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Abstract:

Monoclonal antibodies to Neurospora crassa cyclic nucleotide phosphodiesterase (PDE I) were selected by their capacity to inhibit the enzyme activity. The monoclonal immunoglobulin, coupled to Sepharose 4B, was used for the affinity purification of PDE I activity. After SDS-polyacrylamide gel electrophoresis the affinity purified PDE I fractions showed a single polypeptide band of about 41 kDa. This band reacted in Western blots with the above mentioned monoclonal immunoglobulin. © 1988.

Registro:

Documento: Artículo
Título:Cyclic nucleotide phosphodiesterase activity in Neurospora crassa Purification by immunoaffinity chromatography and characterization
Autor:Ulloa, R.M.; Rubinstein, C.P.; Molina y Vedia, L.; Torres, H.N.; Téllez-Iñón, M.T.
Filiación:Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Facultad de Ciencias Exactas y Naturales, Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:(Neurospora crassa); Cyclic AMP phosphodiesterase; Immunoaffinity chromatography; Monoclonal antibody; cyclic amp phosphodiesterase; monoclonal antibody; enzyme purification; fungus; immunoaffinity chromatography; neurospora crassa; nonhuman; priority journal; 3',5'-Cyclic-Nucleotide Phosphodiesterase; Antibodies, Monoclonal; Chromatography, Affinity; Immunoglobulins, Heavy-Chain; Immunoglobulins, Light-Chain; Kinetics; Molecular Weight; Neurospora; Neurospora crassa
Año:1988
Volumen:241
Número:1-2
Página de inicio:219
Página de fin:222
DOI: http://dx.doi.org/10.1016/0014-5793(88)81065-2
Título revista:FEBS Letters
Título revista abreviado:FEBS Lett.
ISSN:00145793
CODEN:FEBLA
CAS:cyclic AMP phosphodiesterase, 9036-21-9; 3',5'-Cyclic-Nucleotide Phosphodiesterase, EC 3.1.4.17; Antibodies, Monoclonal; Immunoglobulins, Heavy-Chain; Immunoglobulins, Light-Chain
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v241_n1-2_p219_Ulloa

Referencias:

  • Téllez-Iñón, Glikin, Torres, Cyclic nucleotide phosphodiesterase activities in Neurospora crassa. (1982) Biochem J, 203, pp. 611-616
  • Ortega-Pérez, Van, Marmé, Cox, Turian, (1981) FEBS Lett., 133, pp. 205-209
  • Téllez-Iñón, Ulloa, Glikin, Torres, (1985) Biochem. J., 232, pp. 425-430
  • Reig, Téllez-Iñón, Flawiá, Torres, (1984) Biochem. J., 221, pp. 541-543
  • Molina, Torruella, Flawiá, Torres, (1983) Biochem. Biophys. Res. Commun., 113, pp. 778-783
  • Torruella, Flawiá, Eisenschlos, Molina, Rubinstein, Torres, (1986) Biochem. J., 234, pp. 145-150
  • Fundenberg, (1967) Methods in Immunology and Immunochemistry, 1, pp. 306-335. , C.A. Williams, M.W. Chase, Academic Press, New York
  • Williams, (1971) Methods in Immunology and Immunochemistry, 3, pp. 234-273. , C.A. Williams, M.W. Chase, Academic Press, New York
  • Garberi, Macario, Conway, (1985) J. Bacteriol., 164, pp. 1-6
  • Laemmli, (1970) Nature, 227, pp. 680-685
  • Towbin, Staphelin, Gordon, (1979) Proc. Natl. Acad. Sci. USA, 76, pp. 4350-4354
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  • Londesborough, (1976) Anal. Biochem., 71, pp. 623-628

Citas:

---------- APA ----------
Ulloa, R.M., Rubinstein, C.P., Molina y Vedia, L., Torres, H.N. & Téllez-Iñón, M.T. (1988) . Cyclic nucleotide phosphodiesterase activity in Neurospora crassa Purification by immunoaffinity chromatography and characterization. FEBS Letters, 241(1-2), 219-222.
http://dx.doi.org/10.1016/0014-5793(88)81065-2
---------- CHICAGO ----------
Ulloa, R.M., Rubinstein, C.P., Molina y Vedia, L., Torres, H.N., Téllez-Iñón, M.T. "Cyclic nucleotide phosphodiesterase activity in Neurospora crassa Purification by immunoaffinity chromatography and characterization" . FEBS Letters 241, no. 1-2 (1988) : 219-222.
http://dx.doi.org/10.1016/0014-5793(88)81065-2
---------- MLA ----------
Ulloa, R.M., Rubinstein, C.P., Molina y Vedia, L., Torres, H.N., Téllez-Iñón, M.T. "Cyclic nucleotide phosphodiesterase activity in Neurospora crassa Purification by immunoaffinity chromatography and characterization" . FEBS Letters, vol. 241, no. 1-2, 1988, pp. 219-222.
http://dx.doi.org/10.1016/0014-5793(88)81065-2
---------- VANCOUVER ----------
Ulloa, R.M., Rubinstein, C.P., Molina y Vedia, L., Torres, H.N., Téllez-Iñón, M.T. Cyclic nucleotide phosphodiesterase activity in Neurospora crassa Purification by immunoaffinity chromatography and characterization. FEBS Lett. 1988;241(1-2):219-222.
http://dx.doi.org/10.1016/0014-5793(88)81065-2