Navegar

Colección

Datos Estadísticas

Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

GTP stimulation of adenylyl cyclase from the dimorphic pathogenic fungus Candida albicans is greatly enhanced by preincubation of membrane proteins with cholera toxin, NAD and ATP. In the presence of [32P]NAD the toxin catalyzes the covalent incorporation of radioactivity into a menbrane protein of 40 kDa. Pertussis toxin catalyzes the transference of the radioactivity from [32P]NAD to a 32 kDa protein. Two major proteins of 40-42 and 30-32 kDa can also be recognized in Western blots by an anti Gα-common antibody. The results support the idea that G proteins are part of the hormonesensory transduction chain of Candida [(1990) Biochem. Biophys. Res. Commun. 167. 1177-1183]. © 1992.

Registro:

Documento: Artículo
Título:Enzymatic and immunological detection of G protein α-subunits in the pathogenic fungus Candida albicans
Autor:Paveto, C.; Montero, L.; Passeron, S.
Filiación:Departamento de Quimica Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buinos Aires, Ciudad Universitaria, Pabellon II, 40 Piso, Buenos Aires, Argentina
Palabras clave:Adenylyl cyclase; Candida albicans; G-protein; Hormone response; Peptide antiserum, α-commons; adenosine triphosphate; adenylate cyclase; cholera toxin; guanine nucleotide binding protein; membrane protein; nicotinamide adenine dinucleotide; pertussis toxin; protein subunit; article; candida albicans; nonhuman; priority journal; signal transduction; Adenosine Triphosphate; Adenylate Cyclase; Blotting, Western; Candida albicans; Cholera Toxin; GTP-Binding Proteins; Guanosine Triphosphate; Membrane Proteins; NAD; Poly(ADP-ribose) Polymerases; Support, Non-U.S. Gov't; Virulence Factors, Bordetella
Año:1992
Volumen:311
Número:1
Página de inicio:51
Página de fin:54
DOI: http://dx.doi.org/10.1016/0014-5793(92)81365-S
Título revista:FEBS Letters
Título revista abreviado:FEBS Lett.
ISSN:00145793
CODEN:FEBLA
CAS:adenosine triphosphate, 15237-44-2, 56-65-5, 987-65-5; adenylate cyclase, 9012-42-4; nicotinamide adenine dinucleotide, 53-84-9; pertussis toxin, 70323-44-3; Adenosine Triphosphate, 56-65-5; Adenylate Cyclase, EC 4.6.1.1; Cholera Toxin, 9012-63-9; GTP-Binding Proteins, EC 3.6.1.-; Guanosine Triphosphate, 86-01-1; Membrane Proteins; NAD, 53-84-9; Poly(ADP-ribose) Polymerases, EC 2.4.2.30; Virulence Factors, Bordetella
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v311_n1_p51_Paveto

Referencias:

  • Nolting, Hagemeier, Fegeler, Effekt von Insulin auf die Keimschlauch-und Myzel-Bildung von Candida albicans: Effect of Insulin on Germ Tube and Mycelial Formation of Candida albicans (1981) Mycoses, 25, pp. 36-41
  • Kinsman, Pitblado, Coulson, (1988) Mycoses, 31, pp. 617-625
  • Willams, Dickinson, Kinsman, Bramley, Menzies, Adams, (1990) J. Gen. Microbiol., 136, pp. 2140-2148
  • Bramley, Menzies, Willams, Adams, Kinsman, (1990) Biochem. Biophys. Res. Commun., 167, pp. 1050-1056
  • Paveto, Egidy, Galvagno, Passeron, (1990) Biochem. Biophys. Res. Commun., 167, pp. 1177-1183
  • Paveto, Mallo, Egidy, Galvagno, Passeron, (1991) Cell Biol. Int. Rep., 15, pp. 169-178
  • Williams, Dickinson, Kinsman, Bramley, Menzies, Adams, (1990) J. Gen. Microbiol., 136, pp. 2143-2148
  • Sadhu, Hoekstra, McEachern, Reed, Hicks, (1992) Mol. Cell Biol., 12, pp. 1977-1985
  • Egidy, Paveto, Passeron, Calvagno, (1989) Exp. Mycol., 13, pp. 428-432
  • Bradford, (1976) Anal. Biochem., 72, pp. 248-254
  • Birmbauer, Codina, Riveiro-Neto, Mattera, Iyengar, (1988) Techniques in the Study of Transmembrane Signal Transduction Regulating cAMP Formation, , W.T. Shrader, B.W. O'Malley, Chapter 9
  • Alvarez, Daniels, (1990) Anal. Blocliern., 187, pp. 98-103
  • Mumby, Kahn, Manning, Gilman, (1986) Proc. Natl. Acad. Sci. USA, 83, pp. 265-269
  • Cassel, Pfeuffer, (1978) Proc. Natl. Acad. Sci. USA, 75, pp. 2669-2673
  • Dietzel, Kurjan, The yeast SCG1 gene: A Gα-like protein implicated in the a- and α-factor response pathway (1987) Cell, 50, pp. 1001-1010
  • Jahng, Ferguson, Reed, (1988) Mol. Cell. Biol., 8, pp. 2484-2493
  • Strathman, Simon, G protein diversity: a distinct class of alpha subunits is present in vertebrates and invertebrates. (1990) Proceedings of the National Academy of Sciences, 87, pp. 9113-9117
  • Toda, Uno, Ishikawa, Powers, Kataoka, Broek, Cameron, Wigler, (1985) Cell, 40, pp. 27-36

Citas:

---------- APA ----------
Paveto, C., Montero, L. & Passeron, S. (1992) . Enzymatic and immunological detection of G protein α-subunits in the pathogenic fungus Candida albicans. FEBS Letters, 311(1), 51-54.
http://dx.doi.org/10.1016/0014-5793(92)81365-S
---------- CHICAGO ----------
Paveto, C., Montero, L., Passeron, S. "Enzymatic and immunological detection of G protein α-subunits in the pathogenic fungus Candida albicans" . FEBS Letters 311, no. 1 (1992) : 51-54.
http://dx.doi.org/10.1016/0014-5793(92)81365-S
---------- MLA ----------
Paveto, C., Montero, L., Passeron, S. "Enzymatic and immunological detection of G protein α-subunits in the pathogenic fungus Candida albicans" . FEBS Letters, vol. 311, no. 1, 1992, pp. 51-54.
http://dx.doi.org/10.1016/0014-5793(92)81365-S
---------- VANCOUVER ----------
Paveto, C., Montero, L., Passeron, S. Enzymatic and immunological detection of G protein α-subunits in the pathogenic fungus Candida albicans. FEBS Lett. 1992;311(1):51-54.
http://dx.doi.org/10.1016/0014-5793(92)81365-S