Navegar

Colección

Datos Estadísticas

Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Objective: To study the effect of letrozole (Let) and anastrozole (Anas) on apoptosis and cell proliferation in epithelial endometrial cells (EEC) from patients with endometriosis (EDT). Design: Prospective study. Setting: Research institute and clinical fertility center. Patient(s): Eighteen women with untreated EDT. Intervention(s): Biopsy specimens of eutopic endometrium were obtained from all subjects. Apoptosis and cell proliferation were examined in EEC after incubation with Let or Anas. Main Outcome Measure(s): Percentage of apoptotic cells (ApC) was evaluated by the acridine orange-ethidium bromide technique; cell proliferation was assessed by 3H-thymidine incorporation. Result(s): Treatment with Let 10 nM and Let 100 nM enhanced values of ApC in cultures from EDT patients. Epithelial endometrial cells treated with Anas 100 nM or Anas 500 nM showed a statistically significant induction on apoptosis levels. Cultures treated with Let 1 nM or Anas 50 nM did not show any significant differences in ApC levels compared with basal conditions. 3H-Thymidine uptake was down regulated by Let 10 nM and Let 100 nM. Similarly, Anas 100 nM and Anas 500 nM showed a significantly lower degree of cell proliferation in EEC. Lower concentrations of Let and Anas did not induce any significant change in cell proliferation rates. Conclusion(s): Our results show that Let and Anas produced a significant and positive effect on apoptosis and cell proliferation on EEC from EDT patients. These findings support the further investigation of aromatase inhibitors as a treatment option in EDT. ©2005 by American Society for Reproductive Medicine.

Registro:

Documento: Artículo
Título:Effects of aromatase inhibitors on proliferation and apoptosis in eutopic endometrial cell cultures from patients with endometriosis
Autor:Meresman, G.F.; Bilotas, M.; Abello, V.; Buquet, R.; Tesone, M.; Sueldo, C.
Filiación:Instituto de Biología Y Medicina Experimental (IBYME), Buenos Aires, Argentina
Departmento de Ginecología, Hospìtal de Clínicas José de San Martín, Buenos Aires, Argentina
Centro de Estudios en Giencología Y Reproducción (CEGyR), Buenos Aires, Argentina
Instituto de Biología y Medicina Experimental, Vuelta de Obligado 2490, Buenos Aires (1428), Argentina
Palabras clave:Apoptosis; Aromatase inhibitors; Cell proliferation; Endometrial epithelial cells; Endometriosis; acridine orange; anastrozole; aromatase inhibitor; ethidium bromide; letrozole; thymidine; tritium; apoptosis; article; biopsy; cell culture; cell isolation; cell proliferation; clinical article; concentration (parameters); controlled study; dose response; down regulation; drug effect; endometriosis; endometrium cell; epithelium cell; female; fertility; health center; human; human cell; human tissue; priority journal; prospective study; statistical significance; Apoptosis; Aromatase Inhibitors; Cells, Cultured; Dose-Response Relationship, Drug; Endometriosis; Endometrium; Female; Humans; Prospective Studies; Statistics, Nonparametric
Año:2005
Volumen:84
Número:2
Página de inicio:459
Página de fin:463
DOI: http://dx.doi.org/10.1016/j.fertnstert.2005.01.137
Título revista:Fertility and Sterility
Título revista abreviado:Fertil. Steril.
ISSN:00150282
CODEN:FESTA
CAS:acridine orange, 494-38-2, 65-61-2; anastrozole, 120511-73-1; ethidium bromide, 1239-45-8; letrozole, 112809-51-5; thymidine, 50-89-5; tritium, 10028-17-8; Aromatase Inhibitors
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00150282_v84_n2_p459_Meresman

Referencias:

