Navegar

Colección

Datos Estadísticas

Artículo

El editor solo permite decargar el artículo en su versión post-print desde el repositorio. Por favor, si usted posee dicha versión, enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Cruzipain, the major cysteine proteinase from Trypanosoma cruzi epimastigotes, purified to a sequentially pure form, exists in multiple forms with pI values between 3.7 and 5.1, and an apparent molecular mass of 41 kDa. The enzyme is stable between pH 4.5-9.5. Cruzipain was found to be rapidly and tightly inhibited by various protein inhibitors of the cystatin superfamily (kass = 1.7-79 × 106M-1s-1, Kd = 1.4-72 pM). These results suggest a possible defensive role for the host's cystatins after parasite infection, and may be of use for the design of new therapeutic drugs. © 1995.

Registro:

Documento: Artículo
Título:Inhibition of cruzipain, the major cysteine proteinase of the protozoan parasite, Trypanosoma cruzi, by proteinase inhibitors of the cystatin superfamily
Autor:Stoka, V.; Nycander, M.; Lenarčič, B.; Labriola, C.; Cazzulo, J.J.; Björk, I.; Turk, V.
Filiación:Department of Biochemistry and Molecular Biology, J. Stefan Institute, Jamova 39, 61111 Ljubljana, Slovenia
Department of Veterinary Medical Chemistry, Swedish University of Agricultural Sciences, Uppsala Biomedical Center, Box 575, S-751 23 Uppsala, Sweden
Instituto de Investigaciones Bioquímicas 'Luis F. Leloir', Fundación Campomar-CONICET, Facultad de Ciencias Exactas y Naturales, Antonio Machado 151, 1405 Buenos Aires, Argentina
Palabras clave:Cruzipain; Cystatin; Cysteine proteinase; Inhibition; Kinetics; Trypanosoma cruzi; cruzipain; cystatin; cystatin c; cysteine proteinase; kininogen; proteinase inhibitor; stefin a; stefin b; article; enzyme inhibition; enzyme purification; enzyme structure; ph; priority journal; trypanosoma cruzi; Animal; Chickens; Comparative Study; Cystatins; Cysteine Endopeptidases; Electrophoresis, Polyacrylamide Gel; Human; Isoelectric Focusing; Kinetics; Molecular Weight; Recombinant Proteins; Support, Non-U.S. Gov't; Trypanosoma cruzi
Año:1995
Volumen:370
Número:1-2
Página de inicio:101
Página de fin:104
DOI: http://dx.doi.org/10.1016/0014-5793(95)00798-E
Título revista:FEBS Letters
Título revista abreviado:FEBS Lett.
ISSN:00145793
CODEN:FEBLA
CAS:cystatin, 81989-95-9; cysteine proteinase, 37353-41-6; proteinase inhibitor, 37205-61-1; stefin A, 107544-29-6; stefin B, 106178-19-2; cruzipain, EC 3.4.22.-; Cystatins; Cysteine Endopeptidases, EC 3.4.22; Recombinant Proteins; stefin A, 107544-29-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v370_n1-2_p101_Stoka

Referencias:

