Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Objective: To characterize proacrosin/acrosin interaction with isolated zona pellucida (ZP) components. Design: Prospective study. Setting: Basic research laboratory. Patient(s): Recombinant proteins derived from human proacrosin (Rec-40, Rec-30, Rec-20, Rec-10, and Rec-6) and from human ZP glycoproteins (rec-hZPA, ZPB, and ZPC). Intervention(s): In vitro binding assay developed to assess proacrosin/acrosin-ZP interaction. Main Outcome Measure(s): Zona pellucida glycoprotein binding to proacrosin/acrosin; estimation of binding affinity. Result(s): Of all ZP proteins, rec-hZPA demonstrated the highest binding activity toward acrosin (Rec-30) (rec-hZPB: 42% of rec-hZPA; rec-hZPC: 39% of rec-hZPA; P<.0005). Rec-hZPA interaction was disturbed by dextran sulphate (75% inhibition with 10 μM), fucose (67% inhibition with 1.5 μM), and mannose (69% inhibition with 333 mM). Comparing binding activity of proacrosin with other N-terminal acrosin fragments, Rec-40 showed 2.6-3 times higher levels. Moreover, saturable high affinity binding of Rec-40 to ZP components was observed (Kd: 34 nM for rec-hZPA, 38 nM for rec-hZPB, 63 nM for rec-hZPC). Conclusion(s): The rec-hZPA is the major ZP ligand for human proacrosin/acrosin. The interaction involves mannosyl, fucosyl, and sulfated glycans. Binding sites for rec-hZP would be located both at the N- and C-terminus of proacrosin, revealing a key role of the proenzyme in the interaction. ©2005 by American Society for Reproductive Medicine.

Registro:

Documento: Artículo
Título:Binding of recombinant human proacrosin/acrosin to zona pellucida (ZP) glycoproteins. I. Studies with recombinant human ZPA, ZPB, and ZPC
Autor:Furlong, L.I.; Harris, J.D.; Vazquez-Levin, M.H.
Filiación:Instituto de Biología Y Medicina Experimental-CONICET-UBA, Buenos Aires, Argentina
Zonagen Inc., The Woodlands, TX, United States
Instituto de Biología Y Medicina Experimental-CONICET-UBA, Vuelta de Obligado 2490, 1428, Buenos Aires, Argentina
Palabras clave:Acrosin; Acrosome; Fertilization; Human; Sperm-egg interaction; Spermatozoa; Zona pellucida; acrosin; dextran sulfate; fucose; glycan derivative; glycoprotein; mannose; proacrosin; protein subunit; recombinant protein; amino terminal sequence; article; binding affinity; binding site; carboxy terminal sequence; comparative study; human; nonhuman; priority journal; protein binding; protein function; protein interaction; zona pellucida; Acrosin; Animals; Binding Sites; CHO Cells; Cricetinae; Egg Proteins; Enzyme Precursors; Humans; Male; Membrane Glycoproteins; Prospective Studies; Protein Binding; Receptors, Cell Surface; Recombinant Proteins; Zona Pellucida
Año:2005
Volumen:83
Número:6
Página de inicio:1780
Página de fin:1790
DOI: http://dx.doi.org/10.1016/j.fertnstert.2004.12.042
Título revista:Fertility and Sterility
Título revista abreviado:Fertil. Steril.
