Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Extrinsic apoptosis is initiated by recognition and clustering of the single-pass transmembrane proteins Fas ligand and Fas expressed at the surface of closely apposed lymphocytes and target cells, respectively. Since Fas-mediated death response was mainly studied with soluble antibodies, the mobility constraints for receptor activation by a membrane embedded agonist is not well understood. We explored this influence by stimulating apoptosis on functionalized supported lipid bilayers, where we quantified agonist mobility by z-scan fluorescence correlation spectroscopy. Using different lipid compositions, we show that the apoptotic response correlates with increased lateral mobility of the agonist in the lipid bilayer. © 2015 Federation of European Biochemical Societies.

Registro:

Documento: Artículo
Título:Agonist mobility on supported lipid bilayers affects Fas mediated death response
Autor:Sánchez, M.F.; Levi, V.; Weidemann, T.; Carrer, D.C.
Filiación:Instituto de Investigación Médica Mercedes y Martín Ferreyra (INIMEC), CONICET, Universidad Nacional de Córdoba, Friuli 2434, CC389, Córdoba, 5000, Argentina
Departamento de Química Biológica, IQUIBICEN, Facultad de Ciencias Exactas, Universidad de Buenos Aires, Ciudad UniversitariaBuenos Aires, Argentina
Max Planck Institute of Biochemistry, Cellular and Molecular Biophysics, Am Klopferspitz 18, Martinsried, 82152, Germany
Palabras clave:Apoptosis; Fas receptor; Fluorescence correlation spectroscopy; Functionalized surface; Supported lipid bilayer; Fas antibody; Fas antigen; Fas ligand; Fas antigen; FAS protein, human; lipid bilayer; agonist mobility; apoptosis; Article; concentration (parameters); controlled study; correlational study; fluorescence correlation spectroscopy; HEK293 cell line; human; human cell; lipid bilayer; lipid composition; membrane fluidity; molecular biology; priority journal; quantitative analysis; supported lipid bilayer; z scan fluorescence correlation spectroscopy; agonists; lipid bilayer; membrane fluidity; metabolism; movement (physiology); Antigens, CD95; Apoptosis; HEK293 Cells; Humans; Lipid Bilayers; Membrane Fluidity; Movement
Año:2015
Volumen:589
Número:23
Página de inicio:3527
Página de fin:3533
DOI: http://dx.doi.org/10.1016/j.febslet.2015.10.009
Título revista:FEBS Letters
Título revista abreviado:FEBS Lett.
ISSN:00145793
CODEN:FEBLA
CAS:Antigens, CD95; FAS protein, human; Lipid Bilayers
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v589_n23_p3527_Sanchez

Referencias:

