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Abstract:

In Rhinella arenarum spermiation occurs as a consequence of LH/FSH increase during the amplexus or by a single dose of hCG, among other gonadotropins. The present study employs an in vitro system to study the mechanism of action of hCG in the spermiation of R. arenarum. Testicular fragments were incubated for 2h at 28°C in the presence or absence of 20IU hCG with or without different PKA/PKC inhibitors and activators as well as ouabain and amiloride as Na+/K+ ATPase and transcellular Na+ transport inhibitors, respectively. Ouabain did not induce spermiation in absence of hCG and inhibited hCG-induced spermiation in a dose-dependent manner, reaching 90% inhibition with the higher concentration. In contrast, amiloride neither affected spermiation nor steroidogenesis. Activation of PKA with 8Br-cAMP induced spermiation in the absence of hCG while its inhibition with H89 blocked hCG action. On the other hand, PKC inhibition with Bi or STP did not affect hCG-induced spermiation although PKC activation significantly decreased hCG-dependent sperm release. These results suggest that PKC inhibits spermiation but also that the inhibition exerted by the kinase could be blocked by hCG. Taken together, these observations could indicate that PKA is involved in the mechanism of the gonadotropin action, mechanism also requiring the activation of a non-pumping Na+/K+ ATPase pathway. © 2010 Elsevier Inc.

Registro:

Documento: Artículo
Título:Mechanism of hCG-induced spermiation in the toad Rhinella arenarum (Amphibia, Anura)
Autor:Volonteri, M.C.; Ceballos, N.R.
Filiación:Laboratorio de Endocrinología Comparada, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:HCG; PKA; Spermiation; Toad; adenosine triphosphatase (potassium sodium); amiloride; chorionic gonadotropin; cyclic AMP dependent protein kinase; cyclic AMP dependent protein kinase inhibitor; ouabain; protein kinase C inhibitor; Amphibia; animal tissue; article; concentration response; controlled study; enzyme activation; enzyme activity; enzyme inhibition; hormone action; hormone release; in vitro study; male; nonhuman; priority journal; Rhinella arenarum; sodium transport; spermatogenesis; spermiation; steroidogenesis
Año:2010
Volumen:169
Número:3
Página de inicio:197
Página de fin:202
DOI: http://dx.doi.org/10.1016/j.ygcen.2010.08.011
Título revista:General and Comparative Endocrinology
Título revista abreviado:Gen. Comp. Endocrinol.
ISSN:00166480
CODEN:GCENA
CAS:adenosine triphosphatase (potassium sodium); amiloride, 2016-88-8, 2609-46-3; chorionic gonadotropin, 9002-61-3; cyclic AMP dependent protein kinase; ouabain, 11018-89-6, 630-60-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v169_n3_p197_Volonteri

Referencias:

