Abstract:
In epimastigotes of Trypanosoma cruzi, the etiological agent of Chagas' disease, arginine kinase activity increased continuously during the exponential phase of growth. A correlation between growth rate, enzyme-specific activity and enzyme protein was observed. Arginine kinase-specific activity, expressed as a function of enzyme protein, remains roughly constant up to 18 days of culture. In the whole range of the culture time mRNA levels showed minor changes indicating that the enzyme activity is post-transcriptionally regulated. Arginine kinase could be proposed as a modulator of energetic reserves under starvation stress condition. © 2001 Federation of European Biochemical Societies.
Registro:
Documento: |
Artículo
|
Título: | Arginine kinase of the flagellate protozoa Trypanosoma cruzi: Regulation of its expression and catalytic activity |
Autor: | Alonso, G.D.; Pereira, C.A.; Remedi, M.S.; Paveto, M.Cristina; Cochella, L.; Ivaldi, M.Soledad; Gerez de Burgos, N.M.; Torres, H.N.; Flawiá, M.M. |
Filiación: | Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nac. de Invest. Cie. y Tec., Univ. de Buenos Aires, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina Cátedra de Química Biológica, Facultad de Medicina. Universidad Nacional de Córdoba, Córdoba, Argentina
|
Palabras clave: | Arginine kinase; Guanidino kinase; Phosphagen; Phosphoarginine; Trypanosoma cruzi; arginine kinase; article; energy expenditure; enzyme activity; enzyme specificity; nonhuman; priority journal; protein expression; starvation; Trypanosoma cruzi; Animals; Arginine Kinase; Cell Division; Culture Media; Gene Expression Regulation, Enzymologic; Trypanosoma cruzi; Mastigophora (flagellates); Protozoa; Trypanosoma; Trypanosoma cruzi |
Año: | 2001
|
Volumen: | 498
|
Número: | 1
|
Página de inicio: | 22
|
Página de fin: | 25
|
DOI: |
http://dx.doi.org/10.1016/S0014-5793(01)02473-5 |
Título revista: | FEBS Letters
|
Título revista abreviado: | FEBS Lett.
|
ISSN: | 00145793
|
CODEN: | FEBLA
|
CAS: | Arginine Kinase, EC 2.7.3.3; Culture Media
|
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v498_n1_p22_Alonso |
Referencias:
- Hird, F., (1986) Comp. Biochem. Physiol., 85 B, pp. 285-288
- Huennekens, F.M., Whiteley, H.R., (1960) Comparative Biochemistry, 1, pp. 107-180. , Phosphoric acid anhydrides and other energy-rich compounds (Florkin, M. and Mason, H.S. Eds.), Academic Press, New York
- Morrison, J.F., (1973) The Enzymes, 8, pp. 457-486. , Arginine kinase and Other Guanidino Kinases (Boyer, P.D., Ed.), Academic Press, New York
- Pereira, C.A., Alonso, G.D., Paveto, C., Iribarren, A., Cabanas, M.L., Torres, H.N., Flawia, M.M., (2000) J. Biol. Chem., 275, pp. 1495-1501
- Brener, Z., (1973) Annu. Rev. Microbiol., 27, pp. 347-382
- Kollien, A.H., Schaub, G.A., (2000) Parasitol. Today, 16, pp. 381-387
- Castellani, O., Ribeiro, L.V., Fernandes, J.F., (1967) J. Protozool., 14, pp. 447-451
- Laemmli, U.K., (1970) Nature, 227, pp. 105-132
- Somogyi, M., (1945) J. Biol. Chem., 160, pp. 61-74
- Nelson, N., (1944) J. Biol. Chem., 153, pp. 375-386
- Bradford, M.M., (1976) Anal. Biochem., 72, pp. 248-254
- Cazzulo, J.J., (1992) FASEB J., 6, pp. 3153-3161
- Adroher, F.J., Osuna, A., Lupiañez, J.A., (1990) Mol. Cell. Biochem., 94, pp. 71-82
- Ter Kuile, B.H., (1997) J. Bacteriol., 179, pp. 4699-4705
- Remedi, M.S., Scaraffia, P., Rodriguez, M., Bronia, D.H., Gerez de Burgos, N.M., (1997) Acta Physiol. Pharmacol. Therap. Latinoam., 47, pp. 64-68
- Sanchez-Moreno, M., Fernandez-Becerra, M.C., Castilla-Calvente, J.J., Osuna, A., (1995) FEMS Microbiol. Lett., 133, pp. 119-125
- Vanhamme, L., Pays, E., (1995) Microbiol. Rev., 59, pp. 223-240
- Myler, P.J., Audleman, L., DeVos, T., Hixson, G., Kiser, P., Lemley, C., Magness, C., Stuart, K., (1999) Proc. Natl. Acad. Sci. USA, 96, pp. 2902-2906
- Teixeira, S.M., Kirchhoff, L.V., Donelson, J.E., (1995) J. Biol. Chem., 270, pp. 22586-22594
Citas:
---------- APA ----------
Alonso, G.D., Pereira, C.A., Remedi, M.S., Paveto, M.Cristina, Cochella, L., Ivaldi, M.Soledad, Gerez de Burgos, N.M.,..., Flawiá, M.M.
(2001)
. Arginine kinase of the flagellate protozoa Trypanosoma cruzi: Regulation of its expression and catalytic activity. FEBS Letters, 498(1), 22-25.
http://dx.doi.org/10.1016/S0014-5793(01)02473-5---------- CHICAGO ----------
Alonso, G.D., Pereira, C.A., Remedi, M.S., Paveto, M.Cristina, Cochella, L., Ivaldi, M.Soledad, et al.
"Arginine kinase of the flagellate protozoa Trypanosoma cruzi: Regulation of its expression and catalytic activity"
. FEBS Letters 498, no. 1
(2001) : 22-25.
http://dx.doi.org/10.1016/S0014-5793(01)02473-5---------- MLA ----------
Alonso, G.D., Pereira, C.A., Remedi, M.S., Paveto, M.Cristina, Cochella, L., Ivaldi, M.Soledad, et al.
"Arginine kinase of the flagellate protozoa Trypanosoma cruzi: Regulation of its expression and catalytic activity"
. FEBS Letters, vol. 498, no. 1, 2001, pp. 22-25.
http://dx.doi.org/10.1016/S0014-5793(01)02473-5---------- VANCOUVER ----------
Alonso, G.D., Pereira, C.A., Remedi, M.S., Paveto, M.Cristina, Cochella, L., Ivaldi, M.Soledad, et al. Arginine kinase of the flagellate protozoa Trypanosoma cruzi: Regulation of its expression and catalytic activity. FEBS Lett. 2001;498(1):22-25.
http://dx.doi.org/10.1016/S0014-5793(01)02473-5