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Abstract:

Trypanosomatid parasites containing a metabolically unstable ornithine decarboxylase (ODC) are naturally resistant to high levels of α-difluoromethylornithine (DFMO) because this ODC inhibitor, though causing a drastic reduction of intracellular putrescine, elicits only a moderate decrease of the spermidine endogenous pool. In this study we have used a combination of DFMO with cyclohexylamine (CHA; bis-cyclohexylammonium sulfate), an inhibitor of spermidine synthase, to reach a more complete depletion of spermidine. Under these conditions we have observed the arrest of proliferation not only in trypanosomatids with stable ODC but also in parasites with an enzyme of high turnover rate. In all cases the reinitiation of proliferation occurred only after the addition of exogenous spermidine, and neither putrescine nor spermine were able to induce the same effect. © 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Spermidine is essential for normal proliferation of trypanosomatid protozoa
Autor:González, N.S.; Huber, A.; Algranati, I.D.
Filiación:Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires and CONICET, A. Machado 151, 1405 Buenos Aires, Argentina
Palabras clave:Cyclohexylamine; Polyamine; Spermidine; Spermidine synthase; Trypanosomatid proliferation; eflornithine; eticyclidine; ornithine decarboxylase; putrescine; spermidine; article; cell proliferation; controlled study; Crithidia fasciculata; enzyme stability; nonhuman; priority journal; protozoon; Trypanosoma cruzi; Animals; Crithidia fasciculata; Cyclohexylamines; Eflornithine; Enzyme Inhibitors; Ornithine Decarboxylase; Putrescine; Spermidine; Spermidine Synthase; Spermine; Trypanosoma cruzi; Protozoa; Trypanosomatidae
Año:2001
Volumen:508
Número:3
Página de inicio:323
Página de fin:326
DOI: http://dx.doi.org/10.1016/S0014-5793(01)03091-5
Título revista:FEBS Letters
Título revista abreviado:FEBS Lett.
ISSN:00145793
CODEN:FEBLA
CAS:Cyclohexylamines; Eflornithine, 70052-12-9; Enzyme Inhibitors; Ornithine Decarboxylase, EC 4.1.1.17; Putrescine, 110-60-1; Spermidine Synthase, EC 2.5.1.16; Spermidine, 124-20-9; Spermine, 71-44-3
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00145793_v508_n3_p323_Gonzalez.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v508_n3_p323_Gonzalez

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Citas:

---------- APA ----------
González, N.S., Huber, A. & Algranati, I.D. (2001) . Spermidine is essential for normal proliferation of trypanosomatid protozoa. FEBS Letters, 508(3), 323-326.
http://dx.doi.org/10.1016/S0014-5793(01)03091-5
---------- CHICAGO ----------
González, N.S., Huber, A., Algranati, I.D. "Spermidine is essential for normal proliferation of trypanosomatid protozoa" . FEBS Letters 508, no. 3 (2001) : 323-326.
http://dx.doi.org/10.1016/S0014-5793(01)03091-5
---------- MLA ----------
González, N.S., Huber, A., Algranati, I.D. "Spermidine is essential for normal proliferation of trypanosomatid protozoa" . FEBS Letters, vol. 508, no. 3, 2001, pp. 323-326.
http://dx.doi.org/10.1016/S0014-5793(01)03091-5
---------- VANCOUVER ----------
González, N.S., Huber, A., Algranati, I.D. Spermidine is essential for normal proliferation of trypanosomatid protozoa. FEBS Lett. 2001;508(3):323-326.
http://dx.doi.org/10.1016/S0014-5793(01)03091-5