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Abstract:

Objective: To evaluate the immunocontraceptive properties of recombinant DE, a sperm epididymal protein involved in fertilization, via an experimental study in rats as a critical step toward the development of a human immunocontraceptive. Design: In vivo study in rats. Setting: Animal care facility of an academic research center. Animal(s): Seventy-four 90-day-old Wistar male and female rats distributed into three groups. Intervention(s): Animals received five injections (intramuscular and subcutaneous) of recombinant DE (recDE), native DE (nDE), or MBP (maltose-binding protein). At various times, animals were anesthetized and bled. Main Outcome Measure(s): Anti-DE levels and tissue specificity of sera were evaluated by enzyme-linked immunosorbent assay (ELISA) and Western blot, respectively. Fertility was analyzed by natural mating. The testes and epididymides were analyzed by histology. Result(s): Recombinant DE raised an immune response with the same kinetics and higher anti-DE levels than that elicited by nDE. Sera against recDE recognized epitopes of DE that were different from those recognized by anti-nDE sera but specifically reacted with DE in epididymis and sperm without cross-reacting with other tissues tested. Male and female recDE-injected animals presented a statistically significant reduction in their fertility with no evidence of pathologic effects. Conclusion(s): Recombinant DE is able to both elicit a specific immune response and inhibit male and female fertility, supporting the use of this sperm epididymal protein for the development of an immunocontraceptive approach. © 2008 American Society for Reproductive Medicine.

Registro:

Documento: Artículo
Título:Immunocontraceptive properties of recombinant sperm protein DE: implications for the development of novel contraceptives
Autor:Ellerman, D.A.; Busso, D.; Maldera, J.A.; Cuasnicú, P.S.
Filiación:Instituto de Biología y Medicina Experimental (IBYME-CONICET), Vuelta de Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:CRISP; epididymis; fertility; Immunocontraception; sperm; recombinant epididymal protein; recombinant protein; unclassified drug; animal tissue; article; female; fertility; fertilization; immune response; immunization; immunocontraception; in vivo study; male; nonhuman; priority journal; rat; sperm; tissue specificity; Animals; Antibodies; Antibody Formation; Antibody Specificity; Contraception, Immunologic; Contraceptive Agents; Epididymal Secretory Proteins; Female; Fertility; Immunization; Injections, Intramuscular; Injections, Subcutaneous; Kinetics; Male; Membrane Glycoproteins; Rats; Rats, Wistar; Recombinant Proteins
Año:2008
Volumen:89
Número:1
Página de inicio:199
Página de fin:205
DOI: http://dx.doi.org/10.1016/j.fertnstert.2007.02.025
Título revista:Fertility and Sterility
Título revista abreviado:Fertil. Steril.
ISSN:00150282
CODEN:FESTA
CAS:Aeg1 protein, rat; Antibodies; Contraceptive Agents; Epididymal Secretory Proteins; Membrane Glycoproteins; Recombinant Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00150282_v89_n1_p199_Ellerman

Referencias:

