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Abstract:

Prosopis species constitute a very important resource in arid and semiarid regions. Some species of section Algarobia hybridise and introgress naturally in areas of sympatry. According to previous isoenzymatic studies these species have high variability within populations. However, the genetic differentiation among species was very low, and these markers failed to provide diagnostic loci for species recognition. Here we analysed by the RAPD technique natural populations of Prosopis alba, P. ruscifolia, P. nigra, P. flexuosa, and P. vinalillo with the purpose of obtaining markers for species and hybrid identification, by analysing the distribution of genetic diversity within and among species. Genetic variability (H = 0.12-0.26) was similar in all populations. Genetic differentiation among populations (F ST = 0.39) was highly significant. Hierarchical analysis of genetic structure performed by Wright (1978) method and analysis of molecular variance (AMOVA) indicated that the diversity among populations within species is low (4-13%) and most genetic diversity (54-61%) occurs within populations. The differentiation among species is intermediate (26-42%) between the previous components but highly significant. Five bands provided a tool for identifying any of the species studied, with the exception of P. vinalillo. The difficulty in diagnosing this species is discussed in reference to the hypothesis of its hybrid origin.

Registro:

Documento: Artículo
Título:First report on RAPDs patterns able to differentiate some Argentinean species of section Algarobia (Prosopis, Leguminosae)
Autor:Ferreyra, L.I.; Bessega, C.; Vilardi, J.C.; Saidman, B.O.
Filiación:Depto. de Ecologia, Genet. y Evol., Facultad de Ciencias Exactas, Nat. Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
CONICET, Argentina
Carrera del Investigador Cie., Consejo Nac. de Invest. Cie. y Tec., Argentina
Palabras clave:Algarobia; Genetic differentiation; Genetic structure; Prosopis; RAPD; Species identification; Argentina; article; classification; gene frequency; genetic marker; genetic variability; genetics; geography; methodology; phylogeny; population genetics; Prosopis; random amplified polymorphic DNA; Argentina; Gene Frequency; Genetic Markers; Genetics, Population; Geography; Phylogeny; Prosopis; Random Amplified Polymorphic DNA Technique; Variation (Genetics); Embryophyta; Fabaceae; Prosopis; Prosopis alba; Prosopis flexuosa; Prosopis nigra; Prosopis ruscifolia; Prosopis vinalillo
Año:2004
Volumen:121
Número:1
Página de inicio:33
Página de fin:42
DOI: http://dx.doi.org/10.1023/B:GENE.0000019925.06080.c7
Título revista:Genetica
Título revista abreviado:Genetica
ISSN:00166707
CODEN:GENEA
CAS:Genetic Markers
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166707_v121_n1_p33_Ferreyra

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Citas:

---------- APA ----------
Ferreyra, L.I., Bessega, C., Vilardi, J.C. & Saidman, B.O. (2004) . First report on RAPDs patterns able to differentiate some Argentinean species of section Algarobia (Prosopis, Leguminosae). Genetica, 121(1), 33-42.
http://dx.doi.org/10.1023/B:GENE.0000019925.06080.c7
---------- CHICAGO ----------
Ferreyra, L.I., Bessega, C., Vilardi, J.C., Saidman, B.O. "First report on RAPDs patterns able to differentiate some Argentinean species of section Algarobia (Prosopis, Leguminosae)" . Genetica 121, no. 1 (2004) : 33-42.
http://dx.doi.org/10.1023/B:GENE.0000019925.06080.c7
---------- MLA ----------
Ferreyra, L.I., Bessega, C., Vilardi, J.C., Saidman, B.O. "First report on RAPDs patterns able to differentiate some Argentinean species of section Algarobia (Prosopis, Leguminosae)" . Genetica, vol. 121, no. 1, 2004, pp. 33-42.
http://dx.doi.org/10.1023/B:GENE.0000019925.06080.c7
---------- VANCOUVER ----------
Ferreyra, L.I., Bessega, C., Vilardi, J.C., Saidman, B.O. First report on RAPDs patterns able to differentiate some Argentinean species of section Algarobia (Prosopis, Leguminosae). Genetica. 2004;121(1):33-42.
http://dx.doi.org/10.1023/B:GENE.0000019925.06080.c7