Artículo

Novaro, V.; Pustovrh, C.; Colman-Lerner, A.; Radisky, D.; Lo Nostro, F.; Paz, D.; Jawerbaum, A.; González, E. "Nitric oxide induces gelatinase A (matrix metalloproteinase 2) during rat embryo implantation" (2002) Fertility and Sterility. 78(6):1278-1287
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Abstract:

Objective: To evaluate a reciprocal signaling interaction initiated by embryo-derived nitric oxide (NO) to facilitate implantation by increased production of gelatinase A (matrix metalloproteinase 2, MMP2) in uterine stroma. Design: Experimental animal studies. Setting: Reproductive-physiology research laboratory. Animal(s): Female syngeneic Wistar rats aged 14 weeks. Intervention(s): Vaginal smears to confirm pregnancy. Oviductal ligature to avoid the descent of blastocysts to the uterine lumen. Plasma exudation assays to locate uterine blastocyst implantation sites. Organ cultures treated with NO donors and nitric oxide synthase (NOS) inhibitors. Main Outcome Measure(s): Expression of MMP2 and NO was assessed by Western blot and zymography of tissue extracts and by immunofluorescence of tissue sections. Result(s): An increase in MMP2 activity was found in uterine extracts in early pregnant rats and was concentrated at implantation sites. Immunolocalization experiments showed that inducible NOS was expressed on the surface of the implanting blastocyst adjacent to the uterine epithelium at the sites of increased MMP2 expression. In organ culture experiments, NO donors were found to increase, whereas NOS inhibitors were found to decrease MMP2 activity in uterine tissue sections. Conclusion(s): Blastocyst-derived NO contributes to the production of uterine-derived MMP2, an essential component of implantation and initiation of placentation. © 2002 by American Society for Reproductive Medicine.

Registro:

Documento: Artículo
Título:Nitric oxide induces gelatinase A (matrix metalloproteinase 2) during rat embryo implantation
Autor:Novaro, V.; Pustovrh, C.; Colman-Lerner, A.; Radisky, D.; Lo Nostro, F.; Paz, D.; Jawerbaum, A.; González, E.
Filiación:Ctro. Estud. Farmacol. y Botanicos, Depto. de Fisiol. de la Reproduccion, Buenos Aires, Argentina
Molecular Sciences Institute, Lawrence Berkeley Natl. Laboratory, Berkeley, CA, United States
Lawrence Berkeley Natl. Laboratory, Life Sciences Division, 1 Cyclotron Rd, Berkeley, CA 94720, United States
Fac. de Ciencias Exactas y Naturales, Depto. de Biologia Ciudad Univ., Buenos Aires, Argentina
Palabras clave:Decidualization; Maternal-embryo interaction; Pregnancy; Rat uterus; gelatinase A; nitric oxide; nitric oxide donor; nitric oxide synthase inhibitor; tissue extract; animal experiment; animal tissue; article; assay; blastocyst; controlled study; embryo; endometrium; enzyme activity; female; immunofluorescence test; immunolocalization; nidation; nonhuman; organ culture; pregnancy; priority journal; protein expression; rat; rat strain; uterine tube ligation; uterus; vagina smear; Western blotting; zymography; Animals; Blastocyst; Embryo Implantation; Enzyme Induction; Enzyme Inhibitors; Female; Matrix Metalloproteinase 2; Nitric Oxide; Nitric Oxide Donors; Nitric Oxide Synthase; omega-N-Methylarginine; Organ Culture Techniques; Pregnancy; Rats; Rats, Wistar; Uterus
Año:2002
Volumen:78
Número:6
Página de inicio:1278
Página de fin:1287
DOI: http://dx.doi.org/10.1016/S0015-0282(02)04240-1
Título revista:Fertility and Sterility
Título revista abreviado:Fertil. Steril.
ISSN:00150282
CODEN:FESTA
CAS:Enzyme Inhibitors; Matrix Metalloproteinase 2, EC 3.4.24.24; Nitric Oxide Donors; Nitric Oxide Synthase, EC 1.14.13.39; Nitric Oxide, 10102-43-9; omega-N-Methylarginine, 17035-90-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00150282_v78_n6_p1278_Novaro

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Citas:

---------- APA ----------
Novaro, V., Pustovrh, C., Colman-Lerner, A., Radisky, D., Lo Nostro, F., Paz, D., Jawerbaum, A.,..., González, E. (2002) . Nitric oxide induces gelatinase A (matrix metalloproteinase 2) during rat embryo implantation. Fertility and Sterility, 78(6), 1278-1287.
http://dx.doi.org/10.1016/S0015-0282(02)04240-1
---------- CHICAGO ----------
Novaro, V., Pustovrh, C., Colman-Lerner, A., Radisky, D., Lo Nostro, F., Paz, D., et al. "Nitric oxide induces gelatinase A (matrix metalloproteinase 2) during rat embryo implantation" . Fertility and Sterility 78, no. 6 (2002) : 1278-1287.
http://dx.doi.org/10.1016/S0015-0282(02)04240-1
---------- MLA ----------
Novaro, V., Pustovrh, C., Colman-Lerner, A., Radisky, D., Lo Nostro, F., Paz, D., et al. "Nitric oxide induces gelatinase A (matrix metalloproteinase 2) during rat embryo implantation" . Fertility and Sterility, vol. 78, no. 6, 2002, pp. 1278-1287.
http://dx.doi.org/10.1016/S0015-0282(02)04240-1
---------- VANCOUVER ----------
Novaro, V., Pustovrh, C., Colman-Lerner, A., Radisky, D., Lo Nostro, F., Paz, D., et al. Nitric oxide induces gelatinase A (matrix metalloproteinase 2) during rat embryo implantation. Fertil. Steril. 2002;78(6):1278-1287.
http://dx.doi.org/10.1016/S0015-0282(02)04240-1