  • Beguin, P., Beggah, A.T., Chibalin, A.V., Burgener-Kairuz, P., Jaisser, F., Mathews, P.M., Rossier, B.C., Geering, K., Phosphorylation of the Na,K-ATPase α-subunit by protein kinase A and C in vitro and in intact cells (1994) J. Biol. Chem., 269, pp. 24437-24445
  • Burgos, M.H., Vitale-Calpe, R.E., The mechanism of spermiation in the toad (1967) Am. J. Anat., 120, pp. 227-252
  • Burgos, M.H., Vitale-Calpe, R.E., The fine structure of the Sertoli cell-spermatozoan relationship in the toad (1967) J. Uultrastruct. Res., 19, pp. 221-237
  • Chapin, R.E., Wine, R.N., Harris, M.W., Borchers, C.H., Haseman, J.K., Structure and control of a cell-cell adhesion complex associated with spermiation in rat seminiferous epithelium (2001) J. Androl., 22, pp. 1030-1052
  • Chen, J.J., Wang, P.S., Chien, E.J., Wang, S.W., Direct inhibitory effect of digitalis on progesterone release from rat granulosa cells (2001) Br. J. Pharmacol., 132, pp. 1761-1768
  • Cobellis, G., Cacciola, G., Chioccarelli, T., Izzo, G., Meccariello, R., Pierantoni, R., Fasano, S., Estrogen regulation of the male reproductive tract in the frog, Rana esculenta: a role in Fra-1 activation in peritubular myoid cells and in sperm release (2008) Gen. Comp. Endocrinol., 155, pp. 838-846
  • Feschenko, M.S., Sweadner, K.J., Conformation-dependent phosphorylation of Na,K-ATPase by protein kinase A and protein kinase C (1994) J. Biol. Chem., 269, pp. 30436-30444
  • Galli-Mainini, C., Reacción diagnóstica de embarazo en la que se usa el sapo macho como animal reactivo (1947) Semana Méd., 54, pp. 337-340
  • Han, F., Bossuyt, J., Despa, S., Tucker, A.L., Bers, D.M., Effects on Na +/K+ pump function in cardiac myocytes (2006) Circ. Res., 99, pp. 1376-1383
  • Ishii, S., Itoh, M., Amplexus induces surge of luteinizing hormone in male toads, Bufo japonicus (1992) Gen. Comp. Endocrinol., 86, pp. 34-41
  • Ito, R., Abé, S.-I., FSH-initiated differentiation of newt spermatogonia to primary spermatocytes in germ-somatic cell reaggregates cultured within a collagen matrix (1999) Int. J. Dev. Biol., 43, pp. 111-116
  • Ji, Z.-S., Kubokawa, K., Abé, S.-I., Promotion of differentiation of newt primary spermatocytes into spermatids by mammalian FSH via Sertoli cells (1995) J. Exp. Zool., 272, pp. 374-383
  • Kaplan, J.H., Biochemistry of Na,K-ATPase (2002) Annu. Rev. Biochem., 71, pp. 511-535
  • Kobayashi, T., Sakai, N., Adachi, S., Asahina, K., Iwasawa, H., Nagahama, Y., 17α, 20α-dihydroxy-4-pregnen-3-one is the naturally occurring spermiation-inducing hormone in the testis of a frog, Rana nigromaculata (1993) Endocrinology, 133, pp. 321-327
  • Liang, M., Tian, J., Liu, L., Pierre, S., Liu, J., Shapiro, J., Xie, Z.J., Identification of a pool of non-pumping Na+/K+ ATPase (2007) J. Biol. Chem., 282, pp. 10585-10593
  • Licht, P., Mc Creery, B.R., Barnes, R., Pang, R., Seasonal and stress-related changes in plasma gonadotropins, sex steroids, and corticosterone in the bullfrog, Rana castesbeiana (1983) Gen. Comp. Endocrinol., 50, pp. 124-145
  • Lin, H., Wang, S.W., Tsai, S.C., Chen, J.J., Chiao, Y.C., Lu, C.C., Huang, W.J., Wang, P.S., Inhibitory effect of digoxin on testosterone secretion through mechanisms involving decreases of cyclic AMP production and cytochrome P450scc activity in rat testicular interstitial cells (1998) Brit. J. Pharmacol., 125, pp. 1635-1640
  • Nagahama, Y., Testis (1986) Vertebrates Endrocrinology: Fundamentals and Biomedical Implications, 1, pp. 399-437. , Academic Press, New York
  • Newton, L.D., Krishnakumar, S., Menon, A.G., Kastelic, J.P., Thundathil, J.C., Na+/K+ ATPase regulates sperm capacitation through a mechanism involving kinases and redistribution of its testis-specific isoform (2010) Mol. Reprod. Dev., 77, pp. 136-148
  • Parvinen, M., Vihko, K.M., Toppari, J., Cell interactions during the seminiferous epithelial cycle (1986) Int. Rev. Cytol., 104, pp. 115-151
  • Pozzi, A.G., Ceballos, N.R., Human chorionic gonadotropin-induced spermiation in Bufo arenarum is not mediated by steroid biosynthesis (2000) Gen. Comp. Endocrinol., 119, pp. 164-171
  • Pozzi, A.G., Fernandez-Solari, J.J., Ceballos, N.R., Two populations cell with different responsiveness to mammalian LH, FSH and hCG from testis of Bufo arenarum (2001) Advances in Comparative Endocrinology: Unity and Diversity, pp. 1025-1030. , Monduzzi Editore, Bologna, Italy, H. Goos, R.K. Rastogi, H. Vaudry, R. Pierantoni (Eds.)
  • Pozzi, A.G., Rosemblit, C., Ceballos, N.R., Effect of human gonadotropins on spermiation and androgen biosynthesis in testes of Bufo arenarum (2006) J. Exp. Zool., 305 A, pp. 96-102
  • Pudney, J., Leydig and Sertoli cells, nonmammalian (1998) Encyclopedia of Reproduction, 2, pp. 1008-1020. , Academic Press, New York
  • Rosemblit, C., Pozzi, A.G., Ceballos, N.R., Relationship between steroidogenesis and spermiation in Rana catesbeiana and Leptodactyllus ocellatus seems to be a spread mechanism in anuran amphibians (2006) J. Comp. Physiol. B, 176, pp. 559-566
  • Russo, J., Burgos, M.H., Effect of hCG on the enzymatic activity of toad testes (1969) Gen. Comp. Endocrinol., 13, pp. 185-188
  • Steel, R.G.D., Torrie, J.H., (1980) Principles and Procedures of Statistics. A Biomedical Approach, , McGraw-Hill, New York, Chapter 9
  • Thundathil, J.C., Anzar, M., Buhr, M.M., Na+/K+ ATPase as a signalling molecule during bovine sperm capacitation (2006) Biol. Reprod., 75, pp. 308-317
  • Tian, J., Cai, T., Yuan, Z., Wang, H., Liu, L., Haas, M., Maksimova, E., Xie, Z.J., Binding of Src to Na+/K+-ATPase forms a functional signalling complex (2006) Mol. Biol. Cell, 17, pp. 317-326
  • Yamamoto, T., Nakayama, Y., Abé, S.I., Mammalian follicle-stimulating hormone and insulin-like growth factor I (IGF-I) up-regulate IGF-I gene expression in organ culture of newt testis (2001) Mol. Reprod. Dev., 60, pp. 56-60
  • Yazawa, T., Yamamoto, T., Kubokawa, K., Nakyama, Y., Fujimoto, K., Ito, R., Abé, S.-I., Cold suppression of follicle-stimulating hormone activity on proliferation and survival of newt spermatogonia (2001) Gen. Comp. Endocrinol., 122, pp. 296-303

