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Abstract:

In reptiles as in other vertebrates, multiple forms of gonadotropin-releasing hormone (GnRH) within a single brain have been identified. In this group the following GnRH molecular variants have been characterized either by direct or indirect methods: chicken GnRH I (cGnRH-I), chicken GnRH II (cGnRH-II), salmon GnRH (sGnRH) and several unidentified GnRH-like forms. In the present study GnRH variants were investigated in brain extracts of the lizard Tupinambis teguixin (= T. merinae) by combining high-performance liquid chromatography (RP-HPLC) followed by radioimmunoassays (RIA). Two peaks showing GnRH immunoreactivity with the elution position of synthetic mammalian GnRH (mGnRH) and cGnRH-II were detected. Both peaks were further analyzed with different radioimmunoassay systems specific for mGnRH, cGnRH-I, and cGnRH-II. Pooled fractions corresponding to the first eluting peak showed no cross-reactivity when analyzed with a cGnRH-I specific assay and logit-log displacement curves were not significantly different from those of synthetic mGnRH with homologous RIA systems. The second peak showed immunological characteristics of cGnRH-II when analyzed with a specific antiserum. The first ir-GnRH peak was selected for further RP-HPLC purification showing similar chromatographic behavior as mGnRH synthetic standard. We demonstrated the absence of cGnRH-I in this lizard using well-characterized antisera. (C) 2000 Academic Press.

Registro:

Documento: Artículo
Título:Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed?
Autor:Montaner, A.D.; Gonzalez, O.; Paz, D.A.; Affanni, J.M.; Somoza, G.M.
Filiación:INEUCI-CONICET, Departamento de Ciencias Biológicas, Universidad de Buenos Aires, 1428, Buenos Aires, Argentina
Departamento de Zootecnia, Facultad de Agronomía, Universidad de Buenos Aires, 1417, Buenos Aires, Argentina
Laboratorio de Embriología Animal, Departamento de Ciencias Biológicas, Universidad de Buenos Aires, 1428, Buenos Aires, Argentina
Instituto de Investigaciones Biomédicas Fundación Pablo Cassará, 1440, Buenos Aires, Argentina
Palabras clave:Brain; Gonadotropin-releasing hormone; Reptiles; RIA; RP-HPLC; antiserum; brain extract; gonadorelin; animal experiment; animal tissue; article; brain; female; high performance liquid chromatography; hormone determination; hormone release; immunoreactivity; lizard; male; nonhuman; priority journal; protein expression; radioimmunoassay; reptile
Año:2000
Volumen:119
Número:2
Página de inicio:121
Página de fin:131
DOI: http://dx.doi.org/10.1006/gcen.2000.7488
Título revista:General and Comparative Endocrinology
Título revista abreviado:Gen. Comp. Endocrinol.
ISSN:00166480
CODEN:GCENA
CAS:gonadorelin, 33515-09-2, 9034-40-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v119_n2_p121_Montaner

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Citas:

---------- APA ----------
Montaner, A.D., Gonzalez, O., Paz, D.A., Affanni, J.M. & Somoza, G.M. (2000) . Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed?. General and Comparative Endocrinology, 119(2), 121-131.
http://dx.doi.org/10.1006/gcen.2000.7488
---------- CHICAGO ----------
Montaner, A.D., Gonzalez, O., Paz, D.A., Affanni, J.M., Somoza, G.M. "Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed?" . General and Comparative Endocrinology 119, no. 2 (2000) : 121-131.
http://dx.doi.org/10.1006/gcen.2000.7488
---------- MLA ----------
Montaner, A.D., Gonzalez, O., Paz, D.A., Affanni, J.M., Somoza, G.M. "Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed?" . General and Comparative Endocrinology, vol. 119, no. 2, 2000, pp. 121-131.
http://dx.doi.org/10.1006/gcen.2000.7488
---------- VANCOUVER ----------
Montaner, A.D., Gonzalez, O., Paz, D.A., Affanni, J.M., Somoza, G.M. Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed?. Gen. Comp. Endocrinol. 2000;119(2):121-131.
http://dx.doi.org/10.1006/gcen.2000.7488