-014-9804-3
Título revista:Genetica
Título revista abreviado:Genetica
ISSN:00166707
CODEN:GENEA
CAS:DNA, Chloroplast; DNA, Plant
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166707_v142_n6_p563_Cascales

Referencias:

  • Anesini, C., Turner, S., Cogoi, L., Filip, R., Study of the participation of caffeine and polyphenols on the overall antioxidant activity of mate (Ilex paraguariensis) (2012) LWT-Food Sci Technol, 45 (2), pp. 299e-304e
  • Bassam, B.J., Caetano-Anollés, G., Greshoff, P.M., Fast and sensitive silver staining of DNA in polyacrylamide gels (1991) Anal Biochem, 196, pp. 81-84
  • Bock, R., Structure, function and inheritance of plastid genomes (2007) Cell and molecular biology of plastids, pp. 29-64. , Bock R, (ed), Springer-Verlag, Berlin Heidelberg:
  • Bryant, D., Moulton, V., Neighbor-net: an agglomerative method for the construction of phylogenetic networks (2004) Mol Biol Evol, 21, pp. 255-265. , COI: 1:CAS:528:DC%2BD2cXisFWmurs%3D, PID: 14660700
  • Chakraborty, R., Jin, L., A unified approach to study hypervariable polymorphisms: statistical considerations of determining relatedness and population distances (1993) DNA fingerprinting: state of the science, pp. 153-175. , Pena SDJ, Chakraborty R, Epplen JT, Jeffreys AJ, (eds), Birkhauser Verlag, Basel, Switzerland:
  • Earl, D.A., vonHoldt, B.M., STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method (2012) Conserv Genet Resour, 4, pp. 359-361
  • El Mousadik, A., Petit, R., High level of genetic differentiation for allelic richness among populations of the argan tree (Argania spinosa (L.) Skeels) endemic to Morocco (1996) Theor Appl Genet, 92, pp. 832-839. , PID: 24166548
  • Evanno, G., Regnaut, S., Goudet, J., Detecting the number of clusters of individuals using the software structure: a simulation study (2005) Mol Ecol, 14, pp. 2611-2620. , COI: 1:CAS:528:DC%2BD2MXmvF2qtrg%3D, PID: 15969739
  • Excoffier, L., Analysis of population subdivision (2007) Handbook of statistical genetics, pp. 980-1020. , Balding D, Bishop M, Cannings C, (eds), Wiley, New York:
  • Excoffier, L., Smouse, P., Quattro, J., Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data (1992) Genetics, 131, pp. 479-491. , COI: 1:CAS:528:DyaK38XlsVCntro%3D, PID: 1644282
  • Falush, D., Stephens, M., Pritchard, J.K., Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies (2003) Genetics, 164, pp. 1567-1587. , COI: 1:CAS:528:DC%2BD3sXnvF2ntrk%3D, PID: 12930761
  • Filip, R., Ferraro, G.E., Bandoni, A.L., Bracesco, N., Nunes, E., Gugliucci, A., Dellacassa, E., Mate (Ilex paraguariensis) (2009) Recent advances in phytochemistry, pp. 113-131. , Imperato F, (ed), Research Signpost, Kerala, India:
  • Gauer, L., Cavalli-Molina, S., Genetic variation in natural populations of maté (Ilex paraguariensis A. St. Hil., Aquifoliaceae) using RAPD markers (2000) Heredity, 84, pp. 647-654. , COI: 1:CAS:528:DC%2BD3cXmsVSisLo%3D, PID: 10886380
  • Giberti, G.C., Diferentes aspectos del género Ilex (Aquifoliaceae). Corología, arquitectura floral, posición sistemática. Doctoral Thesis, Universidad de Buenos Aires (2001) Argentina, p. 154
  • Giberti, G.C., La “yerba mate” (Ilex paraguariensis, Aquifoliaceae) en tempranos escritos rioplatenses de Bonpland y su real distribución geográfica en Sudamérica austral (2011) Bonplandia, 20, pp. 203-212
  • Giberti, G.C., South American Ilex raw materials for regional infusions: advanced arboreal crop systems versus wild forest extractive production (2011) Multidisciplinary approaches on food science and nutrition for the XXI century, pp. 17-27. , Filip R, (ed), Kerala, India:
