Abstract:
L-Arginine plays an essential role in the energetic metabolism of Trypanosoma cruzi. In this work we propose a relationship between L-arginine uptake, arginine kinase activity and the parasite replication ability. In epimastigote cultures L-arginine uptake decreases continuously accompanying a cell replication rate reduction. The use of conditioned or fresh medium mimics uptake variations. Interestingly, in non-replicative trypomastigote cells, L-arginine uptake was undetectable. The association between L-arginine uptake and cell replication was demonstrated using the antimitotic agent hydroxyurea. Arginine kinase, the enzyme responsible for phosphoarginine and ATP synthesis, also shows a differential activity in epimastigote and trypomastigote parasite stages. © 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
Registro:
Documento: |
Artículo
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Título: | Arginine metabolism in Trypanosoma cruzi is coupled to parasite stage and replication |
Autor: | Pereira, C.A.; Alonso, G.D.; Ivaldi, S.; Silber, A.; Alves, M.J.M.; Bouvier, L.A.; Flawiá, M.M.; Torres, H.N. |
Filiación: | Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad de Buenos Aires, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina Laboratório de Bioquímica de Parasitas, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil
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Palabras clave: | Arginine kinase; Arginine transport; Arginine uptake; Phosphoarginine; Trypanosoma cruzi; adenosine triphosphate; antimitotic agent; arginine; arginine kinase; hydroxyurea; amino acid metabolism; article; cell division; enzyme activity; epimastigote; life cycle; nonhuman; polyacrylamide gel electrophoresis; priority journal; synthesis; Trypanosoma cruzi; trypomastigote; Western blotting; Animals; Arginine; Arginine Kinase; Biological Transport; Life Cycle Stages; Trypanosoma cruzi; Trypanosoma; Trypanosoma cruzi |
Año: | 2002
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Volumen: | 526
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Número: | 1-3
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Página de inicio: | 111
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Página de fin: | 114
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DOI: |
http://dx.doi.org/10.1016/S0014-5793(02)03157-5 |
Título revista: | FEBS Letters
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Título revista abreviado: | FEBS Lett.
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ISSN: | 00145793
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CODEN: | FEBLA
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CAS: | Arginine Kinase, EC 2.7.3.3; Arginine, 74-79-3
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v526_n1-3_p111_Pereira |
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Citas:
---------- APA ----------
Pereira, C.A., Alonso, G.D., Ivaldi, S., Silber, A., Alves, M.J.M., Bouvier, L.A., Flawiá, M.M.,..., Torres, H.N.
(2002)
. Arginine metabolism in Trypanosoma cruzi is coupled to parasite stage and replication. FEBS Letters, 526(1-3), 111-114.
http://dx.doi.org/10.1016/S0014-5793(02)03157-5---------- CHICAGO ----------
Pereira, C.A., Alonso, G.D., Ivaldi, S., Silber, A., Alves, M.J.M., Bouvier, L.A., et al.
"Arginine metabolism in Trypanosoma cruzi is coupled to parasite stage and replication"
. FEBS Letters 526, no. 1-3
(2002) : 111-114.
http://dx.doi.org/10.1016/S0014-5793(02)03157-5---------- MLA ----------
Pereira, C.A., Alonso, G.D., Ivaldi, S., Silber, A., Alves, M.J.M., Bouvier, L.A., et al.
"Arginine metabolism in Trypanosoma cruzi is coupled to parasite stage and replication"
. FEBS Letters, vol. 526, no. 1-3, 2002, pp. 111-114.
http://dx.doi.org/10.1016/S0014-5793(02)03157-5---------- VANCOUVER ----------
Pereira, C.A., Alonso, G.D., Ivaldi, S., Silber, A., Alves, M.J.M., Bouvier, L.A., et al. Arginine metabolism in Trypanosoma cruzi is coupled to parasite stage and replication. FEBS Lett. 2002;526(1-3):111-114.
http://dx.doi.org/10.1016/S0014-5793(02)03157-5