Pérez Sirkin, D.I.; Cánepa, M.M.; Fossati, M.; Fernandino, J.I.; Delgadin, T.; Canosa, L.F.; Somoza, G.M.; Vissio, P.G. "Melanin concentrating hormone (MCH) is involved in the regulation of growth hormone in Cichlasoma dimerus (Cichlidae, Teleostei)" (2012) General and Comparative Endocrinology. 176(1):102-111
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Growth hormone (GH) is the main pituitary hormone involved in somatic growth. In fish, the neuroendocrine control of GH is multifactorial due to the interaction of multiple inhibitors and stimulators. Melanin-concentrating hormone (MCH) is a cyclic peptide involved in skin color regulation of fish. In addition, MCH has been related to the regulation of food intake in both mammals and fish. There is only one report presenting evidences on the GH release stimulation by MCH in mammals in experiments in vitro, but there are no data on non-mammals. In the present work, we report for the first time the sequence of MCH and GH cDNA in Cichlasoma dimerus, a freshwater South American cichlid fish. We detected contacts between MCH fibers and GH cells in the proximal pars distalis region of the pituitary gland by double label confocal immunofluorescence indicating a possible functional relationship. Besides, we found that MCH increased GH transcript levels and stimulated GH release in pituitary cultures. Additionally, C. dimerus exposed to a white background had a greater number of MCH neurons with a larger nuclear area and higher levels of MCH transcript than those fish exposed to a black background. Furthermore, fish reared for 3. months in a white background showed a greater body weight and total length compared to those from black background suggesting that MCH might be related to somatic growth in C. dimerus. Our results report for the first time, that MCH is involved in the regulation of the synthesis and release of GH in vitro in C. dimerus, and probably in the fish growth rate. © 2012 Elsevier Inc.


Documento: Artículo
Título:Melanin concentrating hormone (MCH) is involved in the regulation of growth hormone in Cichlasoma dimerus (Cichlidae, Teleostei)
Autor:Pérez Sirkin, D.I.; Cánepa, M.M.; Fossati, M.; Fernandino, J.I.; Delgadin, T.; Canosa, L.F.; Somoza, G.M.; Vissio, P.G.
Filiación:Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina
Instituto de Investigaciones Biotecnológicas, Instituto Tecnológico de Chascomús (CONICET-UNSAM), Chascomús, Buenos Aires, Argentina
School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA 5001, Australia
Palabras clave:Cichlid; Growth hormone; Melanin concentrating hormone; Pituitary; Teleost; complementary DNA; growth hormone; melanin concentrating hormone; animal experiment; animal tissue; article; body growth; body height; body weight; Cichlasoma dimerus; cichlid; controlled study; female; hormonal regulation; hormone action; hormone determination; hormone release; hormone structure; hormone synthesis; hypophysis pars distalis; male; nonhuman; nucleotide sequence; priority journal
Página de inicio:102
Página de fin:111
Título revista:General and Comparative Endocrinology
Título revista abreviado:Gen. Comp. Endocrinol.
CAS:growth hormone, 36992-73-1, 37267-05-3, 66419-50-9, 9002-72-6; melanin concentrating hormone, 67382-96-1


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---------- APA ----------
Pérez Sirkin, D.I., Cánepa, M.M., Fossati, M., Fernandino, J.I., Delgadin, T., Canosa, L.F., Somoza, G.M.,..., Vissio, P.G. (2012) . Melanin concentrating hormone (MCH) is involved in the regulation of growth hormone in Cichlasoma dimerus (Cichlidae, Teleostei). General and Comparative Endocrinology, 176(1), 102-111.
---------- CHICAGO ----------
Pérez Sirkin, D.I., Cánepa, M.M., Fossati, M., Fernandino, J.I., Delgadin, T., Canosa, L.F., et al. "Melanin concentrating hormone (MCH) is involved in the regulation of growth hormone in Cichlasoma dimerus (Cichlidae, Teleostei)" . General and Comparative Endocrinology 176, no. 1 (2012) : 102-111.
---------- MLA ----------
Pérez Sirkin, D.I., Cánepa, M.M., Fossati, M., Fernandino, J.I., Delgadin, T., Canosa, L.F., et al. "Melanin concentrating hormone (MCH) is involved in the regulation of growth hormone in Cichlasoma dimerus (Cichlidae, Teleostei)" . General and Comparative Endocrinology, vol. 176, no. 1, 2012, pp. 102-111.
---------- VANCOUVER ----------
Pérez Sirkin, D.I., Cánepa, M.M., Fossati, M., Fernandino, J.I., Delgadin, T., Canosa, L.F., et al. Melanin concentrating hormone (MCH) is involved in the regulation of growth hormone in Cichlasoma dimerus (Cichlidae, Teleostei). Gen. Comp. Endocrinol. 2012;176(1):102-111.