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Abstract:

The enzymatic activity of 3β-hydroxysteroid dehydrogenase 5-ene isomerase (3βHSD/I) catalyzes an essential step in the biosynthesis of steroid hormones including progesterone, mineralocorticoids, glucocorticoids, estrogens, and androgens. Its subcellular localization in steroidogenic tissues is usually considered to be mainly microsomal. The present study demonstrates that in the interrenal of Bufo arenarum H., 3βHSD/I activity localizes in mitochondria and micromes. It also shows that the two distinct pathways to aldosterone previously demonstrated for interrenals of B. arenarum H. exhibit differential subcellular localizations, microsomal for the 4-ene route and mitochondrial for the 5- ene route. Kinetic constants of 3βHSD/I were determined for the oxidation of pregnenolone and the recently described 3β-hydroxy analogue of aldosterone (3βAA). The preferred substrate of the mitochondrial 3βHSD/I enzyme was 3βAA (K(m) = 0.7 μM and 14.0 μM for 3βAA and pregnenolone, respectively). However, the microsomal enzyme has a greater affinity for pregnenolone (K(m) = 0.8 μM) than for 3βAA (K(m) = 17.0). Enzymes from both localizations have similar nucleotide (NAD+) requirements, activities being higher in summer. This dual localization opens novel possibilities for the regulation of interrenal functions.

Registro:

Documento: Artículo
Título:Mitochondrial localization of 3β-hydroxysteroid dehydrogenase 5-ene isomerase in interrenals of the toad Bufo arenarum H.
Autor:Pozzi, A.G.; Lantos, C.P.; Ceballos, N.R.
Filiación:PRHOM-CONICET, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Depto. de Ciencias Biológicas, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Depto. de Quimica Biológica, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Lab. de Endocrinologia Comparada, Depto. de Ciencias Biológicas, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Palabras clave:3(or 17)beta hydroxysteroid dehydrogenase; animal tissue; article; cellular distribution; enzyme activity; enzyme localization; male; nonhuman; priority journal; steroidogenesis; tissue distribution; toad
Año:1996
Volumen:103
Número:2
Página de inicio:176
Página de fin:181
DOI: http://dx.doi.org/10.1006/gcen.1996.0108
Título revista:General and Comparative Endocrinology
Título revista abreviado:GEN. COMP. ENDOCRINOL.
ISSN:00166480
CODEN:GCENA
CAS:3(or 17)beta hydroxysteroid dehydrogenase, 9015-81-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v103_n2_p176_Pozzi

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Citas:

---------- APA ----------
Pozzi, A.G., Lantos, C.P. & Ceballos, N.R. (1996) . Mitochondrial localization of 3β-hydroxysteroid dehydrogenase 5-ene isomerase in interrenals of the toad Bufo arenarum H. General and Comparative Endocrinology, 103(2), 176-181.
http://dx.doi.org/10.1006/gcen.1996.0108
---------- CHICAGO ----------
Pozzi, A.G., Lantos, C.P., Ceballos, N.R. "Mitochondrial localization of 3β-hydroxysteroid dehydrogenase 5-ene isomerase in interrenals of the toad Bufo arenarum H." General and Comparative Endocrinology 103, no. 2 (1996) : 176-181.
http://dx.doi.org/10.1006/gcen.1996.0108
---------- MLA ----------
Pozzi, A.G., Lantos, C.P., Ceballos, N.R. "Mitochondrial localization of 3β-hydroxysteroid dehydrogenase 5-ene isomerase in interrenals of the toad Bufo arenarum H." General and Comparative Endocrinology, vol. 103, no. 2, 1996, pp. 176-181.
http://dx.doi.org/10.1006/gcen.1996.0108
---------- VANCOUVER ----------
Pozzi, A.G., Lantos, C.P., Ceballos, N.R. Mitochondrial localization of 3β-hydroxysteroid dehydrogenase 5-ene isomerase in interrenals of the toad Bufo arenarum H. GEN. COMP. ENDOCRINOL. 1996;103(2):176-181.
http://dx.doi.org/10.1006/gcen.1996.0108