  • Sharpe-Timms, K.L., Endometrial anomalies in women with endometriosis (2001) Ann NY Acad Sci, 943, pp. 131-147
  • Meresman, G.F., Vighi, S., Buquet, R.A., Contreras-Ortiz, O., Tesone, M., Rumi, L.S., Apoptosis and expression of Bcl-2 and Bax in eutopic endometrium from women with endometriosis (2000) Fertil Steril, 74, pp. 760-766
  • Meresman, G.F., Auge, L., Baranao, R.I., Lombardi, E., Tesone, M., Sueldo, C., Oral contraceptives suppress cell proliferation and enhance apoptosis of eutopic endometrial tissue from patients with endometriosis (2002) Fertil Steril, 77, pp. 1141-1147
  • Kitawaki, J., Kado, N., Ishihara, H., Koshiba, H., Kitaoka, Y., Honjo, H., Endometriosis: The pathophysiology as an estrogen-dependent disease (2002) J Steroid Biochem Mol Biol, 83, pp. 149-155
  • Bulun, S.E., Fang, Z., Imir, G., Gurates, B., Tamura, M., Yilmaz, B., Aromatase and endometriosis (2004) Semin Reprod Med, 22, pp. 45-50
  • Bulun, S.E., Zeitoun, K.M., Takayama, K., Sasano, H., Estrogen biosynthesis in endometriosis: Molecular basis and clinical relevance (2000) J Mol Endocrinol, 25, pp. 35-42
  • Dheenadayalu, K., Mak, I., Gordts, S., Campo, R., Higham, J., Puttemans, P., Aromatase P450 messenger RNA expression in eutopic endometrium is not a specific marker for pelvic endometriosis (2002) Fertil Steril, 78, pp. 825-829
  • Takayama, K., Zeitoun, K., Gunby, R.T., Sasano, H., Carr, B.R., Bulun, S.E., Treatment of severe postmenopausal endometriosis with an aromatase inhibitor (1998) Fertil Steril, 69, pp. 709-713
  • Ailawadi, R.K., Jobanputra, S., Kataria, M., Gurates, B., Bulun, S.E., Treatment of endometriosis and chronic pelvic pain with letrozole and norethindrone acetate: A pilot study (2004) Fertil Steril, 8, pp. 290-296
  • Revised American Fertility Society classification of endometriosis (1985) Fertil Steril, 43, pp. 351-352. , American Fertility Society
  • Bongso, A., Gajra, B., Lian, N.P., Wong, P.C., Soon-Chye, N., Ratnam, S., Establishment of human endometrial cell cultures (1988) Hum Reprod, 3, pp. 705-713
  • Meresman, G.F., Bilotas, M., Buquet, R.A., Baranao, R.I., Sueldo, C., Tesone, M., Gonadotropin-releasing hormone agonist induces apoptosis and reduces cell proliferation in eutopic endometrial cultures from women with endometriosis (2003) Fertil Steril, 80 (2 SUPPL.), pp. 702-707
  • Meresman, G.F., Barañao, R.I., Tenenbaum, A., Singla, J.J., Neuspiller, N.R., Rumi, L.S., Effect of peritoneal fluid from patients with minimal and severe endometriosis on endometrial stromal cell proliferation (1997) Arch Gynecol Obstet, 259, pp. 109-115
  • Abrams, J.M., White, K., Fessler, L.I., Steller, H., Programmed cell death during Drosophila embryogenesis (1993) Development, 117, pp. 29-43
  • Fazleabas, A.T., Brudney, A., Chai, D., Langoi, D., Bulun, S.E., Steroid receptor and aromatase expression in baboon endometriotic lesions (2003) Fertil Steril, 80 (2 SUPPL.), pp. 820-827
  • Zeitoun, K.M., Bulun, S.E., Aromatase: A key molecule in the pathophysiology of endometriosis and a therapeutic target (1999) Fertil Steril, 72, pp. 961-969
  • Razzi, S., Fava, A., Sartini, A., De Simone, S., Cobellis, L., Petraglia, F., Treatment of severe recurrent endometriosis with an aromatase inhibitor in a young ovariectomised woman (2004) Br J Obstet Gynaecol, 111, pp. 182-184
  • Surrey, E.S., Halme, J., Effect of peritoneal fluid from endometriosis patients on endometrial stromal cell proliferation in vitro (1990) Obstet Gynecol, 76, pp. 792-797
  • Fleming, H., Structure and function of cultured endometrial epithelial cells (1999) Semin Reprod Endocrinol, 17, pp. 93-106
  • Kudoh, M., Susaki, Y., Ideyama, Y., Nanya, T., Mori, M., Shikama, H., Inhibitory effects of a novel aromatase inhibitor, YM511, on growth of endometrial explants and insulin-like growth factor-I gene expression in rats with experimental endometriosis (1997) J Steroid Biochem Mol Biol, 63, pp. 75-80
  • Fang, Z., Yang, S., Gurates, B., Tamura, M., Simpson, E., Evans, D., Genetic or enzymatic disruption of aromatase inhibits the growth of ectopic uterine tissue (2002) J Clin Endocrinol Metab, 87, pp. 3460-3466
  • Thiantanawat, A., Long, B.J., Brodie, A.M., Signaling pathways of apoptosis activated by aromatase inhibitors and antiestrogens (2003) Cancer Res, 63, pp. 8037-8050
  • Mitropoulou, T.N., Tzanakakis, G.N., Kletsas, D., Kalofonos, H.P., Karamanos, N.K., Letrozole as a potent inhibitor of cell proliferation and expression of metalloproteinases (MMP-2 and MMP-9) by human epithelial breast cancer cells (2003) Int J Cancer, 104, pp. 155-160

Citas:

---------- APA ----------
Meresman, G.F., Bilotas, M., Abello, V., Buquet, R., Tesone, M. & Sueldo, C. (2005) . Effects of aromatase inhibitors on proliferation and apoptosis in eutopic endometrial cell cultures from patients with endometriosis. Fertility and Sterility, 84(2), 459-463.
http://dx.doi.org/10.1016/j.fertnstert.2005.01.137
---------- CHICAGO ----------
Meresman, G.F., Bilotas, M., Abello, V., Buquet, R., Tesone, M., Sueldo, C. "Effects of aromatase inhibitors on proliferation and apoptosis in eutopic endometrial cell cultures from patients with endometriosis" . Fertility and Sterility 84, no. 2 (2005) : 459-463.
http://dx.doi.org/10.1016/j.fertnstert.2005.01.137
---------- MLA ----------
Meresman, G.F., Bilotas, M., Abello, V., Buquet, R., Tesone, M., Sueldo, C. "Effects of aromatase inhibitors on proliferation and apoptosis in eutopic endometrial cell cultures from patients with endometriosis" . Fertility and Sterility, vol. 84, no. 2, 2005, pp. 459-463.
http://dx.doi.org/10.1016/j.fertnstert.2005.01.137
---------- VANCOUVER ----------
Meresman, G.F., Bilotas, M., Abello, V., Buquet, R., Tesone, M., Sueldo, C. Effects of aromatase inhibitors on proliferation and apoptosis in eutopic endometrial cell cultures from patients with endometriosis. Fertil. Steril. 2005;84(2):459-463.
http://dx.doi.org/10.1016/j.fertnstert.2005.01.137