  • Ohl, D.A., Naz, R.K., Infertility due to antisperm antibodies (1995) Urology, 46, pp. 591-602
  • Liskin, L., Pile, J., Quillan, W., Vasectomy safe and simple (1983) Popul Rep, 4, pp. 61-100
  • Diekman, A.B., Herr, J.C., Sperm antigens and their use in the development of an immunocontraceptive (1997) Am J Reprod Immunol, 37, pp. 111-117
  • Suri, A., Sperm-based contraceptive vaccines: current status, merits and development (2005) Expert Rev Mol Med, 7, pp. 1-16
  • Naz, R.K., Antisperm immunity for contraception (2006) J Androl, 27, pp. 153-159
  • Cameo, M.S., Blaquier, J.A., Androgen-controlled specific proteins in rat epididymis (1976) J Endocr, 69, pp. 317-324
  • Kohane, A.C., Cameo, M.S., Piñeiro, L., Garberi, J.C., Blaquier, J.A., Distribution and site of production of specific proteins in the rat epididymis (1980) Biol Reprod, 23, pp. 181-187
  • Kohane, A.C., Gonzalez Echeverria, F., Piñeiro, L., Blaquier, J.A., Interaction of proteins of epididymal origin with spermatozoa (1980) Biol Reprod, 23, pp. 737-742
  • Rochwerger, L., Cohen, D.J., Cuasnicú, P.S., Mammalian sperm-egg fusion: the rat egg has complementary sites for a sperm protein that mediates gamete fusion (1992) Dev Biol, 153, pp. 83-90
  • Cohen, D.J., Rochwerger, L., Ellerman, D.A., Morgenfeld, M., Busso, D., Cuasnicu, P.S., Relationship between the association of rat epididymal protein DE with spermatozoa and the behavior and function of the protein (2000) Mol Reprod Dev, 56, pp. 180-188
  • Mizuki, N., Kasahara, M., Mouse submandibular glands express an androgen-regulated transcript encoding an acidic epididymal glycoprotein-like molecule (1992) Mol Cell Endocrinol, 89, pp. 25-32
  • Eberspaecher, U., Roosterman, D., Krätzschmar, J., Haendler, B., Habenicht, U.F., Becker, A., Mouse androgen-dependent epididymal glycoprotein CRISP-1 (DE/AEG): isolation, biochemical characterization, and expression in recombinant form (1995) Mol Reprod Dev, 42, pp. 157-172
  • Hayashi, M., Fujimoto, S., Takano, H., Ushiki, T., Abe, K., Ishikura, H., Characterization of a human glycoprotein with potential role in sperm-egg fusion: cDNA cloning, immunohistochemical localization, and chromosomal assignment of the gene (AEGL1) (1996) Genomics, 32, pp. 367-374
  • Kratzschmar, J., Haendler, B., Eberspaecher, U., Roosterman, D., Donner, P., Schleuning, W.D., The human cysteine-rich secretory protein (CRISP) family. Primary structure and tissue distribution of CRISP-1, CRISP-2 and CRISP-3 (1996) Eur J Biochem, 236, pp. 827-836
  • Cohen, D.J., Ellerman, D.A., Cuasnicú, P.S., Mammalian sperm-egg fusion: evidence that epididymal protein DE plays a role in mouse gamete fusion (2000) Biol Reprod, 63, pp. 462-468
  • Cohen, D.J., Ellerman, D.A., Busso, D., Morgenfeld, M., Piazza, A., Hayashi, M., Evidence that human epididymal protein ARP plays a role in gamete fusion through complementary sites on the surface of the human egg (2001) Biol Reprod, 65, pp. 1000-1005
  • Cuasnicú, P.S., Conesa, D., Rochwerger, L., Potential contraceptive use of an epididymal protein that participates in fertilization (1990) Gamete interaction. Prospects for immunocontraception, pp. 143-153. , Alexander N.J., Griffin D., Spieler J.M., and Waites G.M.H. (Eds), Wiley-Liss, New York
  • Perez Martinez, S., Conesa, D., Cuasnicú, P.S., Potential contraceptive use of epididymal proteins: evidence for the participation of specific antibodies against rat epididymal protein DE in male and female fertility inhibition (1995) J Reprod Immunol, 29, pp. 31-45
  • Ellerman, D.A., Brantua, V.S., Martinez, S.P., Cohen, D.J., Conesa, D., Cuasnicu, P.S., Potential contraceptive use of epididymal proteins: immunization of male rats with epididymal protein DE inhibits sperm fusion ability (1998) Biol Reprod, 59, pp. 1029-1036
  • Ellerman, D.A., Da Ros, V.G., Cohen, D.J., Busso, D., Morgenfeld, M.M., Cuasnicu, P.S., Expression and structure-function analysis of DE, a sperm cysteine-rich secretory protein that mediates gamete fusion (2002) Biol Reprod, 67, pp. 1225-1231
  • Towbin, H., Staehelin, T., Gordon, J., Electrophoretic transfer of proteins from polyacrilamyde gels to nitrocellulose sheets: Procedure and some applications (1979) Proc Natl Acad Sci USA, 76, pp. 4350-4360
  • Martineau, P., Leclerc, C., Hofnung, M., Modulating the immunological properties of a linear B-cell epitope by insertion into permissive sites of the MalE protein (1996) Mol Immunol, 33, pp. 1345-1358
  • Hardy, C.M., Clarke, H.G., Nixon, B., Grigg, J.A., Hinds, L.A., Holland, M.K., Examination of the immunocontraceptive potential of recombinant rabbit fertilin subunits in rabbit (1997) Biol Reprod, 57, pp. 879-886
  • Kasahara, M., Figueroa, F., Klein, J., Molecular cloning of a testis-specific gene from mouse chromosome 17 (1987) Transplant Proc, 19, pp. 815-816
  • Udby, L., Bjartell, A., Malm, J., Egesten, A., Lundwall, A., Cowland, J.B., Characterization and localization of cysteine-rich secretory protein 3 (CRISP-3) in the human male reproductive tract (2005) J Androl, 26, pp. 333-342
  • Tung, K.S.K., Primakoff, P., Woolman-Gamer, L., Myles, D.G., Mechanism of infertility in male guinea pigs immunized with sperm PH-20 (1997) Biol Reprod, 56, pp. 1133-1141

Citas:

---------- APA ----------
Ellerman, D.A., Busso, D., Maldera, J.A. & Cuasnicú, P.S. (2008) . Immunocontraceptive properties of recombinant sperm protein DE: implications for the development of novel contraceptives. Fertility and Sterility, 89(1), 199-205.
http://dx.doi.org/10.1016/j.fertnstert.2007.02.025
---------- CHICAGO ----------
Ellerman, D.A., Busso, D., Maldera, J.A., Cuasnicú, P.S. "Immunocontraceptive properties of recombinant sperm protein DE: implications for the development of novel contraceptives" . Fertility and Sterility 89, no. 1 (2008) : 199-205.
http://dx.doi.org/10.1016/j.fertnstert.2007.02.025
---------- MLA ----------
Ellerman, D.A., Busso, D., Maldera, J.A., Cuasnicú, P.S. "Immunocontraceptive properties of recombinant sperm protein DE: implications for the development of novel contraceptives" . Fertility and Sterility, vol. 89, no. 1, 2008, pp. 199-205.
http://dx.doi.org/10.1016/j.fertnstert.2007.02.025
---------- VANCOUVER ----------
Ellerman, D.A., Busso, D., Maldera, J.A., Cuasnicú, P.S. Immunocontraceptive properties of recombinant sperm protein DE: implications for the development of novel contraceptives. Fertil. Steril. 2008;89(1):199-205.
http://dx.doi.org/10.1016/j.fertnstert.2007.02.025