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For many species parental behavior is essential for the survival of the offspring. While the ultimate causes of teleost parental behavior have been widely studied, comparatively little is known about its proximate causes. The aim of this study was to analyze the yet unexplored, potential dual role of brain and gonadal aromatases, the enzymes responsible for the conversion of androgens to estrogens in the brains and gonads of teleosts, respectively, on the different stages of the maternal care period of the biparental cichlid Cichlasoma dimerus, locally known as chanchita. By immunohistochemistry we analyzed the neural distribution of brain aromatase and observed it exclusively within the forebrain, including areas involved in the regulation of parental behavior. We next analyzed the gene expression of brain aromatase in the brain, and gonadal aromatase in the ovary, of female chanchitas through the parental care period. To further characterize the physiological environment associated to maternal care, we also evaluated sex steroid levels (17β-estradiol, testosterone and 11-ketotestoterone) and ovarian follicle percentage. The onset of parental behavior specifically downregulated sex steroids synthesis and the rate of ovarian maturation, as denoted by a more than 10-fold decrease in steroid levels and delayed detection of mature follicles in females with offspring, compared to females which eggs were removed. Gene expression levels of both aromatases were independent of maternal care at the evaluated time points, even though they varied during the parental care period. © 2017 Elsevier Inc.


Documento: Artículo
Título:A game of two? Gene expression analysis of brain (cyp19a1b) and gonadal (cyp19a1a) aromatase in females of a Neotropical cichlid fish through the parental care period and removal of the offspring
Autor:Ramallo, M.R.; Honji, R.M.; Birba, A.; Morandini, L.; Varela, M.L.; Genovese, G.; Moreira, R.G.; Somoza, G.M.; Pandolfi, M.
Filiación:Laboratorio de Neuroendocrinología y Comportamiento, DBBE, IBBEA-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, CABA, Argentina
Departamento de Fisiologia do Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil
Laboratorio de Ecotoxicología Acuática, DBBE, IBBEA-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, CABA, Argentina
Laboratorio de Ictiofisiología y Acuicultura, IIB-INTECH, CONICET, UNSAM, Chascomús, Buenos Aires, Argentina
Palabras clave:Brain aromatase; Cichlasoma dimerus; Cichlids; Gonadal aromatase; Maternal care; Offspring removal; Ovary; Sex steroids; 11 ketotestoterone; estradiol; testosterone; unclassified drug; aromatase; estradiol; sex hormone; testosterone; adult; animal cell; animal experiment; Article; Cichlasoma dimerus; cichlid; controlled study; cyp19a1a gene; cyp19a1b gene; down regulation; estradiol blood level; female; forebrain; gene; gene expression; immunohistochemistry; male; maternal care; nonhuman; ovary; ovary development; ovary follicle; ovary follicle development; parental behavior; priority journal; progeny; steroidogenesis; testosterone blood level; anatomy and histology; animal; animal behavior; blood; brain; enzymology; fluorescence; gene expression regulation; genetics; metabolism; reproduction; tropic climate; Animals; Aromatase; Behavior, Animal; Brain; Cichlids; Estradiol; Female; Fluorescence; Gene Expression Regulation, Enzymologic; Gonadal Steroid Hormones; Male; Ovary; Reproduction; Testosterone; Tropical Climate
Página de inicio:119
Página de fin:129
Título revista:General and Comparative Endocrinology
Título revista abreviado:Gen. Comp. Endocrinol.
CAS:estradiol, 50-28-2; testosterone, 58-22-0; aromatase, 9039-48-9; Aromatase; Estradiol; Gonadal Steroid Hormones; Testosterone


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---------- APA ----------
Ramallo, M.R., Honji, R.M., Birba, A., Morandini, L., Varela, M.L., Genovese, G., Moreira, R.G.,..., Pandolfi, M. (2017) . A game of two? Gene expression analysis of brain (cyp19a1b) and gonadal (cyp19a1a) aromatase in females of a Neotropical cichlid fish through the parental care period and removal of the offspring. General and Comparative Endocrinology, 252, 119-129.
---------- CHICAGO ----------
Ramallo, M.R., Honji, R.M., Birba, A., Morandini, L., Varela, M.L., Genovese, G., et al. "A game of two? Gene expression analysis of brain (cyp19a1b) and gonadal (cyp19a1a) aromatase in females of a Neotropical cichlid fish through the parental care period and removal of the offspring" . General and Comparative Endocrinology 252 (2017) : 119-129.
---------- MLA ----------
Ramallo, M.R., Honji, R.M., Birba, A., Morandini, L., Varela, M.L., Genovese, G., et al. "A game of two? Gene expression analysis of brain (cyp19a1b) and gonadal (cyp19a1a) aromatase in females of a Neotropical cichlid fish through the parental care period and removal of the offspring" . General and Comparative Endocrinology, vol. 252, 2017, pp. 119-129.
---------- VANCOUVER ----------
Ramallo, M.R., Honji, R.M., Birba, A., Morandini, L., Varela, M.L., Genovese, G., et al. A game of two? Gene expression analysis of brain (cyp19a1b) and gonadal (cyp19a1a) aromatase in females of a Neotropical cichlid fish through the parental care period and removal of the offspring. Gen. Comp. Endocrinol. 2017;252:119-129.