  • Brocklehurst, Willenbrock, Salih, (1987) Hydrolytic enzymes, pp. 39-155. , A. neuberger, K. Brocklehurst, Elsevier, Amsterdam
  • Cazzulo, Frasch, (1992) FASEB J., 6, pp. 3259-3264
  • Cazzulo, Hellman, Couso, Parodi, (1990) Mol. Biochem. Parasitol., 38, pp. 41-48
  • Campetella, Martínez, Cazzulo, (1990) FEMS Microbiol. Lett., 67, pp. 145-150
  • Bontempi, Martínez, Cazzulo, (1989) Mol. Biochem. Parasitol., 33, pp. 43-48
  • Martínez, Campetella, Frasch, Cazzulo, (1991) Infect. Immun., 59, pp. 4275-4277
  • Bontempi, Cazzulo, (1990) FEMS Microbiol. Lett., 70, pp. 337-342
  • Souto-Padrón, Campetella, Cazzulo, Souza, Cysteine proteinase in Trypanosoma cruzi: immunocytochemical localization and involvement in parasite-host cell interaction. (1990) J Cell Sci, 96, pp. 485-490
  • Franke de Cazzulo, Martínez, North, Coombs, Cazzulo, (1994) FEMS Microbiol. Lett., 124, pp. 81-86
  • Cazzulo, Couso, Raimondi, Wernstedt, Hellman, (1989) Mol. Biochem. Parasitol., 33, pp. 33-42
  • Cazzulo, Cazzulo Franke, Martínez, Franke de Cazzulo, (1990) Biochim. Biophys. Acta, 1037, pp. 186-191
  • Labriola, Sousa, Cazzulo, (1993) Biol. Res, 26, pp. 101-107
  • Berti, Storer, (1995) J. Mol. Biol., 246, pp. 273-283
  • Cazzulo, Martínez, Parodi, Wernstedt, Hellman, (1992) FEMS Microbiol. Lett., 100, pp. 411-416
  • Hellman, Wernstedt, Cazzulo, (1991) Mol. Biochem. Parasitol., 44, pp. 15-22
  • Campetella, Henriksson, Aslund, Frasch, Petterson, Cazzulo, (1992) Mol. Biochem. Parasitol., 50, pp. 225-234
  • Eakin, McGrath, McKerrow, Fletterick, Craick, (1993) J. Biol. Chem., 268, pp. 6115-6118
  • McGrath, Engel, McKerrow, Craick, Fletterick, (1995) J. Mol. Biol., 247, pp. 251-259
  • Rich, (1986) Proteinase Inhibitors, pp. 153-178. , A.J. Barrett, G. Salvenen, Elsevier, Amsterdam
  • Brzin, Kopitar, Turk, Machleidt, (1983) Hoppe-Seyler's Z. Physiol. Chem., 364, pp. 1475-1480
  • Pol, Olsson, Estrada, Prasthofer, Björk, Characterization by spectroscopic, kinetic and equilibrium methods of the interaction between recombinant human cystatin A (stefin A) and cysteine proteinases. (1995) Biochem J, , in press
  • Jerala, Kroon-Žitko, Turk, (1994) Protein Purif., 5, pp. 65-69
  • Lindahl, Alriksson, Jörnvall, Björk, (1988) Biochemistry, 27, pp. 5074-5082
  • Cimerman, (1993) PhD Thesis, , University of Ljubljana
  • Müller-Esterl, Johnson, Salvenen, Barrett, (1988) Methods Enzymol., 163, pp. 240-256
  • Turk, Križaj, Kralj, Dolenc, Popoviè, Bieth, Turk, (1993) J. Biol. Chem., 268, pp. 7323-7329
  • Gill, von Hippel, (1989) Anal. Biochem., 182, pp. 319-326
  • Turk, Dolenc, Turk, Bieth, (1993) Biochemistry, 32, pp. 375-380
  • Björk, Alriksson, Ylinenjärvi, (1989) Biochemistry, 28, pp. 1568-1573
  • Turk, Čolic, Stoka, Turk, (1994) FEBS Lett., 339, pp. 155-159
  • Martínez, Cazzulo, (1992) FEMS Microbiol. Lett., 95, pp. 225-230
  • Turk, Dolenc, Žerovnik, Turk, Gubenšek, Turk, (1994) Biochemistry, 33, pp. 14800-14806
  • Mbwara, Gumm, Shaw, Lonsdale-Eccles, (1992) Eur. J. Biochem., 204, pp. 371-379
  • Osorio, Pico, Luaces, (1992) Parasitology, 105, pp. 207-210
  • Rockett, Playfair, Ashall, Targett, Angliker, Shaw, (1990) FEBS Lett., 259, pp. 257-259

Citas:

---------- APA ----------
Stoka, V., Nycander, M., Lenarčič, B., Labriola, C., Cazzulo, J.J., Björk, I. & Turk, V. (1995) . Inhibition of cruzipain, the major cysteine proteinase of the protozoan parasite, Trypanosoma cruzi, by proteinase inhibitors of the cystatin superfamily. FEBS Letters, 370(1-2), 101-104.
http://dx.doi.org/10.1016/0014-5793(95)00798-E
---------- CHICAGO ----------
Stoka, V., Nycander, M., Lenarčič, B., Labriola, C., Cazzulo, J.J., Björk, I., et al. "Inhibition of cruzipain, the major cysteine proteinase of the protozoan parasite, Trypanosoma cruzi, by proteinase inhibitors of the cystatin superfamily" . FEBS Letters 370, no. 1-2 (1995) : 101-104.
http://dx.doi.org/10.1016/0014-5793(95)00798-E
---------- MLA ----------
Stoka, V., Nycander, M., Lenarčič, B., Labriola, C., Cazzulo, J.J., Björk, I., et al. "Inhibition of cruzipain, the major cysteine proteinase of the protozoan parasite, Trypanosoma cruzi, by proteinase inhibitors of the cystatin superfamily" . FEBS Letters, vol. 370, no. 1-2, 1995, pp. 101-104.
http://dx.doi.org/10.1016/0014-5793(95)00798-E
---------- VANCOUVER ----------
Stoka, V., Nycander, M., Lenarčič, B., Labriola, C., Cazzulo, J.J., Björk, I., et al. Inhibition of cruzipain, the major cysteine proteinase of the protozoan parasite, Trypanosoma cruzi, by proteinase inhibitors of the cystatin superfamily. FEBS Lett. 1995;370(1-2):101-104.
http://dx.doi.org/10.1016/0014-5793(95)00798-E