Abstract:
ABUIN, G., COUTO, A. S., DE LEDERKREMER, R. M., CASAL, O. L., GALLI, C., COLLI, W., AND ALVES, M. J. M. 1996. Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor. Experimental Parasitology 82, 290-297. Tc-85, an 85-kDa surface glycoprotein specific for the trypomastigote stage of Trypanosoma cruzi, has been implicated in the invasion of host cells by the parasite. Radioactive palmitic acid was incorporated into Tc-85 immunoprecipitated from the culture medium with the H1A10 monoclonal antibody, suggesting that shedding occurs with Tc-85 bearing its GPI anchor. In contrast to the glycoprotein remaining in the parasites, the glycosylphosphatidylinositol moiety in shed Tc-85 is resistant to phosphatidylinositol phospholipase C and becomes susceptible to the enzyme following alkali treatment. An alkylglycerol was identified by thin layer chromatography of an ether extract after the enzymatic reaction. Resistance to cleavage by phospholipase C is due to fatty acid esterification of the inositol residue in shed Tc-85. This is the first example of inositol modification in anchors from a glycoprotein of Trypanosoma cruzi. © 1996 Academic Press, Inc.
Registro:
Documento: |
Artículo
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Título: | Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor |
Autor: | Abuin, G.; Couto, A.S.; De Lederkremer, R.M.; Casal, O.L.; Galli, C.; Colli, W.; Alves, M.J.M. |
Filiación: | Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Caixa Postal 26077, 05599-970 São Paulo, Brazil Depto. de Quim. Orgánica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, 1428 Buenos Aires, Argentina
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Palabras clave: | Trypanosoma; Trypanosoma cruzi; glycoprotein; inositol; membrane protein; animal experiment; article; controlled study; high performance liquid chromatography; immunoprecipitation; nonhuman; polyacrylamide gel electrophoresis; priority journal; thin layer chromatography; trypanosoma cruzi; trypomastigote; Animals; Antibodies, Monoclonal; Blotting, Western; Chromatography, Thin Layer; Glycoproteins; Glycosylphosphatidylinositols; Membrane Proteins; Precipitin Tests; Protozoan Proteins; Trypanosoma cruzi |
Año: | 1996
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Volumen: | 82
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Número: | 3
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Página de inicio: | 290
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Página de fin: | 297
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DOI: |
http://dx.doi.org/10.1006/expr.1996.0036 |
Título revista: | Experimental Parasitology
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Título revista abreviado: | Exp. Parasitol.
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ISSN: | 00144894
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CODEN: | EXPAA
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CAS: | Antibodies, Monoclonal; Glycoproteins; Glycosylphosphatidylinositols; Membrane Proteins; Protozoan Proteins
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144894_v82_n3_p290_Abuin |
Referencias:
- Abuin, G., Colli, W., De Souza, W., Alves, M.J.M., A surface antigen of Trypanosoma cruzi involved in cell invasion (Tc-85) is heterogeneous in expression and molecular constitution (1989) Molecular and Biochemical Parasitology, 35, pp. 229-238
- Alves, M.J.M., Abuin, G., Kuwajima, V.Y., Colli, W., Partial inhibition of trypomastigotes entry into cultured mammalian cells by monoclonal antibodies against a surface glycoprotein of Trypanosoma cruzi (1986) Molecular and Biochemical Parasitology, 21, pp. 75-82
- Andrews, N.W., Colli, W., Adhesion and interiorization of Trypanosoma cruzi in mammalian cells (1982) Journal of Protozoology, 29, pp. 264-269
- Andrews, N.W., Robbins, E.S., Ley, V., Hong, K.S., Nussensweig, V., Developmentally regulated, phospholipase C-mediated release of the major surface glycoprotein of amastigotes of Trypanosoma cruzi (1988) Journal of Experimental Medicine, 167, pp. 300-314
- Araguth, M.F., Rodrigues, M.M., Yoshida, N., Trypanosoma cruzi metacyclic trypomastigotes: Neutralization by the stage-specific monoclonal antibody 1G7 and immunogenicity of 90 kD surface antigen (1988) Parasite Immunology, 10, pp. 707-712
