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Abstract:

Objective: To investigate the effects of local inhibition of vascular endothelial growth factor A (VEGFA) on proliferation and apoptosis of follicular cells in rat ovaries. To analyze the role of the PI3K/AKT signaling pathway on VEGFA effects. Design: Experimental study. Setting: Research laboratory. Animal(s): Female Sprague Dawley rats, 21 days old, treated with equine chorionic gonadotropin (eCG). Main Outcome Measure(s): Follicular cell proliferation, apoptosis, and activation of the PI3K/AKT signaling pathway after intrabursal injection of a VEGFA inhibitor. Result(s): Inhibition of VEGFA leads to a decrease in the expression of the proliferation marker proliferating cell nuclear antigen (PCNA) in theca and granulosa cells (GC) and an increase in the activation of caspase 3 in antral follicles. Furthermore, we observed a decrease in the phosphorylation of RAC-alpha serine/threonine-protein kinase (AKT) and its target Bcl2 antagonist of cell death (BAD). No differences were found in the levels of kinase insert domain receptor (KDR) protein or in endothelial cell density. Conclusion(s): The VEGFA prevents apoptosis and stimulates proliferation of follicular cells, regulating follicular growth and development. The PI3K/AKT signaling pathway is one of the pathways involved in this mechanism. Therefore, VEGFA has a role as an antiapoptotic and proliferative factor in follicular cells from the rat ovary. © 2010 American Society for Reproductive Medicine.

Registro:

Documento: Artículo
Título:Intrabursal injection of vascular endothelial growth factor trap in eCG-treated prepubertal rats inhibits proliferation and increases apoptosis of follicular cells involving the PI3K/AKT signaling pathway
Autor:Abramovich, D.; Irusta, G.; Parborell, F.; Tesone, M.
Filiación:Instituto de Biología y Medicina Experimental (IByME)-CONICET, Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:angiogenesis; apoptosis; Ovary; PI3K/AKT; proliferation; VEGFA; caspase 3; chorionic gonadotropin; cycline; phosphatidylinositol 3 kinase; protein bcl 2; protein kinase B; Rac protein; serine; threonine; vasculotropin; vasculotropin receptor 2; animal experiment; animal tissue; apoptosis; article; cell death; cell density; cell growth; cell maturation; cell proliferation; controlled study; endothelium cell; female; gene expression regulation; granulosa cell; inhibition kinetics; nonhuman; ovary follicle cell; prepuberty; priority journal; protein phosphorylation; rat; signal transduction; theca cell; 1-Phosphatidylinositol 3-Kinase; Animals; Apoptosis; bcl-Associated Death Protein; Caspase 3; Cell Proliferation; Endothelial Cells; Enzyme Activation; Female; Gonadotropins, Equine; Injections; Ovarian Follicle; Ovary; Phosphorylation; Proto-Oncogene Proteins c-akt; Rats; Rats, Sprague-Dawley; Receptors, Vascular Endothelial Growth Factor; Recombinant Fusion Proteins; Sexual Maturation; Signal Transduction; Vascular Endothelial Growth Factor A; Vascular Endothelial Growth Factor Receptor-2
Año:2010
Volumen:93
Número:5
Página de inicio:1369
Página de fin:1377
DOI: http://dx.doi.org/10.1016/j.fertnstert.2009.01.127
Título revista:Fertility and Sterility
Título revista abreviado:Fertil. Steril.
ISSN:00150282
CODEN:FESTA
CAS:caspase 3, 169592-56-7; chorionic gonadotropin, 9002-61-3; phosphatidylinositol 3 kinase, 115926-52-8; protein bcl 2, 219306-68-0; protein kinase B, 148640-14-6; serine, 56-45-1, 6898-95-9; threonine, 36676-50-3, 72-19-5; vasculotropin, 127464-60-2; 1-Phosphatidylinositol 3-Kinase, 2.7.1.137; Bad protein, rat; Casp3 protein, rat, 3.4.22.-; Caspase 3, 3.4.22.-; Gonadotropins, Equine; Proto-Oncogene Proteins c-akt, 2.7.11.1; Receptors, Vascular Endothelial Growth Factor, 2.7.10.1; Recombinant Fusion Proteins; VEGF-Trap fusion protein, recombinant; Vascular Endothelial Growth Factor A; Vascular Endothelial Growth Factor Receptor-2, 2.7.10.1; bcl-Associated Death Protein; vascular endothelial growth factor A, rat
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00150282_v93_n5_p1369_Abramovich

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Citas:

---------- APA ----------
Abramovich, D., Irusta, G., Parborell, F. & Tesone, M. (2010) . Intrabursal injection of vascular endothelial growth factor trap in eCG-treated prepubertal rats inhibits proliferation and increases apoptosis of follicular cells involving the PI3K/AKT signaling pathway. Fertility and Sterility, 93(5), 1369-1377.
http://dx.doi.org/10.1016/j.fertnstert.2009.01.127
---------- CHICAGO ----------
Abramovich, D., Irusta, G., Parborell, F., Tesone, M. "Intrabursal injection of vascular endothelial growth factor trap in eCG-treated prepubertal rats inhibits proliferation and increases apoptosis of follicular cells involving the PI3K/AKT signaling pathway" . Fertility and Sterility 93, no. 5 (2010) : 1369-1377.
http://dx.doi.org/10.1016/j.fertnstert.2009.01.127
---------- MLA ----------
Abramovich, D., Irusta, G., Parborell, F., Tesone, M. "Intrabursal injection of vascular endothelial growth factor trap in eCG-treated prepubertal rats inhibits proliferation and increases apoptosis of follicular cells involving the PI3K/AKT signaling pathway" . Fertility and Sterility, vol. 93, no. 5, 2010, pp. 1369-1377.
http://dx.doi.org/10.1016/j.fertnstert.2009.01.127
---------- VANCOUVER ----------
Abramovich, D., Irusta, G., Parborell, F., Tesone, M. Intrabursal injection of vascular endothelial growth factor trap in eCG-treated prepubertal rats inhibits proliferation and increases apoptosis of follicular cells involving the PI3K/AKT signaling pathway. Fertil. Steril. 2010;93(5):1369-1377.
http://dx.doi.org/10.1016/j.fertnstert.2009.01.127