ISSN:00150282
CODEN:FESTA
CAS:acrosin, 9068-57-9; dextran sulfate, 9011-18-1, 9042-14-2; fucose, 3615-37-0, 3713-31-3; mannose, 31103-86-3, 3458-28-4; proacrosin, 56379-46-5; Acrosin, EC 3.4.21.10; Egg Proteins; Enzyme Precursors; Membrane Glycoproteins; proacrosin, EC 3.4.21.-; Receptors, Cell Surface; Recombinant Proteins; zona pellucida glycoproteins; ZP4 protein, human
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00150282_v83_n6_p1780_Furlong

Referencias:

  • Wassarman, P.M., Jovine, L., Litscher, E.S., A profile of fertilization in mammals (2001) Nat Cell Biol, 3, pp. 59-64
  • Liu, D.Y., Baker, H.W., Defective sperm-zona pellucida interaction: A major cause of failure of fertilization in clinical in-vitro fertilization (2000) Hum Reprod, 15, pp. 702-708
  • Rankin, T., Dean, J., The zona pellucida: Using molecular genetics to study the mammalian egg coat (2000) Reviews of Reprod, 3, pp. 114-121
  • Bauskin, A.R., Franken, D., Eberspaecher, U., Donner, P., Characterization of human zona pellucida glycoproteins (1999) Mol Hum Reprod, 5, pp. 534-540
  • McLeskey, S.B., Dowds, C., Carballada, R., White, R.R., Saling, P.M., Molecules involved in mammalian sperm-egg interaction (1998) Int Rev Cytol, 177, pp. 57-113
  • Spargo, S.C., Hope, R.M., Evolution and nomenclature of the zona pellucida gene family (2003) Biol Reprod, 68, pp. 358-362
  • Hughes, D.C., Barratt, C.L.R., Identification of the true human orthologue of the mouse Zp1 gene: Evidence for a greater complexity in the mammalian zona pellucida? (1999) Biochim Biophys Acta, 1447, pp. 303-306
  • Bleil, J.D., Wassarman, P.M., Autoradiographic visualization of the mouse egg's sperm receptor bound to sperm (1986) J Cell Biol, 102, pp. 1363-1371
  • Bleil, J.D., Greve, J.M., Wassarman, P.M., Identification of a secondary sperm receptor in the mouse egg zona pellucida: Role in maintenance of binding of acrosome-reacted sperm to eggs (1988) Dev Biol, 128, pp. 376-386
  • Kerr, C.L., Hanna, W.F., Shaper, J.H., Wright, W.W., Characterization of zona pellucida glycoprotein 3 (ZP3) and ZP2 binding sites on acrosome-intact mouse sperm (2002) Biol Reprod, 66, pp. 1585-1595
  • Rankin, T.L., Coleman, J.S., Epifano, O., Hoodbhoy, T., Turner, S.G., Castle, P.E., Fertility and taxon-specific sperm binding persist after replacement of mouse sperm receptors with human homologs (2003) Dev Cell, 5, pp. 33-43
  • Oehninger, S., Acosta, A., Hodgen, G.D., Antagonistic and agonistic properties of saccharide moieties in the hemizona assay (1990) Fertil Steril, 53, pp. 143-149
  • Oehninger, S., Clark, G.F., Acosta, A.A., Hodgen, G.D., Nature of the inhibitory effect of complex saccharide moieties on the tight binding of human spermatozoa to the human zona pellucida (1991) Fertil Steril, 55, pp. 165-169
  • Tesarik, J., Mendoza, C., Ramirez, J.P., Moos, J., Solubilized human zona pellucida competes with a fucosylated neoglycoprotein for binding sites on the human sperm surface (1993) Fertil Steril, 60, pp. 344-350
  • Mori, K., Daitoh, T., Kamada, M., Maeda, N., Maegawa, M., Hirano, K., Blocking of human fertilization by carbohydrates (1993) Hum Reprod, 8, pp. 1729-1732
  • Lucas, H., Bercegeay, S., Le Pendu, J., Jean, M., Mirallie, S., Barriere, P., A fucose-containing epitope potentially involved in gamete interaction on the human zona pellucida (1994) Hum Reprod, 9, pp. 1532-1538
