Artículo
Abstract:
Objective: To examine the effect of a gonadotropin-releasing hormone agonist (GnRH-a), leuprolide acetate (LA), on human chorionic gonadotropin/luteinizing hormone (LH) receptors content and progesterone (P) and estradiol (E2) production in cultured granulosa or luteal cells. Design: Prospective. Setting: Private Fertility Clinic and National Research Institute. Patients: Twenty patients undergoing in vitro fertilization or gamete intrafallopian transfer programs. Results: Human chorionic gonadotropin/LH receptors in human granulosa cells increased after 48 hours of culture, and LA inhibited such effect. Leuprolide acetate, 1 ng/mL, in the cultures produced an increase in P production. On the contrary, LA inhibited E2 production. Additionally, the in vivo effect of LA (2 μg/rat per 7 days) was studied in corpus luteum of superovulated rats. Luteal cells from LA- treated rats in culture produced lower P than the controls but showed an increase in aromatase activity. Luteal LH receptors declined after 48 hours of culture with LA. Conclusion: The high doses of gonadotropin necessary to induce ovarian hyperstimulation when GnRH-a is administered could be related with an inhibitory effect of these agonists on LH receptors and aromatase activity.
Registro:
| Documento: | Artículo |
| Título: | Effect of a gonadotropin-releasing hormone agonist on luteinizing hormone receptors and steroidogenesis in ovarian cells |
| Autor: | Guerrero, H.E.; Stein, P.; Asch, R.H.; De Fried, E.P.; Tesone, M. |
| Filiación: | Instituto de Biol./Med. Experimental, Obligado 2490, 1428 Buenos Aires, Argentina |
| Palabras clave: | GnRH-a; granulosa cells; LH receptors; luteal cells; steroidogenesis; aromatase; chorionic gonadotropin; estradiol; gonadorelin; gonadorelin agonist; human menopausal gonadotropin; leuprorelin; luteinizing hormone; luteinizing hormone receptor; progesterone; urofollitropin; animal cell; article; binding site; cell culture; clinical article; controlled study; corpus luteum; echography; enzyme activity; female; granulosa cell; human; human cell; luteal cell; nonhuman; priority journal; radioimmunoassay; rat; steroidogenesis |
| Año: | 1993 |
| Volumen: | 59 |
| Número: | 4 |
| Página de inicio: | 803 |
| Página de fin: | 809 |
| Título revista: | Fertility and Sterility |
| Título revista abreviado: | FERTIL. STERIL. |
| ISSN: | 00150282 |
| CODEN: | FESTA |
| CAS: | aromatase, 9039-48-9; chorionic gonadotropin, 9002-61-3; estradiol, 50-28-2; gonadorelin, 33515-09-2, 9034-40-6; human menopausal gonadotropin, 61489-71-2; leuprorelin, 53714-56-0, 74381-53-6; luteinizing hormone, 39341-83-8, 9002-67-9; progesterone, 57-83-0; urofollitropin, 97048-13-0 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00150282_v59_n4_p803_Guerrero |
Citas:
---------- APA ----------
Guerrero, H.E., Stein, P., Asch, R.H., De Fried, E.P. & Tesone, M. (1993) . Effect of a gonadotropin-releasing hormone agonist on luteinizing hormone receptors and steroidogenesis in ovarian cells. Fertility and Sterility, 59(4), 803-809.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00150282_v59_n4_p803_Guerrero [ ]
---------- CHICAGO ----------
Guerrero, H.E., Stein, P., Asch, R.H., De Fried, E.P., Tesone, M. "Effect of a gonadotropin-releasing hormone agonist on luteinizing hormone receptors and steroidogenesis in ovarian cells" . Fertility and Sterility 59, no. 4 (1993) : 803-809.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00150282_v59_n4_p803_Guerrero [ ]
---------- MLA ----------
Guerrero, H.E., Stein, P., Asch, R.H., De Fried, E.P., Tesone, M. "Effect of a gonadotropin-releasing hormone agonist on luteinizing hormone receptors and steroidogenesis in ovarian cells" . Fertility and Sterility, vol. 59, no. 4, 1993, pp. 803-809.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00150282_v59_n4_p803_Guerrero [ ]
---------- VANCOUVER ----------
Guerrero, H.E., Stein, P., Asch, R.H., De Fried, E.P., Tesone, M. Effect of a gonadotropin-releasing hormone agonist on luteinizing hormone receptors and steroidogenesis in ovarian cells. FERTIL. STERIL. 1993;59(4):803-809.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00150282_v59_n4_p803_Guerrero [ ]
