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Abstract:

Objective: To determine the effect of human sperm incubation at room temperature (20°C) upon capacitation-related events. Design: Prospective study. Setting: Basic research laboratory. Patient(s): Semen samples were obtained from normozoospermic volunteers. Human follicular fluid (hFF) was collected from women undergoing assisted reproductive treatment.Intervention(s): Spermatozoa were incubated for up to 18 hours at 20°C and/or 37°C. Main Outcome Measure(s): Protein tyrosine phosphorylation patterns, development of hyperactivated motility, and induction of acrosome reaction (AR) in response to hFF. Result(s): Spermatozoa incubated for 18 hours at 20°C showed an array of tyrosine phosphorylated proteins similar to noncapacitated cells. After incubation at 20°C, the percentage of spermatozoa displaying hyperactivated motility and undergoing acrosomal loss in response to hFF was significantly lower when compared with cells kept the same time at 37°C. Conversely, spermatozoa incubated overnight at 37°C could respond to hFF, either at 37°C or 20°C. When preincubation at 20°C was followed by sperm exposure to 37°C, capacitation-related events could be activated. In capacitated cells (16 hours at 37°C), 2-hour incubation at 20°C led to a significant decrease in acrosome reaction inducibility, suggesting sperm decapacitation. Conclusion(s): Human sperm incubation at room temperature does not allow capacitation, although it does not affect hFF-induced acrosome reaction in capacitated cells. The blocking effect is overcome when spermatozoa are exposed to 37°C. Copyright © 2002 American Society for Reproductive Medicine.

Registro:

Documento: Artículo
Título:Effect of incubating human sperm at room temperature on capacitation-related events
Autor:Marín-Briggiler, C.I.; Tezón, J.G.; Miranda, P.V.; Vazquez-Levin, M.H.
Filiación:Instituto de Biología y Medicina Experimental, CONICET-UBA, Buenos Aires, Argentina
Instituto de Biologia y Medicina Experimental, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
Palabras clave:Acrosome reaction; Human follicular fluid; Human spermatozoa; Sperm capacitation; Temperature; phosphoprotein; tyrosine; acrosome; article; controlled study; follicle fluid; human; human cell; male; normal human; priority journal; prospective study; protein phosphorylation; room temperature; sample; sperm; spermatozoon; spermatozoon capacitation; spermatozoon motility; temperature; volunteer; Acrosome Reaction; Biomechanics; Blotting, Western; Culture Media; Electrophoresis, Polyacrylamide Gel; Female; Follicular Fluid; Humans; Male; Phosphorylation; Prospective Studies; Proteins; Sperm Capacitation; Sperm Motility; Spermatozoa; Tyrosine
Año:2002
Volumen:77
Número:2
Página de inicio:252
Página de fin:259
DOI: http://dx.doi.org/10.1016/S0015-0282(01)02982-X
Título revista:Fertility and Sterility
Título revista abreviado:Fertil. Steril.
ISSN:00150282
CODEN:FESTA
CAS:Culture Media; Proteins; Tyrosine, 55520-40-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00150282_v77_n2_p252_MarinBriggiler

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Citas:

---------- APA ----------
Marín-Briggiler, C.I., Tezón, J.G., Miranda, P.V. & Vazquez-Levin, M.H. (2002) . Effect of incubating human sperm at room temperature on capacitation-related events. Fertility and Sterility, 77(2), 252-259.
http://dx.doi.org/10.1016/S0015-0282(01)02982-X
---------- CHICAGO ----------
Marín-Briggiler, C.I., Tezón, J.G., Miranda, P.V., Vazquez-Levin, M.H. "Effect of incubating human sperm at room temperature on capacitation-related events" . Fertility and Sterility 77, no. 2 (2002) : 252-259.
http://dx.doi.org/10.1016/S0015-0282(01)02982-X
---------- MLA ----------
Marín-Briggiler, C.I., Tezón, J.G., Miranda, P.V., Vazquez-Levin, M.H. "Effect of incubating human sperm at room temperature on capacitation-related events" . Fertility and Sterility, vol. 77, no. 2, 2002, pp. 252-259.
http://dx.doi.org/10.1016/S0015-0282(01)02982-X
---------- VANCOUVER ----------
Marín-Briggiler, C.I., Tezón, J.G., Miranda, P.V., Vazquez-Levin, M.H. Effect of incubating human sperm at room temperature on capacitation-related events. Fertil. Steril. 2002;77(2):252-259.
http://dx.doi.org/10.1016/S0015-0282(01)02982-X