Abstract:
3β-hydroxysteroid dehydrogenase 5-ene isomerase (3βHSD/I) catalyzes an essential step in the biosynthesis of steroid hormones and is usually considered to be mainly microsomal, although there is a dual distribution of the enzyme in toad interrenals. The present study demonstrates that in the testicular tissue, as in interrenals of Bufo arenarum H., 3βHSD/I is both mitochondrial and microsomal. The conversion of dehydroepiandrosterone to androstenedione takes place only in microsomes while pregnenolone is converted to progesterone in both microsomes and mitochondria. Kinetic constants of 3βHSD/I were determined by the oxidation of pregnenolone and dehydroepiandrosterone. The preferred substrate of the microsomal 3βHSD/I enzyme was dehydroepiandrosterone (K(m) = 0.17 μM and 0.53 μM for dehydroepiandrosterone and pregnenolone, respectively) not only during the breeding season but also in the nonbreeding period (K(m) = 0.49 μM and 2.9 μM for dehydroepiandrosterone and pregnenolone, respectively).
Registro:
Documento: |
Artículo
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Título: | Subcellular localization of 3β hydroxysteroid dehydrogenase isomerase in testis of Bufo arenarum H. |
Autor: | Pozzi, A.G.; Lantos, C.P.; Ceballos, N.R. |
Filiación: | PRHOM-CONICET, Universidad de Buenos Aires, 1428, Buenos Aires, Argentina Depto. de Ciencias Biológicas, Universidad de Buenos Aires, 1428, Buenos Aires, Argentina Depto. de Quim. Biológica, Universidad de Buenos Aires, 1428, Buenos Aires, Argentina Lab. de Endocrinologiia Comparada, Depto. de Ciencias Biológicas, Ciudad Universitaria, Pabellón II, 1428 Buenos Aires, Argentina
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Palabras clave: | 3(or 17)beta hydroxysteroid dehydrogenase; androstenedione; isomerase; prasterone; pregnenolone; animal tissue; article; enzyme localization; enzyme substrate; hormone synthesis; microsome; mitochondrion; nonhuman; priority journal; steroidogenesis; testis; toad |
Año: | 1997
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Volumen: | 106
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Número: | 3
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Página de inicio: | 400
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Página de fin: | 406
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DOI: |
http://dx.doi.org/10.1006/gcen.1997.6888 |
Título revista: | General and Comparative Endocrinology
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Título revista abreviado: | GEN. COMP. ENDOCRINOL.
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ISSN: | 00166480
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CODEN: | GCENA
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CAS: | 3(or 17)beta hydroxysteroid dehydrogenase, 9015-81-0; androstenedione, 26264-53-9, 63-05-8; isomerase, 9013-19-8; prasterone, 53-43-0; pregnenolone, 145-13-1
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v106_n3_p400_Pozzi |
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Citas:
---------- APA ----------
Pozzi, A.G., Lantos, C.P. & Ceballos, N.R.
(1997)
. Subcellular localization of 3β hydroxysteroid dehydrogenase isomerase in testis of Bufo arenarum H. General and Comparative Endocrinology, 106(3), 400-406.
http://dx.doi.org/10.1006/gcen.1997.6888---------- CHICAGO ----------
Pozzi, A.G., Lantos, C.P., Ceballos, N.R.
"Subcellular localization of 3β hydroxysteroid dehydrogenase isomerase in testis of Bufo arenarum H."
General and Comparative Endocrinology 106, no. 3
(1997) : 400-406.
http://dx.doi.org/10.1006/gcen.1997.6888---------- MLA ----------
Pozzi, A.G., Lantos, C.P., Ceballos, N.R.
"Subcellular localization of 3β hydroxysteroid dehydrogenase isomerase in testis of Bufo arenarum H."
General and Comparative Endocrinology, vol. 106, no. 3, 1997, pp. 400-406.
http://dx.doi.org/10.1006/gcen.1997.6888---------- VANCOUVER ----------
Pozzi, A.G., Lantos, C.P., Ceballos, N.R. Subcellular localization of 3β hydroxysteroid dehydrogenase isomerase in testis of Bufo arenarum H. GEN. COMP. ENDOCRINOL. 1997;106(3):400-406.
http://dx.doi.org/10.1006/gcen.1997.6888