  • Goldstein, D.B., Ruiz Linares, A., Cavalli-Sforza, L.L., Feldman, M.W., An evaluation of genetic distances for use with microsatellite loci (1995) Genetics, 139, pp. 463-471. , COI: 1:STN:280:DyaK2M3itVKrtA%3D%3D, PID: 7705647
  • (2013) Google Earth version 7.1.2.2041, , http://www.google.com/earth
  • Gottlieb, A.M., Giberti, G.C., Poggio, L., Evaluación del germoplasma de Ilex paraguariensis e Ilex dumosa (Aquifoliaceae) (2011) Bol Soc Argent Bot, 46, pp. 113-123
  • Goudet, J., FSTAT (version 1.2): a computer program to calculate F-statistics (1995) J Hered, 86, pp. 485-486
  • Goudet, J., FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3) (2001) Université de Lausanne, Lausanne, , http://www2.unil.ch/popgen
  • Grela, I.A., Geografía florística de las especies arbóreas de Uruguay: propuesta para la delimitación de dendrofloras. Msc thesis, Universidad de la República, Montevideo (2004) Uruguay, p. 97
  • Hall, T.A., BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT (1999) Nucleic Acids Symp Series, 41, pp. 95-98. , COI: 1:CAS:528:DC%2BD3cXhtVyjs7Y%3D
  • Heck, C.I., Gonzalez De Mejia, E., Yerba mate tea (Ilex paraguariensis): a comprehensive review on chemistry, health implications, and technological considerations (2007) J Food Sci, 72, pp. R138-R151. , COI: 1:CAS:528:DC%2BD2sXhsValtb3N, PID: 18034743
  • Huson, D.H., Bryant, D., Application of phylogenetic networks in evolutionary studies (2006) Mol Biol Evol, 23, pp. 254-267. , COI: 1:CAS:528:DC%2BD28XntValsw%3D%3D, PID: 16221896
  • Erba-Mate, I.B., Mateando. Informativo do IBRAMATE 1, Ilópolis (2014) Brazil, p. 15
  • (2014) Instituto Nacional de la Yerba Mate (INIM), , http://www.inym.org.ar/
  • Kaundun, S.S., Matsumoto, S., Heterologous nuclear and chloroplast microsatellite amplification and variation in tea, Camellia sinensis (2002) Genome, 45, pp. 1041-1048. , COI: 1:CAS:528:DC%2BD3sXjvFyjsQ%3D%3D, PID: 12502248
  • Liu, K., Muse, S.V., PowerMarker: an integrated analysis environment for genetic marker analysis (2005) Bioinformatics, 21, pp. 2128-2129. , COI: 1:CAS:528:DC%2BD2MXjsl2nsbg%3D, PID: 15705655
  • Miller, M.P., Alleles In Space (AIS): computer software for the joint analysis of interindividual spatial and genetic information (2005) J Hered, 96, pp. 722-724. , COI: 1:CAS:528:DC%2BD2MXht1Wis77M, PID: 16251514
  • Neumann-Wendt, S., Genética de populações em Ilex paraguariensis St. Hil. Doctoral Thesis, Universidade Federal do Paraná (2005) Brazil, p. 165
  • Peakall, R., Smouse, P.E., GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research—an update (2012) Bioinformatics, 28, pp. 2537-2539. , COI: 1:CAS:528:DC%2BC38XhsVehtbjI, PID: 22820204
  • Pereira, M.F., Ciampi, A.Y., Inglis, P.W., Souza, V.A., Azevedo, V.C.R., Shotgun sequencing for microsatellite identification in Ilex paraguariensis (Aquifoliaceae) (2013) Appl Plant Sci, , PID: 25202524
  • Pritchard, J.K., Stephens, M., Donnelly, P., Inference of population structure using multilocus genotype data (2000) Genetics, 155, pp. 945-959. , COI: 1:STN:280:DC%2BD3cvislKrtA%3D%3D, PID: 10835412
  • Provan, J., Lawrence, P., Young, G., Wright, F., Bird, R., Paglia, G.P., Cattonaro, F., Powell, W., Analysis of the genus Zea (Poaceae) using polymorphic chloroplast simple sequence repeats (1999) Plant Syst Evol, 218, pp. 245-256. , COI: 1:CAS:528:DC%2BD3cXpt12nsA%3D%3D
  • Core Team, R., R: a language and environment for statistical computing (2013) R Foundation for Statistical Computing, , http://www.R-project.org/, Vienna, Austria:
  • Rendell, S., Ennos, R.A., Chloroplast DNA diversity of the dioecious European tree Ilex aquifolium L. (English holly) (2003) Mol Ecol, 12, pp. 2681-2688. , COI: 1:CAS:528:DC%2BD2cXht1Kqurc%3D, PID: 12969471
  • Rivas, M., Sistema reproductivo y estructura genética de poblaciones de Bromus auleticus Trinius ex-Nees (Poaceae): Estudio mediante isoenzimas (2001) Agrociencia, 5, pp. 32-40