  • Miranda, P.V., Gonzalez-Echeverría, F., Marín-Briggiler, C.I., Brandelli, A., Blaquier, J.A., Tezón, J.G., Glycosidic residues involved in human sperm-zona pellucida binding in vitro (1997) Mol Hum Reprod, 3, pp. 399-404
  • Benoff, S., Carbohydrates and fertilization: An overview (1997) Mol Hum Reprod, 3, pp. 599-637
  • Ozgur, K., Patankar, M.S., Oehninger, S., Clark, G.F., Direct evidence for the involvement of carbohydrate sequences in human sperm-zona pellucida binding (1998) Mol Hum Reprod, 4, pp. 318-324
  • Mori, K., Daitoh, T., Irahara, M., Kamada, M., Aono, T., Significance of D-mannose as a sperm receptor site on the zona pellucida in human fertilization (1989) Am J Obstet Gynecol, 161, pp. 207-211
  • Benoff, S., Cooper, G.W., Hurley, I., Napolitano, B., Rosenfeld, D.L., Scholl, G.M., Human sperm fertilizing potential in vitro is correlated with differential expression of a head-specific mannose-ligand receptor (1993) Fertil Steril, 59, pp. 854-862
  • Chen, J.-S., Doncel, G.F., Alvarez, C., Acosta, A.A., Expression of mannose-binding sites on human spermatozoa and their role in sperm-zona pellucida binding (1995) J Androl, 16, pp. 55-63
  • Harris, J.D., Seid, C.A., Fontenot, G.K., Liu, H.F., Expression and purification of recombinant human zona pellucida proteins (1999) Protein Expr Purif, 16, pp. 298-307
  • Shabanowitz, R.B., O'Rand, M.G., Characterization of the human zona pellucida from fertilized and unfertilized eggs (1988) J Reprod Fertil, 82, pp. 151-161
  • Van Duin, M., Polman, J.E.M., De Breet, I.T.M., Van Ginneken, K., Bunschoten, H., Grootenhuis, A., Recombinant human zona pellucida protein ZP3 produced by Chinese hamster ovary cells induces the human sperm acrosome reaction and promotes sperm-egg fusion (1994) Biol Reprod, 51, pp. 607-617
  • Whitmarsh, A.J., Woolnough, M.J., Moore, H.D.M., Hornby, D.P., Barratt, C.L.R., Biological activity of recombinant human ZP3 produced in vitro: Potential for a sperm function test (1996) Mol Hum Reprod, 2, pp. 911-919
  • Bray, C., Son, J.H., Kumar, P., Harris, J.D., Meizel, S., A role for the human sperm glycine receptor/Cl- channel in the acrosome reaction initiated by recombinant ZP3 (2002) Biol Reprod, 66, pp. 91-97
  • Urch, U.A., Biochemistry and function of acrosin (1991) Elements of Mammalian Fertilization, pp. 233-248. , P. Wassarman CRC Press Boca Raton
  • Yamagata, K., Murayama, K., Okabe, M., Toshimori, K., Nakanishi, T., Kashiwabara, S., Acrosin accelerates the dispersal of sperm acrosomal proteins during acrosome reaction (1998) J Biol Chem, 273, pp. 10470-10474
  • Kennedy, W.P., Kaminski, J.M., Van Der Ven, H.H., Jeyendran, R.S., Reid, D.S., Blackwell, J., A simple, clinical assay to evaluate the acrosin activity of human spermatozoa (1989) J Androl, 10, pp. 221-231
  • Tummon, I.S., Yuzpe, A.A., Daniel, S.A.J., Deutsch, A., Total acrosin activity correlates with fertility potential after fertilization in vitro (1991) Fertil. Steril, 56, pp. 933-938
  • De Jonge, C.J., Tarchala, S.M., Rawlins, R.G., Binor, Z., Radwanska, E., Acrosin activity in human spermatozoa in relation to semen quality and in-vitro fertilization (1993) Hum Reprod, 8, pp. 253-257
  • Sharma, R., Hogg, J., Bromham, D.R., Is spermatozoan acrosin a predictor of fertilization and embryo quality in the human? (1993) Fertil Steril, 60, pp. 881-887
  • Marí, S., Rawe, V., Biancotti, J.C., Charreau, E., Dain, L., Vazquez-Levin, M.H., Biochemical and molecular studies on the proacrosin/acrosin system in patients with unexplained infertility (2003) Fertil Steril, 79 (3 SUPPL.), pp. 1676-1679
