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Abstract:

The influence of different cultivation conditions on β-glucosidase production and of some parameters on the activity and stability of this enzyme were studied in Nectria catalinensis. Maximal β-glucosidase production was achieved with ammonium nitrate (0.5 g N/L) as nitrogen source. Tween 80, Tween 20 and Triton X-100 increased β-glucosidase yields, Tween 80 was the most effective for enzyme release and growth at a concentration of 3.4 mmol/L. On the other hand, Tween 20 and Triton X-100 had an inhibitory effect on N. catalinensis growth. A temperature of 23 °C and an initial pH of cultures of 6.5 were optimal for biomass and β-glucosidase production. Under optimal cultural conditions (ammonium nitrate, 0.5 g N/L; Tween 80, 3.4 mmol/L; 23 °C; initial pH 6.5) the β-glucosidase yield was increased more than five fold respect to the initial state. Optimal temperature for β-glucosidase activity was 45 °C, the initial activity dropped 60 % after 6 h of incubation at this temperature. Optimal PH for enzyme activity was 5.3. At this pH the β-glucosidase was completely stable after 3 d of incubation The V and Km values calculated from Lineweaver-Burk and Eadie-Hofstee plots were 0.23 μmol 4-nitrophenol per min per mg of protein and 0.25 mmol 4-nitrophenol β-D-glucopyranoside per L, respectively. The activation energy according to Arrhenius plot was 49.6 KJ/mol.

Registro:

Documento: Artículo
Título:Factors influencing β-glucosidase production, activity and stability in Nectria catalinensis
Autor:Pardo, A.G.; Forchiassin, F.
Filiación:Lab. de Micología Exp., Depto. de Ciencias Biológicas, Universidad de Buenos Aires, 1428 - Buenos Aires, Argentina
U. de Invest. en Interacciones Biol., Centro de Estudios e Investigations, Universidad Nacional de Quilmes, 1876 - Provincia de Buenos Aires, Argentina
Palabras clave:Arrhenius
Año:1999
Volumen:44
Número:1
Página de inicio:71
Página de fin:76
DOI: http://dx.doi.org/10.1007/BF02816225
Título revista:Folia Microbiologica
Título revista abreviado:Folia Microbiol.
ISSN:00155632
CODEN:FOMIA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00155632_v44_n1_p71_Pardo

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Citas:

---------- APA ----------
Pardo, A.G. & Forchiassin, F. (1999) . Factors influencing β-glucosidase production, activity and stability in Nectria catalinensis. Folia Microbiologica, 44(1), 71-76.
http://dx.doi.org/10.1007/BF02816225
---------- CHICAGO ----------
Pardo, A.G., Forchiassin, F. "Factors influencing β-glucosidase production, activity and stability in Nectria catalinensis" . Folia Microbiologica 44, no. 1 (1999) : 71-76.
http://dx.doi.org/10.1007/BF02816225
---------- MLA ----------
Pardo, A.G., Forchiassin, F. "Factors influencing β-glucosidase production, activity and stability in Nectria catalinensis" . Folia Microbiologica, vol. 44, no. 1, 1999, pp. 71-76.
http://dx.doi.org/10.1007/BF02816225
---------- VANCOUVER ----------
Pardo, A.G., Forchiassin, F. Factors influencing β-glucosidase production, activity and stability in Nectria catalinensis. Folia Microbiol. 1999;44(1):71-76.
http://dx.doi.org/10.1007/BF02816225