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Abstract:

Organic solvents miscible in water (cosolvents) exerted a dual effect on the activation stage of two thioredoxin-linked enzymes of the reductive pentose phosphate cycle, phosphoribulokinase and NADP-glyceraldehyde-3-P dehydrogenase, both from spinach chloroplast; the enzyme specific activity was stimulated and inhibited by low and high concentrations of alcohols, respectively. On the contrary, cosolvents inhibited the catalytic process. In the stimulation of phosphoribulokinase activation, organic solvents reduced the requirement for thioredoxin-f and changed the thiol specificity, so that monothiols became functional. The cosolvent-mediated enhancement of NADP-glyceraldehyde-3-P dehydrogenase was obtained in the absence of modulators. With both enzymes, the concentration of the organic solvents required for activation was inversely proportional to its hydrophobicity (1-butanol < 1-propanol < 2-propanol < ethanol). The present results demonstrate the participation of a new component, the enzyme microenvironment, in the regulation of thioredoxin-linked chloroplast enzymes. © 1985.

Registro:

Documento: Artículo
Título:Dual effects of organic solvents on chloroplast phosphoribulokinase and NADP-glyceraldehyde-3-P dehydrogenase
Autor:Wolosiuk, R.A.; Corley, E.; Crawford, N.A.; Buchanan, B.B.
Filiación:Instituto de Investigaciones Bioquimicas, Facultad de Ciencias Exactas y Naturales (UBA), Antonio Machado 151, 1405 Buenos Aires, Argentina
Division of Molecular Plant Biology, University of California, Berkeley, CA 94720, United States
Palabras clave:Chloroplast enzyme; Enzyme activation tPhotosynthesis; NADP-glyceraldehyde-3-phosphate dehydrogenase; Organic solvent; Phosphoribulokinase; organic solvent; phosphoribulokinase; chloroplast; enzyme activation; higher plant; nicotinamide adenine dinucleotide phosphate glyceraldehyde 3 phosphate dehydrogenase; nonhuman; photosynthesis; priority journal
Año:1985
Volumen:189
Número:2
Página de inicio:212
Página de fin:216
DOI: http://dx.doi.org/10.1016/0014-5793(85)81025-5
Título revista:FEBS Letters
Título revista abreviado:FEBS Lett.
ISSN:00145793
CODEN:FEBLA
CAS:phosphoribulokinase, 9030-60-8
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00145793_v189_n2_p212_Wolosiuk.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v189_n2_p212_Wolosiuk

Referencias:

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  • Crawford, N.A., Yee, B.C., Hutchenson, S.W., Wolosiuk, R.A. and Buchanan, B.B., submitted

Citas:

---------- APA ----------
Wolosiuk, R.A., Corley, E., Crawford, N.A. & Buchanan, B.B. (1985) . Dual effects of organic solvents on chloroplast phosphoribulokinase and NADP-glyceraldehyde-3-P dehydrogenase. FEBS Letters, 189(2), 212-216.
http://dx.doi.org/10.1016/0014-5793(85)81025-5
---------- CHICAGO ----------
Wolosiuk, R.A., Corley, E., Crawford, N.A., Buchanan, B.B. "Dual effects of organic solvents on chloroplast phosphoribulokinase and NADP-glyceraldehyde-3-P dehydrogenase" . FEBS Letters 189, no. 2 (1985) : 212-216.
http://dx.doi.org/10.1016/0014-5793(85)81025-5
---------- MLA ----------
Wolosiuk, R.A., Corley, E., Crawford, N.A., Buchanan, B.B. "Dual effects of organic solvents on chloroplast phosphoribulokinase and NADP-glyceraldehyde-3-P dehydrogenase" . FEBS Letters, vol. 189, no. 2, 1985, pp. 212-216.
http://dx.doi.org/10.1016/0014-5793(85)81025-5
---------- VANCOUVER ----------
Wolosiuk, R.A., Corley, E., Crawford, N.A., Buchanan, B.B. Dual effects of organic solvents on chloroplast phosphoribulokinase and NADP-glyceraldehyde-3-P dehydrogenase. FEBS Lett. 1985;189(2):212-216.
http://dx.doi.org/10.1016/0014-5793(85)81025-5