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Abstract:

Estrogens inhibit androgen production and this negative action on amphibian steroidogenesis could be related to the regulation of steroidogenic enzymes. Estrogens are also involved in the regulation of amphibian spermatogenesis by controlling testicular apoptosis and spermatogonial proliferation. The Bidder's organ (BO) is a structure characteristic from the Bufonidae family and in adult males of Rhinella arenarum it is one of the main sources of plasma estradiol (E2). The purpose of this study is to analyze the effect of E2 on testicular steroidogenic enzymes, apoptosis and proliferation in the toad R. arenarum. For this purpose, testicular fragments were treated during 24h with or without 2 or 20nM of E2. After treatments, the activities of cytochrome P450 17α-hydroxylase-C17-20 lyase (CypP450c17) and 3β-hydroxysteroid dehydrogenase/isomerase (3β-HSD/I) were measured by the transformation of radioactive substrates into products, and CypP450c17 expression was determined by Western blot analysis. Apoptosis in testicular sections was detected with a commercial fluorescent kit based on TUNEL method, and proliferation was evaluated by BrdU incorporation. Results indicate that E2 has no effect on CypP450c17 protein levels or enzymatic activity, while it reduces 3β-HSD/I activity during the post reproductive season. Furthermore, although E2 has no effect on apoptosis during the pre and the post reproductive seasons, it stimulates testicular apoptosis during the reproductive season, mostly in spermatocytes. Finally, E2 has no effect on testicular proliferation all year long. Taken together, these results suggest that E2 is involved in the regulation of testicular steroidogenesis and spermatogenesis. © 2015 Elsevier Inc.

Registro:

Documento: Artículo
Título:Effect of estradiol on apoptosis, proliferation and steroidogenic enzymes in the testes of the toad Rhinella arenarum (Amphibia, Anura)
Autor:Scaia, M.F.; Volonteri, M.C.; Czuchlej, S.C.; Ceballos, N.R.
Filiación:Laboratorio de Endocrinología Comparada, Departamento de Biodiversidad y Biologia Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Tecnicas, Buenos Aires, Argentina
Palabras clave:Apoptosis; Estradiol; Proliferation; Steroidogenesis; Testes; Toad; 3 beta hydroxysteroid dehydrogenase isomerase; 3beta hydroxy delta5 steroid dehydrogenase; broxuridine; cytochrome P450 17alpha hydroxylase carbon 17 20 lyase; estradiol; lyase; unclassified drug; 3 (or 17)-beta-hydroxysteroid dehydrogenase; 3(or 17)beta hydroxysteroid dehydrogenase; androgen; aromatase; estradiol; hydroxysteroid dehydrogenase; steroid 17alpha monooxygenase; adult; animal cell; Anura; apoptosis; Article; cell proliferation; controlled study; enzyme activity; estradiol blood level; fluorescence analysis; male; nonhuman; priority journal; protein expression; Rhinella arenarum; steroidogenesis; testis; TUNEL assay; Western blotting; animal; apoptosis; Bufo arenarum; cell proliferation; cytology; drug effects; growth, development and aging; metabolism; physiology; radioimmunoassay; reproduction; spermatogenesis; testis; 17-Hydroxysteroid Dehydrogenases; 3-Hydroxysteroid Dehydrogenases; Androgens; Animals; Apoptosis; Aromatase; Bufo arenarum; Cell Proliferation; Estradiol; Male; Radioimmunoassay; Reproduction; Spermatogenesis; Steroid 17-alpha-Hydroxylase; Testis
Año:2015
Volumen:221
Página de inicio:244
Página de fin:254
DOI: http://dx.doi.org/10.1016/j.ygcen.2014.12.016
Título revista:General and Comparative Endocrinology
Título revista abreviado:Gen. Comp. Endocrinol.
ISSN:00166480
CODEN:GCENA
CAS:3beta hydroxy delta5 steroid dehydrogenase, 9044-85-3; broxuridine, 59-14-3; estradiol, 50-28-2; lyase, 9055-04-3; 3(or 17)beta hydroxysteroid dehydrogenase, 9015-81-0; aromatase, 9039-48-9; hydroxysteroid dehydrogenase, 9001-56-3; steroid 17alpha monooxygenase, 189355-97-3, 9029-67-8, 9044-50-2, 331947-43-4, 9068-00-2; 17-Hydroxysteroid Dehydrogenases; 3 (or 17)-beta-hydroxysteroid dehydrogenase; 3-Hydroxysteroid Dehydrogenases; Androgens; Aromatase; Estradiol; Steroid 17-alpha-Hydroxylase
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v221_n_p244_Scaia

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Citas:

---------- APA ----------
Scaia, M.F., Volonteri, M.C., Czuchlej, S.C. & Ceballos, N.R. (2015) . Effect of estradiol on apoptosis, proliferation and steroidogenic enzymes in the testes of the toad Rhinella arenarum (Amphibia, Anura). General and Comparative Endocrinology, 221, 244-254.
http://dx.doi.org/10.1016/j.ygcen.2014.12.016
---------- CHICAGO ----------
Scaia, M.F., Volonteri, M.C., Czuchlej, S.C., Ceballos, N.R. "Effect of estradiol on apoptosis, proliferation and steroidogenic enzymes in the testes of the toad Rhinella arenarum (Amphibia, Anura)" . General and Comparative Endocrinology 221 (2015) : 244-254.
http://dx.doi.org/10.1016/j.ygcen.2014.12.016
---------- MLA ----------
Scaia, M.F., Volonteri, M.C., Czuchlej, S.C., Ceballos, N.R. "Effect of estradiol on apoptosis, proliferation and steroidogenic enzymes in the testes of the toad Rhinella arenarum (Amphibia, Anura)" . General and Comparative Endocrinology, vol. 221, 2015, pp. 244-254.
http://dx.doi.org/10.1016/j.ygcen.2014.12.016
---------- VANCOUVER ----------
Scaia, M.F., Volonteri, M.C., Czuchlej, S.C., Ceballos, N.R. Effect of estradiol on apoptosis, proliferation and steroidogenic enzymes in the testes of the toad Rhinella arenarum (Amphibia, Anura). Gen. Comp. Endocrinol. 2015;221:244-254.
http://dx.doi.org/10.1016/j.ygcen.2014.12.016