  • Peknicova, J., Capkova, J., Geussova, G., Ivanova, M., Mollova, M., Monoclonal antibodies to intra-acrosomal proteins inhibit gamete binding in vitro (2001) Theriogenology, 56, pp. 211-223
  • Howes, E., Pascall, J.C., Engel, W., Jones, R., Interactions between mouse ZP2 glycoprotein and proacrosin: A mechanism for secondary binding of sperm to the zona pellucida during fertilization (2001) J Cell Sci, 114, pp. 4127-4136
  • Töpfer-Petersen, E., Henschen, A., Acrosin shows zona and fucose binding, novel properties for a serine proteinase (1987) FEBS Lett, 226, pp. 38-43
  • Jansen, S., Quigley, M., Reik, W., Jones, R., Analysis of polysulfate-binding domains in porcine proacrosin, a putative zona adhesion protein from mammalian spermatozoa (1995) Int J Dev Biol, 39, pp. 501-510
  • Jansen, S., Jones, R., Jenneckens, I., Marschall, B., Kriegesmann, B., Coadwell, J., Site-directed mutagenesis of boar proacrosin reveals residues involved in binding of zona pellucida glycoproteins (1998) Mol Reprod Dev, 51, pp. 184-192
  • Crosby, J.A., Jones, R., Barros, C., Carvallo, P., Characterization of the functional domains of boar acrosin involved in nonenzymatic binding to homologous zona pellucida glycoproteins (1998) Mol Reprod Dev, 49, pp. 426-434
  • Richardson, R.T., O'Rand, M.G., Site-directed mutagenesis of rabbit proacrosin. Identification of residues involved in zona pellucida binding (1996) J Biol Chem, 271, pp. 24069-24074
  • Furlong, L.I., Hellman, U., Krimer, A., Tezón, J.G., Charreau, E.H., Vazquez-Levin, M.H., Expression of human proacrosin in Escherichia coli and binding to zona pellucida (2000) Biol Reprod, 62, pp. 606-615
  • Töpfer-Petersen, E., Cechová, D., Zona pellucida induces conversion of proacrosin to acrosin (1990) Int J Androl, 13, pp. 190-196
  • Lowry, O.H., Rosenbrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) J Biol Chem, 193, pp. 267-275
  • Marín-Briggiler, C.I., Vazquez-Levin, M.H., Gonzalez-Echeverria, F., Blaquier, J.A., Tezon, J.G., Miranda, P.V., Strontium supports human sperm capacitation but not follicular fluid-induced acrosome reaction (1999) Biol Reprod, 61, pp. 673-680
  • Urch, U.A., Patel, H., The interaction of boar sperm proacrosin with its natural substrate, the zona pellucida, and with polysulfated polysaccharides (1991) Development, 111, pp. 1165-1172
  • Mori, E., Kashiwabara, S., Baba, T., Inagaki, Y., Mori, T., Amino acid sequences of porcine Sp38 and proacrosin required for binding to the zona pellucida (1995) Dev Biol, 168, pp. 575-583
  • Zahn, A., Furlong, L.I., Biancotti, J.C., Ghiringhelli, P.D., Marín-Briggiler, C.I., Vazquez-Levin, M.H., Evaluation of the proacrosin/acrosin system and its mechanism of activation in human sperm extracts (2002) J Reprod Immunol, 54, pp. 43-63
  • Yurewicz, E.C., Sacco, A.G., Gupta, S.K., Xu, N., Gage, D.A., Hetero-oligomerization-dependent binding of pig oocyte zona pellucida glycoproteins ZPB and ZPC to boar sperm membrane vesicles (1998) J Biol Chem, 273, pp. 7488-7494
  • Yonezawa, N., Fukui, Y., Kuno, M., Shinohara, H., Golan, A., Mitsui, S., Molecular cloning of bovine zona pellucida glycoproteins ZPA and ZPB and analysis for sperm-binding component of the zona (2001) Eur J Biochem, 268, pp. 3587-3594
