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Abstract:

Activity of the human long interspersed nuclear elements-1 (LINE-1) retrotransposon occurs mainly in early embryonic development and during hippocampal neurogenesis. SOX-11, a transcription factor relevant to neuronal development, has unknown functions in the control of LINE-1 retrotransposon activity during neuronal differentiation. To study the dependence of LINE-1 activity on SOX-11 during neuronal differentiation, we induced differentiation of human SH-SY5Y neuroblastoma cells and adult adipose mesenchymal stem cells (hASCs) to a neuronal fate and found increased LINE-1 activity. We also show that SOX-11 protein binding to the LINE-1 promoter is higher in differentiating neuroblastoma cells, while knock-down of SOX-11 inhibits the induction of LINE-1 transcription in differentiating conditions. These results suggest that activation of LINE-1 retrotransposition during neuronal differentiation is mediated by SOX-11. © 2018 Federation of European Biochemical Societies

Registro:

Documento: Artículo
Título:SOX-11 regulates LINE-1 retrotransposon activity during neuronal differentiation
Autor:Orqueda, A.J.; Gatti, C.R.; Ogara, M.F.; Falzone, T.L.
Filiación:Instituto de Medicina Traslacional e Ingeniería Biomédica (IMTIB), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Hospital Italiano de Buenos Aires e Instituto Universitario del Hospital Italiano, Buenos Aires, Argentina
Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-CONICET-UBA), FCEN, UBA, Buenos Aires, Argentina
Instituto de Biología Celular y Neurociencias (IBCN-CONICET-UBA), Facultad de Medicina, UBA, Buenos Aires, Argentina
Instituto de Biología y Medicina Experimental (IBYME-CONICET), Buenos Aires, Argentina
Palabras clave:human adipose-derived stem cells; LINE-1; neuroblastoma; neuronal differentiation; SOX-11; DNA; LINE1 DNA; transcription factor Sox11; unclassified drug; 5' untranslated region; adipose derived stem cell; adipose mesenchymal stem cell; Article; cell fate; controlled study; DNA sequence; DNA transposition; gene knockdown; genetic transcription; human; human cell; mesenchymal stem cell; nerve cell differentiation; priority journal; protein DNA interaction; retroposon; SH-SY5Y cell line; upregulation
Año:2018
Volumen:592
Número:22
Página de inicio:3708
Página de fin:3719
DOI: http://dx.doi.org/10.1002/1873-3468.13260
Título revista:FEBS Letters
Título revista abreviado:FEBS Lett.
ISSN:00145793
CODEN:FEBLA
CAS:DNA, 9007-49-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v592_n22_p3708_Orqueda

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Citas:

---------- APA ----------
Orqueda, A.J., Gatti, C.R., Ogara, M.F. & Falzone, T.L. (2018) . SOX-11 regulates LINE-1 retrotransposon activity during neuronal differentiation. FEBS Letters, 592(22), 3708-3719.
http://dx.doi.org/10.1002/1873-3468.13260
---------- CHICAGO ----------
Orqueda, A.J., Gatti, C.R., Ogara, M.F., Falzone, T.L. "SOX-11 regulates LINE-1 retrotransposon activity during neuronal differentiation" . FEBS Letters 592, no. 22 (2018) : 3708-3719.
http://dx.doi.org/10.1002/1873-3468.13260
---------- MLA ----------
Orqueda, A.J., Gatti, C.R., Ogara, M.F., Falzone, T.L. "SOX-11 regulates LINE-1 retrotransposon activity during neuronal differentiation" . FEBS Letters, vol. 592, no. 22, 2018, pp. 3708-3719.
http://dx.doi.org/10.1002/1873-3468.13260
---------- VANCOUVER ----------
Orqueda, A.J., Gatti, C.R., Ogara, M.F., Falzone, T.L. SOX-11 regulates LINE-1 retrotransposon activity during neuronal differentiation. FEBS Lett. 2018;592(22):3708-3719.
http://dx.doi.org/10.1002/1873-3468.13260