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Abstract:

Sliced interrenal tissue from Bufo arenarum H. was incubated without exogenous substrate (to determine endogenous corticosterone (B) and progesterone), as well as in the presence of [3H]pregnenolone ([3H]Pre) and [3H]corticosterone, at 28 and at 37°C. When yields of radiometabolites were analyzed as such, [3H]Pre at 28°C was found to be a good precursor for 18-hydroxycorticosterone (18-HO-B), aldosterone (aldo), and an unknown N very similar but not equal to 19-hydroxycorticosterone. However, [3H]Pre at 28°C was not a good precursor for corticosterone. When the same tritiated substrate was incubated at 37°C, the yields of 18-HO-B and N diminished significantly, aldo remained as it was at 28°C, and B increased. [3H]B was a poor precursor to aldo, 18-HO-B, and N, irrespective of incubation temperatures, but a good precursor to material with the mobility of 11-dehydro-B and an aldo-like compound X. Measurement of endogenous B and progesterone pools followed by calculations of specific activities lead to the conclusion that these differences should not be ascribed to artifactual competitions between precursor pools for enzyme systems. This species is thus characteristic in its capacity to bypass, at least at 28°C and under the in vitro conditions employed, corticosterone during aldo biosynthesis. Furthermore, the vast ambient temperature ranges to which this and many other amphibian species are exposed and the temperature dependence observed in corticoidogenesis of B. arenarum suggest that corticoidogenic studies in these species should be carried out at more than one temperature. © 1983.

Registro:

Documento: Artículo
Título:Corticoidogenesis in Bufo arenarum H. I. In vitro biosynthesis of [3H]pregnenolone and [3H]corticosterone metabolites and of endogenous 3-oxo-4-ene intermediates at 28°C and 37°C
Autor:Ceballos, N.R.; Cozza, E.N.; Lantos, C.P.
Filiación:Laboratorio de Embriología Animal, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina
Laboratorio de Esteroides, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina
Programa de Regulación Hormonal y Metabólica, CONICET. Buenos Aires, Argentina
Palabras clave:18 hydroxycorticosterone; aldosterone; corticosterone; pregnenolone; analysis of variance; animal; article; biosynthesis; metabolism; toad; 18-Hydroxycorticosterone; Aldosterone; Analysis of Variance; Animal; Bufo arenarum; Corticosterone; Pregnenolone; Support, Non-U.S. Gov't
Año:1983
Volumen:51
Número:1
Página de inicio:138
Página de fin:147
DOI: http://dx.doi.org/10.1016/0016-6480(83)90106-5
Título revista:General and Comparative Endocrinology
Título revista abreviado:Gen. Comp. Endocrinol.
ISSN:00166480
CODEN:GCENA
CAS:18 hydroxycorticosterone, 561-65-9; aldosterone, 52-39-1, 6251-69-0; corticosterone, 50-22-6; pregnenolone, 145-13-1; 18-Hydroxycorticosterone, 561-65-9; Aldosterone, 52-39-1; Corticosterone, 50-22-6; Pregnenolone, 145-13-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v51_n1_p138_Ceballos

Referencias:

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Citas:

---------- APA ----------
Ceballos, N.R., Cozza, E.N. & Lantos, C.P. (1983) . Corticoidogenesis in Bufo arenarum H. I. In vitro biosynthesis of [3H]pregnenolone and [3H]corticosterone metabolites and of endogenous 3-oxo-4-ene intermediates at 28°C and 37°C. General and Comparative Endocrinology, 51(1), 138-147.
http://dx.doi.org/10.1016/0016-6480(83)90106-5
---------- CHICAGO ----------
Ceballos, N.R., Cozza, E.N., Lantos, C.P. "Corticoidogenesis in Bufo arenarum H. I. In vitro biosynthesis of [3H]pregnenolone and [3H]corticosterone metabolites and of endogenous 3-oxo-4-ene intermediates at 28°C and 37°C" . General and Comparative Endocrinology 51, no. 1 (1983) : 138-147.
http://dx.doi.org/10.1016/0016-6480(83)90106-5
---------- MLA ----------
Ceballos, N.R., Cozza, E.N., Lantos, C.P. "Corticoidogenesis in Bufo arenarum H. I. In vitro biosynthesis of [3H]pregnenolone and [3H]corticosterone metabolites and of endogenous 3-oxo-4-ene intermediates at 28°C and 37°C" . General and Comparative Endocrinology, vol. 51, no. 1, 1983, pp. 138-147.
http://dx.doi.org/10.1016/0016-6480(83)90106-5
---------- VANCOUVER ----------
Ceballos, N.R., Cozza, E.N., Lantos, C.P. Corticoidogenesis in Bufo arenarum H. I. In vitro biosynthesis of [3H]pregnenolone and [3H]corticosterone metabolites and of endogenous 3-oxo-4-ene intermediates at 28°C and 37°C. Gen. Comp. Endocrinol. 1983;51(1):138-147.
http://dx.doi.org/10.1016/0016-6480(83)90106-5