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Abstract:

Treatment of the chemically transformed fibroblasts BP-A31 and other cell lines with low concentrations of cycloheximide (CHM) for 72 h followed by the removal of the protein synthesis inhibitor leads to the proliferation of α-difluoromethylornithine (DFMO)-resistant phenotypes. These drug-resistant cells contain almost no ornithine decarboxylase (ODC) activity and concomitantly very low levels of putrescine and spermidine. Southern blot analysis and measurements of ODC activity and intracellular polyamine levels showed that the described mechanism of inducing resistance to DFMO triggered by CHM does not involve ODC gene amplification, altered transport of the drug or reduced affinity of the enzyme for DFMO. © 1986.

Registro:

Documento: Artículo
Título:A novel mechanism of resistance to α-difluoromethylornithine induced by cycloheximide. Growth with abnormally low levels of putrescine and spermidine
Autor:Medrano, E.E.; Burrone, O.R.; Ferrer, M.M.; Cafferata, E.G.A.; Algranati, I.D.
Filiación:Instituto de Investigaciones Bioquimicas 'Fundacion Campomar', Facultad de Ciencias Exactas y Naturales (UBA), Antonio Machado 151, 1405 Buenos Aires, Argentina
Palabras clave:Cycloheximide; Ornithine decarboxylase; Polyamine; α-Difluoromethylornithine resistance; cycloheximide; eflornithine; putrescine; spermidine; cell line; drug inhibition; drug resistance; fibroblast; in vitro study; nonhuman; priority journal; Animals; Cell Line; Cycloheximide; Drug Resistance; Eflornithine; Fibroblasts; Humans; Mice; Ornithine Decarboxylase; Putrescine; Spermidine
Año:1986
Volumen:206
Número:1
Página de inicio:106
Página de fin:110
DOI: http://dx.doi.org/10.1016/0014-5793(86)81349-7
Título revista:FEBS Letters
Título revista abreviado:FEBS Lett.
ISSN:00145793
CODEN:FEBLA
CAS:cycloheximide, 642-81-9, 66-81-9; eflornithine, 67037-37-0, 70052-12-9; putrescine, 110-60-1, 333-93-7; spermidine, 124-20-9, 334-50-9; Cycloheximide, 66-81-9; Eflornithine, 70052-12-9; Ornithine Decarboxylase, 4.1.1.17; Putrescine, 110-60-1; Spermidine, 124-20-9
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00145793_v206_n1_p106_Medrano.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v206_n1_p106_Medrano

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Citas:

---------- APA ----------
Medrano, E.E., Burrone, O.R., Ferrer, M.M., Cafferata, E.G.A. & Algranati, I.D. (1986) . A novel mechanism of resistance to α-difluoromethylornithine induced by cycloheximide. Growth with abnormally low levels of putrescine and spermidine. FEBS Letters, 206(1), 106-110.
http://dx.doi.org/10.1016/0014-5793(86)81349-7
---------- CHICAGO ----------
Medrano, E.E., Burrone, O.R., Ferrer, M.M., Cafferata, E.G.A., Algranati, I.D. "A novel mechanism of resistance to α-difluoromethylornithine induced by cycloheximide. Growth with abnormally low levels of putrescine and spermidine" . FEBS Letters 206, no. 1 (1986) : 106-110.
http://dx.doi.org/10.1016/0014-5793(86)81349-7
---------- MLA ----------
Medrano, E.E., Burrone, O.R., Ferrer, M.M., Cafferata, E.G.A., Algranati, I.D. "A novel mechanism of resistance to α-difluoromethylornithine induced by cycloheximide. Growth with abnormally low levels of putrescine and spermidine" . FEBS Letters, vol. 206, no. 1, 1986, pp. 106-110.
http://dx.doi.org/10.1016/0014-5793(86)81349-7
---------- VANCOUVER ----------
Medrano, E.E., Burrone, O.R., Ferrer, M.M., Cafferata, E.G.A., Algranati, I.D. A novel mechanism of resistance to α-difluoromethylornithine induced by cycloheximide. Growth with abnormally low levels of putrescine and spermidine. FEBS Lett. 1986;206(1):106-110.
http://dx.doi.org/10.1016/0014-5793(86)81349-7