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Abstract:

Trypanosoma cruzi epimastigote forms showed a tightly bound Ca2+-calmodulin-dependent protein kinase activity, which could be partially extracted from membranes and axonemes. The enzyme is constituted by subunits which were autophosphorylated in the absence of exogenous substrates. An antibody against CaM kinase II recognized a Ca2+- or Ca2+-CaM-dependent conformational epitope in these fractions. The detected bands were of molecular weights similar to the α and β subunits of the corresponding bovine brain enzyme (60 and 50 kDa). Studies using [125I]CaM revealed the presence of a CaM-binding domain. These experiments confirm that the parasite possesses a paniculate CaM kinase with characteristics similar to the bovine brain enzyme. © 1994.

Registro:

Documento: Artículo
Título:Trypanosoma cruzi epimastigote forms possess a Ca2+-calmodulin dependent protein kinase
Autor:Ogueta, S.B.; Solari, A.; Téllez-Iñón, M.T.
Filiación:Instituto de Investigations en Ingenieria Genética Biologia Molecular (INGEBI), Facultad de Ciencias Exactas Naturales, UBA, Vuelta de Obllgado 2490, 1428 Buenos Aires, Argentina
Facultad de Ciencias Medicos, UBA, Paraguay 2155, 1121 Buenos Aires, Argentina
Palabras clave:Ca2+-calmodulin protein kinase; Cytoskeleton; Flagella; Trypanosoma cruzi; Trypanosomatid; protein kinase (calcium,calmodulin) ii; article; cytoskeleton; epimastigote; flagellum; nonhuman; priority journal; trypanosoma cruzi; Animal; Binding Sites; Brain; Ca(2+)-Calmodulin Dependent Protein Kinase; Calmodulin; Cattle; Cell Fractionation; Cell Membrane; Comparative Study; Cytoskeleton; Flagella; Microscopy, Electron; Support, Non-U.S. Gov't; Trypanosoma cruzi
Año:1994
Volumen:337
Número:3
Página de inicio:293
Página de fin:297
DOI: http://dx.doi.org/10.1016/0014-5793(94)80212-2
Título revista:FEBS Letters
Título revista abreviado:FEBS Lett.
ISSN:00145793
CODEN:FEBLA
CAS:protein kinase (calcium,calmodulin) II, 141467-21-2; Ca(2+)-Calmodulin Dependent Protein Kinase, EC 2.7.1.123; Calmodulin
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v337_n3_p293_Ogueta

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Citas:

---------- APA ----------
Ogueta, S.B., Solari, A. & Téllez-Iñón, M.T. (1994) . Trypanosoma cruzi epimastigote forms possess a Ca2+-calmodulin dependent protein kinase. FEBS Letters, 337(3), 293-297.
http://dx.doi.org/10.1016/0014-5793(94)80212-2
---------- CHICAGO ----------
Ogueta, S.B., Solari, A., Téllez-Iñón, M.T. "Trypanosoma cruzi epimastigote forms possess a Ca2+-calmodulin dependent protein kinase" . FEBS Letters 337, no. 3 (1994) : 293-297.
http://dx.doi.org/10.1016/0014-5793(94)80212-2
---------- MLA ----------
Ogueta, S.B., Solari, A., Téllez-Iñón, M.T. "Trypanosoma cruzi epimastigote forms possess a Ca2+-calmodulin dependent protein kinase" . FEBS Letters, vol. 337, no. 3, 1994, pp. 293-297.
http://dx.doi.org/10.1016/0014-5793(94)80212-2
---------- VANCOUVER ----------
Ogueta, S.B., Solari, A., Téllez-Iñón, M.T. Trypanosoma cruzi epimastigote forms possess a Ca2+-calmodulin dependent protein kinase. FEBS Lett. 1994;337(3):293-297.
http://dx.doi.org/10.1016/0014-5793(94)80212-2