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Abstract:

Members of group I KT-HAK-KUP transporters play an important role in K+ acquisition by plant roots, a process that is strongly affected by salt stress. A PCR-based random mutagenesis approach on HvHAK1 allowed identification of V366I and R591C substitutions, which confer enhanced K+-capture, and improved NaCl, LiCl and NH4Cl tolerance, to yeast cells. Improved K+-capture was linked to an enhanced Vmax. Results reveal an intrinsic protective effect of K+, and assign an important role to the 8th transmembrane domain, as well as the C-terminus, in determining the maximum capacity for the transport of K+ in KT-HAK-KUP transporters. © 2008 Federation of European Biochemical Societies.

Registro:

Documento: Artículo
Título:Point mutations in the barley HvHAK1 potassium transporter lead to improved K+-nutrition and enhanced resistance to salt stress
Autor:Mangano, S.; Silberstein, S.; Santa-María, G.E.
Filiación:Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto Tecnológico de Chascomús, Camino Circunvalación km 6, Chascomus, Provincia de Buenos Aires 7130, Argentina
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Intendente Güiraldes 2160, Ciudad Autonoma de Buenos Aires, 1428, Argentina
Palabras clave:HAK; KUP; Potassium; Sodium; Transporter; ammonium chloride; lithium chloride; sodium chloride; amino acid substitution; article; barley; mutagenesis; nonhuman; nutrition; point mutation; polymerase chain reaction; potassium transport; priority journal; protein domain; salt stress; yeast cell; Amino Acid Substitution; Arginine; Cation Transport Proteins; Cysteine; Ion Transport; Isoleucine; Osmotic Pressure; Plant Proteins; Point Mutation; Potassium; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Salinity; Sodium; Sodium Chloride; Valine; Hordeum
Año:2008
Volumen:582
Número:28
Página de inicio:3922
Página de fin:3928
DOI: http://dx.doi.org/10.1016/j.febslet.2008.10.036
Título revista:FEBS Letters
Título revista abreviado:FEBS Lett.
ISSN:00145793
CODEN:FEBLA
CAS:ammonium chloride, 12125-02-9; lithium chloride, 7447-41-8; sodium chloride, 7647-14-5; Arginine, 74-79-3; Cation Transport Proteins; Cysteine, 52-90-4; HAK2 protein, Hordeum vulgare; Isoleucine, 73-32-5; Plant Proteins; Potassium, 7440-09-7; Saccharomyces cerevisiae Proteins; Sodium Chloride, 7647-14-5; Sodium, 7440-23-5; TRK1 protein, S cerevisiae, 136956-54-2; Valine, 7004-03-7
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v582_n28_p3922_Mangano

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Citas:

---------- APA ----------
Mangano, S., Silberstein, S. & Santa-María, G.E. (2008) . Point mutations in the barley HvHAK1 potassium transporter lead to improved K+-nutrition and enhanced resistance to salt stress. FEBS Letters, 582(28), 3922-3928.
http://dx.doi.org/10.1016/j.febslet.2008.10.036
---------- CHICAGO ----------
Mangano, S., Silberstein, S., Santa-María, G.E. "Point mutations in the barley HvHAK1 potassium transporter lead to improved K+-nutrition and enhanced resistance to salt stress" . FEBS Letters 582, no. 28 (2008) : 3922-3928.
http://dx.doi.org/10.1016/j.febslet.2008.10.036
---------- MLA ----------
Mangano, S., Silberstein, S., Santa-María, G.E. "Point mutations in the barley HvHAK1 potassium transporter lead to improved K+-nutrition and enhanced resistance to salt stress" . FEBS Letters, vol. 582, no. 28, 2008, pp. 3922-3928.
http://dx.doi.org/10.1016/j.febslet.2008.10.036
---------- VANCOUVER ----------
Mangano, S., Silberstein, S., Santa-María, G.E. Point mutations in the barley HvHAK1 potassium transporter lead to improved K+-nutrition and enhanced resistance to salt stress. FEBS Lett. 2008;582(28):3922-3928.
http://dx.doi.org/10.1016/j.febslet.2008.10.036