  • Li-Weber, M., Krammer, P.H., Function and regulation of the CD95 (APO-1/Fas) ligand in the immune system (2003) Semin. Immunol., 15, pp. 145-157
  • Falschlehner, C., Schaefer, U., Walczak, H., Following TRAIL's path in the immune system (2009) Immunology, 127, pp. 145-154
  • Depraetere, V., Golstein, P., Fas and other cell death signaling pathways (1997) Semin. Immunol., 9, pp. 93-107
  • Nagata, S., Apoptosis by death factor (1997) Cell, 88, pp. 355-365
  • Feig, C., Peter, M.E., How apoptosis got the immune system in shape (2007) Eur. J. Immunol., 37, pp. S61-S70
  • Smyth, M.J., Cretney, E., Kelly, J.M., Westwood, J.A., Street, S.E., Yagita, H., Takeda, K., Hayakawa, Y., Activation of NK cell cytotoxicity (2005) Mol. Immunol., 42, pp. 501-510
  • Dustin, M.L., Long, E.O., Cytotoxic immunological synapses (2010) Immunol. Rev., 235, pp. 24-34
  • Golstein, P., Signal transduction. FasL binds preassembled Fas (2000) Science, 288, pp. 2328-2329
  • Peter, M.E., Krammer, P.H., The CD95(APO-1/Fas) DISC and beyond (2003) Cell Death Differ., 10, pp. 26-35
  • Lee, K.H., Feig, C., Tchikov, V., Schickel, R., Hallas, C., Schutze, S., Peter, M.E., Chan, A.C., The role of receptor internalization in CD95 signaling (2006) EMBO J., 25, pp. 1009-1023
  • Mollinedo, F., Gajate, C., Fas/CD95 death receptor and lipid rafts: new targets for apoptosis-directed cancer therapy (2006) Drug Resist. Updates, 9, pp. 51-73
  • Hueber, A.O., Bernard, A.M., Herincs, Z., Couzinet, A., He, H.T., An essential role for membrane rafts in the initiation of Fas/CD95-triggered cell death in mouse thymocytes (2002) EMBO Rep., 3, pp. 190-196
  • Cahuzac, N., Baum, W., Kirkin, V., Conchonaud, F., Wawrezinieck, L., Marguet, D., Janssen, O., Hueber, A.O., Fas ligand is localized to membrane rafts, where it displays increased cell death-inducing activity (2006) Blood, 107, pp. 2384-2391
  • Gajate, C., Mollinedo, F., Cytoskeleton-mediated death receptor and ligand concentration in lipid rafts forms apoptosis-promoting clusters in cancer chemotherapy (2005) J. Biol. Chem., 280, pp. 11641-11647
  • Cremesti, A., Paris, F., Grassme, H., Holler, N., Tschopp, J., Fuks, Z., Gulbins, E., Kolesnick, R., Ceramide enables fas to cap and kill (2001) J. Biol. Chem., 276, pp. 23954-23961
  • Grassme, H., Cremesti, A., Kolesnick, R., Gulbins, E., Ceramide-mediated clustering is required for CD95-DISC formation (2003) Oncogene, 22, pp. 5457-5470
  • Carrer, D.C., Maggio, B., Phase behavior and molecular interactions in mixtures of ceramide with dipalmitoylphosphatidylcholine (1999) J. Lipid Res., 40, pp. 1978-1989
  • Lavrik, I.N., Golks, A., Riess, D., Bentele, M., Eils, R., Krammer, P.H., Analysis of CD95 threshold signaling: triggering of CD95 (FAS/APO-1) at low concentrations primarily results in survival signaling (2007) J. Biol. Chem., 282, pp. 13664-13671
  • Ranjan, K., Surolia, A., Pathak, C., Apoptotic potential of Fas-associated death domain on regulation of cell death regulatory protein cFLIP and death receptor mediated apoptosis in HEK 293T cells (2012) J. Cell Commun. Signal., 6, pp. 155-168
  • Zhang, L., Kaizuka, Y., Hanagata, N., Imaging of Fas-FasL membrane microdomains during apoptosis in a reconstituted cell-cell junction (2012) Biochem. Biophys. Res. Commun., 422, pp. 298-304
  • Carrer, D.C., Kummer, E., Chwastek, G., Chiantia, S., Schwille, P., Asymmetry determines the effects of natural ceramides on model membranes (2009) Soft Matter, 5, pp. 3279-3286
  • Richter, R.P., Berat, R., Brisson, A.R., Formation of solid-supported lipid bilayers: an integrated view (2006) Langmuir, 22, pp. 3497-3505
  • Sui, S.F., Urumow, T., Sackmann, E., Interaction of insulin receptors with lipid bilayers and specific and nonspecific binding of insulin to supported membranes (1988) Biochemistry, 27, pp. 7463-7469
  • Machan, R., Hof, M., Lipid diffusion in planar membranes investigated by fluorescence correlation spectroscopy (2010) Biochim. Biophys. Acta, 1798, pp. 1377-1391
  • Garcia-Saez, A.J., Carrer, D.C., Schwille, P., Fluorescence correlation spectroscopy for the study of membrane dynamics and organization in giant unilamellar vesicles (2010) Methods Mol. Biol., 606, pp. 493-508
  • Benda, A., Benes, M., Marecek, V., Lhotsky, A., Hermens, W.T., Hof, M., How to determine diffusion coefficients in planar phospholipid systems by confocal fluorescence correlation spectroscopy (2003) Langmuir, 19, pp. 4120-4126
  • Ganguly, S., Chattopadhyay, A., Cholesterol depletion mimics the effect of cytoskeletal destabilization on membrane dynamics of the serotonin1A receptor: a zFCS study (2010) Biophys. J., 99, pp. 1397-1407
  • Humpolickova, J., Gielen, E., Benda, A., Fagulova, V., Vercammen, J., Vandeven, M., Hof, M., Engelborghs, Y., Probing diffusion laws within cellular membranes by Z-scan fluorescence correlation spectroscopy (2006) Biophys. J., 91, pp. L23-L25
  • Miszta, A., Machan, R., Benda, A., Ouellette, A.J., Hermens, W.T., Hof, M., Combination of ellipsometry, laser scanning microscopy and Z-scan fluorescence correlation spectroscopy elucidating interaction of cryptdin-4 with supported phospholipid bilayers (2008) J. Pept. Sci., 14, pp. 503-509
  • Stefl, M., Kulakowska, A., Hof, M., Simultaneous characterization of lateral lipid and prothrombin diffusion coefficients by z-scan fluorescence correlation spectroscopy (2009) Biophys. J., 97, pp. L01-L03
  • Kahya, N., Scherfeld, D., Bacia, K., Schwille, P., Lipid domain formation and dynamics in giant unilamellar vesicles explored by fluorescence correlation spectroscopy (2004) J. Struct. Biol., 147, pp. 77-89
  • Bacia, K., Scherfeld, D., Kahya, N., Schwille, P., Fluorescence correlation spectroscopy relates rafts in model and native membranes (2004) Biophys. J., 87, pp. 1034-1043
  • Przybylo, M., Sykora, J., Humpolickova, J., Benda, A., Zan, A., Hof, M., Lipid diffusion in giant unilamellar vesicles is more than 2 times faster than in supported phospholipid bilayers under identical conditions (2006) Langmuir, 22, pp. 9096-9099
  • Kahya, N., Schwille, P., How phospholipid-cholesterol interactions modulate lipid lateral diffusion, as revealed by fluorescence correlation spectroscopy (2006) J. Fluoresc., 16, pp. 671-678
  • Kam, L., Boxer, S.G., Cell adhesion to protein-micropatterned-supported lipid bilayer membranes (2001) J. Biomed. Mater. Res., 55, pp. 487-495
  • Groves, J.T., Mahal, L.K., Bertozzi, C.R., Control of cell adhesion and growth with micropatterned supported lipid membranes (2001) Langmuir, 17, pp. 5129-5133
  • Hu, T., Shi, J., Jiao, X., Zhou, J., Yin, X., Measurement of annexin V uptake and lactadherin labeling for the quantification of apoptosis in adherent Tca8113 and ACC-2 cells (2008) Braz. J. Med. Biol. Res., 41, pp. 750-757
  • Koopman, G., Reutelingsperger, C.P., Kuijten, G.A., Keehnen, R.M., Pals, S.T., Van Oers, M.H., Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis (1994) Blood, 84, pp. 1415-1420
  • Kahya, N., Scherfeld, D., Bacia, K., Poolman, B., Schwille, P., Probing lipid mobility of raft-exhibiting model membranes by fluorescence correlation spectroscopy (2003) J. Biol. Chem., 278, pp. 28109-28115
  • Kischkel, F.C., Hellbardt, S., Behrmann, I., Germer, M., Pawlita, M., Krammer, P.H., Peter, M.E., Cytotoxicity-dependent APO-1 (Fas/CD95)-associated proteins form a death-inducing signaling complex (DISC) with the receptor (1995) EMBO J., 14, pp. 5579-5588
  • Neumann, L., Pforr, C., Beaudouin, J., Pappa, A., Fricker, N., Krammer, P.H., Lavrik, I.N., Eils, R., Dynamics within the CD95 death-inducing signaling complex decide life and death of cells (2010) Mol. Syst. Biol., 6, p. 352
  • Hsu, C.J., Hsieh, W.T., Waldman, A., Clarke, F., Huseby, E.S., Burkhardt, J.K., Baumgart, T., Ligand mobility modulates immunological synapse formation and T cell activation (2012) PLoS One, 7
  • Ketchum, C., Miller, H., Song, W., Upadhyaya, A., Ligand mobility regulates B cell receptor clustering and signaling activation (2014) Biophys J., 106, pp. 26-36