  • Sosa, P.A., González Pérez, M.A., González-González, E.A., Rivero-Santana, E., Naranjo-Cigala, A., Oostermeijer, G., Pestano-Broto, J.J., Van Hengstum, T., Biología de la conservación de endemismos vegetales de los parques nacionales canarios: caracterización genética y demográfica (2009) Proyectos de investigación en parques nacionales, pp. 2006-2009
  • Speranza, P.R., Evolutionary Patterns in the Dilatata Group (Paspalum, Poaceae): a polyploid/agamic complex (2005) Doctoral Thesis; Florida: University of Florida, p. 119
  • Speranza, P.R., Seijo, J.G., Grela, A., Solís Neffa, V.G., Chloroplast DNA variation in the Turnera sidoides L. complex (Turneraceae): biogeographical implications (2007) J Biogeogr, 34, pp. 427-436
  • StatSoft, I., STATISTICA (data analysis software system) (2001) version 6, , www.statsoft.com
  • Stumm, G., Vedder, H., Schlegel, J., A simple method for isolation of PCR fragments from silver stained polyacrylamide gels by fine needle scratching (1997) Tech Tips Online, 2, pp. 116-117
  • Torimaru, T., Tani, N., Tsumura, Y., Hiraoka, K., Tomaru, N., Development and polymorphism of simple sequence repeat DNA markers for the evergreen shrub Ilex leucoclada M (2004) Mol Ecol Notes, 4, pp. 531-533. , COI: 1:CAS:528:DC%2BD2cXnvFOktb4%3D
  • Torimaru, T., Tani, N., Tsumura, Y., Nishimura, N., Tomaru, N., Effects of kin-structured seed dispersal on the genetic structure of the clonal dioecious shrub Ilex leucolada (2007) Evolution, 61, pp. 1289-1300. , COI: 1:STN:280:DC%2BD2szjtFOqsw%3D%3D, PID: 17542840
  • Turchetto, C., Fagundes, N.J.R., Segatto, A.L.A., Kuhlemeier, C., Solís Neffa, V.G., Speranza, P.R., Bonatto, S.L., Freitas, L.B., Diversification in the South American Pampas: the genetic and morphological variation of the widespread Petunia axillaris complex (Solanaceae) (2014) Mol Ecol, 23, pp. 374-389. , COI: 1:CAS:528:DC%2BC2cXlsFygtg%3D%3D
  • Vidal, R., González, A., Gutiérrez, L., Umaña, R., Speranza, P.R., Distribución de la diversidad genética y sistema reproductivo de Stipa neesiana Trin. et Rupr (2011) Agrociencia Uruguay, 15, pp. 1-12
  • Waits, L.P., Luikart, G., Taberlet, P., Estimating the probability of identity among genotypes in natural populations: cautions and guidelines (2001) Mol Ecol, 10, pp. 249-256. , COI: 1:CAS:528:DC%2BD3MXjs1ejtbs%3D, PID: 11251803
  • Weising, K., Gardner, R.C., A set of conserved PCR primers for the analysis of simple sequence repeat polymorphisms in chloroplast genomes of dicotyledonous angiosperms (1999) Genome, 42, pp. 9-19. , COI: 1:CAS:528:DyaK1MXhsVyqsb0%3D, PID: 10207998

Citas:

---------- APA ----------
Cascales, J., Bracco, M., Poggio, L. & Gottlieb, A.M. (2014) . Genetic diversity of wild germplasm of “yerba mate” (Ilex paraguariensis St. Hil.) from Uruguay. Genetica, 142(6), 563-573.
http://dx.doi.org/10.1007/s10709-014-9804-3
---------- CHICAGO ----------
Cascales, J., Bracco, M., Poggio, L., Gottlieb, A.M. "Genetic diversity of wild germplasm of “yerba mate” (Ilex paraguariensis St. Hil.) from Uruguay" . Genetica 142, no. 6 (2014) : 563-573.
http://dx.doi.org/10.1007/s10709-014-9804-3
---------- MLA ----------
Cascales, J., Bracco, M., Poggio, L., Gottlieb, A.M. "Genetic diversity of wild germplasm of “yerba mate” (Ilex paraguariensis St. Hil.) from Uruguay" . Genetica, vol. 142, no. 6, 2014, pp. 563-573.
http://dx.doi.org/10.1007/s10709-014-9804-3
---------- VANCOUVER ----------
Cascales, J., Bracco, M., Poggio, L., Gottlieb, A.M. Genetic diversity of wild germplasm of “yerba mate” (Ilex paraguariensis St. Hil.) from Uruguay. Genetica. 2014;142(6):563-573.
http://dx.doi.org/10.1007/s10709-014-9804-3