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Abstract:

This study employed an in vitro system to identify potential steroidal mediators of spermiation in Bufo arenarum. Testicular fragments were incubated for 2 h at 28°. Spermiation was induced by 10 IU human chorionic gonadotropin (hCG) and the effect of different inhibitors of steroid biosynthesis was analyzed. Cyanoketone (10-5-10-6 M), an inhibitor of 3-oxo-4-ene steroid biosynthesis, did not block hCG-inducing activity even when biosynthesis of 3-oxo-4-ene steroids and its reduced metabolites was inhibited by 95%. Aminogluthetimide at a concentration that inhibited testosterone biosynthesis (10-4 and 10-5 M) did not alter hCG actions. Similar results were obtained with spironolactone, an inhibitor of 17α-hydroxylase/17-20 lyase activity. No spermiation-inducing activity was found with different steroids (progesterone, 17-hydroxypregnenolone, 17,20α/β-dihydroxy-4-pregnene-3-one, estradiol, testosterone, etc.). It is concluded that spermiation induced by hCG is not steroid mediated in B. arenarum. (C) 2000 Academic Press.

Registro:

Documento: Artículo
Título:Human chorionic gonadotropin-induced spermiation in Bufo arenarum is not mediated by steroid biosynthesis
Autor:Pozzi, A.G.; Ceballos, N.R.
Filiación:PRHOM-CONICET and Laboratorio de Endocrinología Comparada, Departamento de Ciencias Biológicas, Universidad de Buenos Aires, C1428EHA, Buenos Aires, Argentina
Palabras clave:HCG; Spermiation; Steroids; Toad; 17 hydroxypregnenolone; 17,20beta dihydroxypregn 4 en 3 one; aminoglutethimide; chorionic gonadotropin; cyanoketone; estradiol; progesterone; spironolactone; steroid; steroid 17,20 lyase; steroid 17alpha monooxygenase; steroid hormone receptor blocking agent; testosterone; animal experiment; article; enzyme activity; enzyme inhibition; inhibition kinetics; nonhuman; priority journal; spermatogenesis; steroidogenesis; testis; toad
Año:2000
Volumen:119
Número:2
Página de inicio:164
Página de fin:171
DOI: http://dx.doi.org/10.1006/gcen.2000.7509
Título revista:General and Comparative Endocrinology
Título revista abreviado:Gen. Comp. Endocrinol.
ISSN:00166480
CODEN:GCENA
CAS:17 hydroxypregnenolone, 387-79-1; 17,20beta dihydroxypregn 4 en 3 one, 1662-06-2; aminoglutethimide, 125-84-8; chorionic gonadotropin, 9002-61-3; cyanoketone, 4248-66-2; estradiol, 50-28-2; progesterone, 57-83-0; spironolactone, 52-01-7; steroid 17alpha monooxygenase, 189355-97-3, 9029-67-8, 9044-50-2, 331947-43-4, 9068-00-2; testosterone, 58-22-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v119_n2_p164_Pozzi

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Citas:

---------- APA ----------
Pozzi, A.G. & Ceballos, N.R. (2000) . Human chorionic gonadotropin-induced spermiation in Bufo arenarum is not mediated by steroid biosynthesis. General and Comparative Endocrinology, 119(2), 164-171.
http://dx.doi.org/10.1006/gcen.2000.7509
---------- CHICAGO ----------
Pozzi, A.G., Ceballos, N.R. "Human chorionic gonadotropin-induced spermiation in Bufo arenarum is not mediated by steroid biosynthesis" . General and Comparative Endocrinology 119, no. 2 (2000) : 164-171.
http://dx.doi.org/10.1006/gcen.2000.7509
---------- MLA ----------
Pozzi, A.G., Ceballos, N.R. "Human chorionic gonadotropin-induced spermiation in Bufo arenarum is not mediated by steroid biosynthesis" . General and Comparative Endocrinology, vol. 119, no. 2, 2000, pp. 164-171.
http://dx.doi.org/10.1006/gcen.2000.7509
---------- VANCOUVER ----------
Pozzi, A.G., Ceballos, N.R. Human chorionic gonadotropin-induced spermiation in Bufo arenarum is not mediated by steroid biosynthesis. Gen. Comp. Endocrinol. 2000;119(2):164-171.
http://dx.doi.org/10.1006/gcen.2000.7509