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Pollination includes processes where water and/or solute movements must be finely regulated, suggesting participation of aquaporins. Using information available from different transcriptional profilings of Arabidopsis thaliana mature pollen, we showed that the only aquaporins that are selectively and highly expressed in mature pollen are two TIPs: AtTIP1;3 and AtTIP5;1. Pollen exhibited a lower number and more exclusive type of aquaporin expressed genes when compared to other single cell transcriptional profilings. When characterized using Xenopus oocyte swelling assays, AtTIP1;3 and AtTIP5;1 showed intermediate water permeabilities. Although they displayed neither glycerol nor boric acid permeability they both transported urea. In conclusion, these results suggest a function for AtTIP1;3 and AtTIP5;1 as specific water and urea channels in Arabidopsis pollen. © 2008 Federation of European Biochemical Societies.


Documento: Artículo
Título:AtTIP1;3 and AtTIP5;1, the only highly expressed Arabidopsis pollen-specific aquaporins, transport water and urea
Autor:Soto, G.; Alleva, K.; Mazzella, M.A.; Amodeo, G.; Muschietti, J.P.
Filiación:Instituto de Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Vuelta de Obligado 2490 Piso 2, C1428ADN Buenos Aires, Argentina
Laboratorio de Biomembranas, Departamento de Fisiología, Facultad de Medicina, Buenos Aires, Argentina
Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Arabidopsis; Pollen hydration; Pollen tube; Solute transport; Tonoplast aquaporin; Urea; aquaporin; boric acid; glycerol; urea; animal cell; Arabidopsis; article; cell swelling; controlled study; DNA fingerprinting; gene locus; genetic transcription; nonhuman; oocyte; pollen; priority journal; protein expression; water permeability; water transport; Animals; Aquaporins; Arabidopsis; Arabidopsis Proteins; Biological Transport; Gene Expression Regulation, Plant; Pollen; Transcription, Genetic; Urea; Water; Xenopus; Arabidopsis; Arabidopsis thaliana
Página de inicio:4077
Página de fin:4082
Título revista:FEBS Letters
Título revista abreviado:FEBS Lett.
CAS:aquaporin, 215587-75-0; boric acid, 10043-35-3, 11113-50-1, 11129-12-7, 14213-97-9; glycerol, 56-81-5; urea, 57-13-6; Aquaporins; Arabidopsis Proteins; TIP1;3 protein, Arabidopsis; TIP5;1 protein, Arabidopsis; Urea, 57-13-6; Water, 7732-18-5


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---------- APA ----------
Soto, G., Alleva, K., Mazzella, M.A., Amodeo, G. & Muschietti, J.P. (2008) . AtTIP1;3 and AtTIP5;1, the only highly expressed Arabidopsis pollen-specific aquaporins, transport water and urea. FEBS Letters, 582(29), 4077-4082.
---------- CHICAGO ----------
Soto, G., Alleva, K., Mazzella, M.A., Amodeo, G., Muschietti, J.P. "AtTIP1;3 and AtTIP5;1, the only highly expressed Arabidopsis pollen-specific aquaporins, transport water and urea" . FEBS Letters 582, no. 29 (2008) : 4077-4082.
---------- MLA ----------
Soto, G., Alleva, K., Mazzella, M.A., Amodeo, G., Muschietti, J.P. "AtTIP1;3 and AtTIP5;1, the only highly expressed Arabidopsis pollen-specific aquaporins, transport water and urea" . FEBS Letters, vol. 582, no. 29, 2008, pp. 4077-4082.
---------- VANCOUVER ----------
Soto, G., Alleva, K., Mazzella, M.A., Amodeo, G., Muschietti, J.P. AtTIP1;3 and AtTIP5;1, the only highly expressed Arabidopsis pollen-specific aquaporins, transport water and urea. FEBS Lett. 2008;582(29):4077-4082.