Abstract:
The influence of different cultivation conditions on β-glucosidase production and of some parameters on the activity and stability of this enzyme were studied in Nectria catalinensis. Maximal β-glucosidase production was achieved with ammonium nitrate (0.5 g N/L) as nitrogen source. Tween 80, Tween 20 and Triton X-100 increased β-glucosidase yields, Tween 80 was the most effective for enzyme release and growth at a concentration of 3.4 mmol/L. On the other hand, Tween 20 and Triton X-100 had an inhibitory effect on N. catalinensis growth. A temperature of 23 °C and an initial pH of cultures of 6.5 were optimal for biomass and β-glucosidase production. Under optimal cultural conditions (ammonium nitrate, 0.5 g N/L; Tween 80, 3.4 mmol/L; 23 °C; initial pH 6.5) the β-glucosidase yield was increased more than five fold respect to the initial state. Optimal temperature for β-glucosidase activity was 45 °C, the initial activity dropped 60 % after 6 h of incubation at this temperature. Optimal PH for enzyme activity was 5.3. At this pH the β-glucosidase was completely stable after 3 d of incubation The V and Km values calculated from Lineweaver-Burk and Eadie-Hofstee plots were 0.23 μmol 4-nitrophenol per min per mg of protein and 0.25 mmol 4-nitrophenol β-D-glucopyranoside per L, respectively. The activation energy according to Arrhenius plot was 49.6 KJ/mol.
Registro:
Documento: |
Artículo
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Título: | Factors influencing β-glucosidase production, activity and stability in Nectria catalinensis |
Autor: | Pardo, A.G.; Forchiassin, F. |
Filiación: | Lab. de Micología Exp., Depto. de Ciencias Biológicas, Universidad de Buenos Aires, 1428 - Buenos Aires, Argentina U. de Invest. en Interacciones Biol., Centro de Estudios e Investigations, Universidad Nacional de Quilmes, 1876 - Provincia de Buenos Aires, Argentina
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Palabras clave: | Arrhenius |
Año: | 1999
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Volumen: | 44
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Número: | 1
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Página de inicio: | 71
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Página de fin: | 76
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DOI: |
http://dx.doi.org/10.1007/BF02816225 |
Título revista: | Folia Microbiologica
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Título revista abreviado: | Folia Microbiol.
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ISSN: | 00155632
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CODEN: | FOMIA
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00155632_v44_n1_p71_Pardo |
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Citas:
---------- APA ----------
Pardo, A.G. & Forchiassin, F.
(1999)
. Factors influencing β-glucosidase production, activity and stability in Nectria catalinensis. Folia Microbiologica, 44(1), 71-76.
http://dx.doi.org/10.1007/BF02816225---------- CHICAGO ----------
Pardo, A.G., Forchiassin, F.
"Factors influencing β-glucosidase production, activity and stability in Nectria catalinensis"
. Folia Microbiologica 44, no. 1
(1999) : 71-76.
http://dx.doi.org/10.1007/BF02816225---------- MLA ----------
Pardo, A.G., Forchiassin, F.
"Factors influencing β-glucosidase production, activity and stability in Nectria catalinensis"
. Folia Microbiologica, vol. 44, no. 1, 1999, pp. 71-76.
http://dx.doi.org/10.1007/BF02816225---------- VANCOUVER ----------
Pardo, A.G., Forchiassin, F. Factors influencing β-glucosidase production, activity and stability in Nectria catalinensis. Folia Microbiol. 1999;44(1):71-76.
http://dx.doi.org/10.1007/BF02816225