  • Koyama, K., Hasegawa, A., Inoue, M., Isojima, S., Blocking of human sperm-zona interaction by monoclonal antibodies to a glycoprotein family (ZP4) of porcine zona pellucida (1991) Biol Reprod, 45, pp. 727-735
  • Tsubamoto, H., Hasegawa, A., Nakata, Y., Naito, S., Yamasaki, N., Koyama, K., Expression of recombinant human zona pellucida protein 2 and its binding capacity to spermatozoa (1999) Biol Reprod, 61, pp. 1649-1654
  • Rath, A., Choudhury, S., Hasegawa, A., Koyama, K., Gupta, S.K., Antibodies generated in response to plasmid DNA encoding zona pellucida glycoprotein-B inhibit in vitro human sperm-egg binding (2002) Mol Reprod Dev, 62, pp. 525-533
  • Sivapurapu, N., Upadhyay, A., Hasegawa, A., Koyama, K., Gupta, S.K., Native zona pellucida reactivity and in-vitro effect on human sperm-egg binding with antisera against bonnet monkey ZP1 and ZP3 synthetic peptides (2002) J Reprod Immunol, 56, pp. 77-91
  • Maymon, B.B.S., Maymon, R., Ben-Nun, I., Ghetler, Y., Shalgi, R., Skutelsky, E., Distribution of carbohydrates in the zona pellucida of human oocytes (1994) J Reprod Fertil, 102, pp. 81-86
  • Lindsay, L.L., Wieduwilt, M.J., Hedrick, J.L., Oviductin, the Xenopus laevis oviductal protease that processes egg envelope glycoprotein gp43, increases sperm binding to envelopes, and is translated as part of an unusual mosaic protein composed of two protease and several CUB domains (1999) Biol Reprod, 60, pp. 989-995
  • Baba, T., Kashiwabara, S., Watanabe, K., Itoh, H., Michikawa, Y., Kimura, K., Activation and maturation mechanisms of boar acrosin zymogen based on the deduced primary structure (1989) J Biol Chem, 264, pp. 11920-11927
  • Veaute, C., Furlong, L., Bronson, R., Vazquez-Levin, M.H., Antibodies towards proacrosin/acrosin in infertile women (2003) Fertil Steril, 80 (3 SUPPL.), p. 10
  • Kim, K.S., Gerton, G.L., Differential release of soluble and matrix components: Evidence for intermediate states of secretion during spontaneous acrosomal exocytosis in mouse sperm (2003) Dev Biol, 264, pp. 141-152
  • Lefièvre, L., Conner, S.J., Salpekar, A., Olufowobi, O., Ashton, P., Pavlovic, B., Four zona pellucida glycoproteins are expressed in the human (2004) Hum Reprod, 19, pp. 1580-1586

Citas:

---------- APA ----------
Furlong, L.I., Harris, J.D. & Vazquez-Levin, M.H. (2005) . Binding of recombinant human proacrosin/acrosin to zona pellucida (ZP) glycoproteins. I. Studies with recombinant human ZPA, ZPB, and ZPC. Fertility and Sterility, 83(6), 1780-1790.
http://dx.doi.org/10.1016/j.fertnstert.2004.12.042
---------- CHICAGO ----------
Furlong, L.I., Harris, J.D., Vazquez-Levin, M.H. "Binding of recombinant human proacrosin/acrosin to zona pellucida (ZP) glycoproteins. I. Studies with recombinant human ZPA, ZPB, and ZPC" . Fertility and Sterility 83, no. 6 (2005) : 1780-1790.
http://dx.doi.org/10.1016/j.fertnstert.2004.12.042
---------- MLA ----------
Furlong, L.I., Harris, J.D., Vazquez-Levin, M.H. "Binding of recombinant human proacrosin/acrosin to zona pellucida (ZP) glycoproteins. I. Studies with recombinant human ZPA, ZPB, and ZPC" . Fertility and Sterility, vol. 83, no. 6, 2005, pp. 1780-1790.
http://dx.doi.org/10.1016/j.fertnstert.2004.12.042
---------- VANCOUVER ----------
Furlong, L.I., Harris, J.D., Vazquez-Levin, M.H. Binding of recombinant human proacrosin/acrosin to zona pellucida (ZP) glycoproteins. I. Studies with recombinant human ZPA, ZPB, and ZPC. Fertil. Steril. 2005;83(6):1780-1790.
http://dx.doi.org/10.1016/j.fertnstert.2004.12.042