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Werbajh, S.E.; Urtreger, A.J.; Puricelli, L.I.; De Lustig, E.S.; Bal De Kier Joffé, E.; Kornblihtt, A.R. "Downregulation of fibronectin transcription in highly metastatic adenocarcinoma cells" (1998) FEBS Letters. 440(3):277-281
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Abstract:

Silencing of fibronectin (FN) expression seems to be one of the key mechanisms underlying metastatic behaviour. An inverse correlation exists between FN expression levels and the metastatic potential of two related murine mammary adenocarcinomas, M3 and MM3. Primary cultures of M3 tumour, which is moderately metastatic to lung (40% incidence), show a conspicuous FN extracellular matrix (ECM) and high levels of FN mRNA, while primary cultures of the highly metastatic MM3 tumour (95% lung incidence) are negative for FN in immunofluorescence and show at least 40-fold lower levels of FN mRNA, only detectable by RT-PCR, with a different pattern of alternatively spliced EDI isoforms compared to M3 cells. We show that the FN promoter sequence is not altered in MM3 cells. Transfection experiments with CAT constructs indicate that silencing occurs at the transcriptional level, involving the 220-bp proximal promoter region. Copyright (C) 1998 Federation of European Biochemical Societies.

Registro:

Documento: Artículo
Título:Downregulation of fibronectin transcription in highly metastatic adenocarcinoma cells
Autor:Werbajh, S.E.; Urtreger, A.J.; Puricelli, L.I.; De Lustig, E.S.; Bal De Kier Joffé, E.; Kornblihtt, A.R.
Filiación:Lab. de Fisiol. y Biol. Molecular, Depto. de Ciencias Biológicas, Ciudad Universitaria, Pabellón II (1428), Buenos Aires, Argentina
Área Investigación, Inst. de Oncol. Angel H. Roffo, Buenos Aires, Argentina
Palabras clave:Fibronectin; Metastasis; Transcription; fibronectin; messenger RNA; article; breast adenocarcinoma; human; human cell; lung metastasis; metastasis potential; priority journal; promoter region; protein expression; RNA transcription; single strand conformation polymorphism; Murinae
Año:1998
Volumen:440
Número:3
Página de inicio:277
Página de fin:281
DOI: http://dx.doi.org/10.1016/S0014-5793(98)01473-2
Título revista:FEBS Letters
Título revista abreviado:FEBS Lett.
ISSN:00145793
CODEN:FEBLA
CAS:fibronectin, 86088-83-7
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v440_n3_p277_Werbajh

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Citas:

---------- APA ----------
Werbajh, S.E., Urtreger, A.J., Puricelli, L.I., De Lustig, E.S., Bal De Kier Joffé, E. & Kornblihtt, A.R. (1998) . Downregulation of fibronectin transcription in highly metastatic adenocarcinoma cells. FEBS Letters, 440(3), 277-281.
http://dx.doi.org/10.1016/S0014-5793(98)01473-2
---------- CHICAGO ----------
Werbajh, S.E., Urtreger, A.J., Puricelli, L.I., De Lustig, E.S., Bal De Kier Joffé, E., Kornblihtt, A.R. "Downregulation of fibronectin transcription in highly metastatic adenocarcinoma cells" . FEBS Letters 440, no. 3 (1998) : 277-281.
http://dx.doi.org/10.1016/S0014-5793(98)01473-2
---------- MLA ----------
Werbajh, S.E., Urtreger, A.J., Puricelli, L.I., De Lustig, E.S., Bal De Kier Joffé, E., Kornblihtt, A.R. "Downregulation of fibronectin transcription in highly metastatic adenocarcinoma cells" . FEBS Letters, vol. 440, no. 3, 1998, pp. 277-281.
http://dx.doi.org/10.1016/S0014-5793(98)01473-2
---------- VANCOUVER ----------
Werbajh, S.E., Urtreger, A.J., Puricelli, L.I., De Lustig, E.S., Bal De Kier Joffé, E., Kornblihtt, A.R. Downregulation of fibronectin transcription in highly metastatic adenocarcinoma cells. FEBS Lett. 1998;440(3):277-281.
http://dx.doi.org/10.1016/S0014-5793(98)01473-2