Citas:

---------- APA ----------
Sánchez, M.F., Levi, V., Weidemann, T. & Carrer, D.C. (2015) . Agonist mobility on supported lipid bilayers affects Fas mediated death response. FEBS Letters, 589(23), 3527-3533.
http://dx.doi.org/10.1016/j.febslet.2015.10.009
---------- CHICAGO ----------
Sánchez, M.F., Levi, V., Weidemann, T., Carrer, D.C. "Agonist mobility on supported lipid bilayers affects Fas mediated death response" . FEBS Letters 589, no. 23 (2015) : 3527-3533.
http://dx.doi.org/10.1016/j.febslet.2015.10.009
---------- MLA ----------
Sánchez, M.F., Levi, V., Weidemann, T., Carrer, D.C. "Agonist mobility on supported lipid bilayers affects Fas mediated death response" . FEBS Letters, vol. 589, no. 23, 2015, pp. 3527-3533.
http://dx.doi.org/10.1016/j.febslet.2015.10.009
---------- VANCOUVER ----------
Sánchez, M.F., Levi, V., Weidemann, T., Carrer, D.C. Agonist mobility on supported lipid bilayers affects Fas mediated death response. FEBS Lett. 2015;589(23):3527-3533.
http://dx.doi.org/10.1016/j.febslet.2015.10.009