Citas:

---------- APA ----------
Volonteri, M.C. & Ceballos, N.R. (2010) . Mechanism of hCG-induced spermiation in the toad Rhinella arenarum (Amphibia, Anura). General and Comparative Endocrinology, 169(3), 197-202.
http://dx.doi.org/10.1016/j.ygcen.2010.08.011
---------- CHICAGO ----------
Volonteri, M.C., Ceballos, N.R. "Mechanism of hCG-induced spermiation in the toad Rhinella arenarum (Amphibia, Anura)" . General and Comparative Endocrinology 169, no. 3 (2010) : 197-202.
http://dx.doi.org/10.1016/j.ygcen.2010.08.011
---------- MLA ----------
Volonteri, M.C., Ceballos, N.R. "Mechanism of hCG-induced spermiation in the toad Rhinella arenarum (Amphibia, Anura)" . General and Comparative Endocrinology, vol. 169, no. 3, 2010, pp. 197-202.
http://dx.doi.org/10.1016/j.ygcen.2010.08.011
---------- VANCOUVER ----------
Volonteri, M.C., Ceballos, N.R. Mechanism of hCG-induced spermiation in the toad Rhinella arenarum (Amphibia, Anura). Gen. Comp. Endocrinol. 2010;169(3):197-202.
http://dx.doi.org/10.1016/j.ygcen.2010.08.011