- Arruda, M.V., Colli, W., Zingales, B., Terminal β-D-galactofuranosyl epitopes recognized by antibodies that inhibit Trypanosoma cruzi internalization into mammalian cells (1989) European Journal of Biochemistry, 182, pp. 413-421
- Bordier, C., Phase separation of integral membrane proteins in Triton X-114 solution (1981) Journal of Biological Chemistry, 256, pp. 1604-1607
- Boschetti, M.A., Piras, M.M., Henriquez, D., Piras, R., The interaction of T. cruzi surface protein with Vero cells and its relationship with parasite adhesion (1987) Molecular and Biochemical Parasitology, 24, pp. 175-184
- Butikofer, P., Kuypers, F.A., Xu, C.M., Chiu, D.T.Y., Lukin, B., Enrichment of two glycosylphosphatidylinositol-anchored proteins, acetylcholinesterase and decay accelerating factor in vesicles released from human red blood cells (1989) Blood, 74, pp. 1481-1485
- Colli, W., Trans-sialidase: A unique enzyme activity discovered in the protozoan Trypanosoma cruzi (1993) FASEB Journal, 7, pp. 1257-1264
- Couto, A.S., Katzin, A.M., Colli, W., Lederkremer, R.M., Sialic acid in a complex oligosaccharide chain of the Tc-85 surface glycoprotein from the trypomastigote stage of Trypanosoma cruzi (1987) Molecular and Biochemical Parasitology, 26, pp. 145-154
- Couto, A.S., Gonçalves, M.F., Colli, W., Lederkremer, R.M., The N-linked carbohydrate chain of the 85 kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3) galactose units (1990) Molecular and Biochemical Parasitology, 39, pp. 101-108
- Couto, A.S., Lederkremer, R.M., Colli, W., Alves, M.J.M., The glycosylphosphatidylinositol anchor of the trypomastigote-specific Tc-85 glycoprotein from Trypanosoma cruzi. Metabolic-labeling and structural studies (1993) European Journal of Biochemistry, 217, pp. 597-602
- Cross, G.A.M., Takle, G.B., The surface trans-sialidase family of Trypanosoma cruzi (1993) Annual Review of Microbiology, 47, pp. 385-411
- Ferguson, M.A.J., Site of palmitoylation of a phospholipase C-resistant glycosylphosphatidylinositol membrane anchor (1992) Biochemical Journal, 284, pp. 297-300
- Gerold, P., Dieckmann-Schuppert, A., Schwarz, R.T., Glycosylphosphatidylonositols synthesized by asexual erythrocytic stages of the malarial parasite, Plasmodium falciparum. Candidates for plasmodial glycosylphosphatidylinositol membrane anchor precursors and pathogenicity factors (1994) Journal of Biological Chemistry, 269, pp. 2597-2606
- Giordano, R., Chammas, R., Veiga, S.S., Colli, W., Alves, M.J.M., An acidic component of the heterogeneous Tc-85 protein family from the surface of Trypanosoma cruzi is a laminin binding glycoprotein (1994) Molecular and Biochemical Parasitology, 65, pp. 85-94
- Gonçalves, M.F., Umezawa, E.S., Katzin, A.M., De Souza, W., Alves, M.J.M., Zingales, B., Colli, W., Trypanosoma cruzi: Shedding of surface antigens as membrane vesicles (1991) Experimental Parasitology, 72, pp. 43-53
- Güther, M.L.S., De Cardoso Almeida, M.L., Yoshida, N., Ferguson, M.A.J., Structural studies on the glycosylphosphatidylinositol membrane anchor of Trypanosoma cruzi 1G7-antigen (1992) Journal of Biological Chemistry, 267, pp. 6820-6828
- Hernandez-Munain, C., Fernandez, M.A., Alcina, A., Fresno, M., Characterization of a glycosylphosphatidylinositol anchored membrane protein from Trypanosoma cruzi (1991) Infection and Immunity, 59, pp. 1409-1416
- Hirose, S., Ravi, L., Prince, G.M., Rosenfeld, M.G., Silber, R., Andresen, S.W., Hazra, S.V., Medof, M.E., Synthesis of mannosylglucosaminylinositol phospholipids in normal but not paroxysmal nocturnal hemoglobinuria cells (1992) Proceedings of the National Academy of Sciences USA, 89, pp. 6025-6029
- Katzin, A.M., Colli, W., Lectin receptors in Trypanosoma cruzi: An N-acetyl-D-glucosamine containing surface glycoprotein specific for the trypomastigote stage (1983) Biochimica et Biophysica Acta, 68, pp. 208-215
- King, D.L., Chang, Y.D., Turco, S., Cell surface lipophosphoglycan of Leishmania donovani (1987) Molecular and Biochemical Parasitology, 24, pp. 47-53
- Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685
- Lederkremer, R.M., Lima, C.E., Ramirez, M.I., Gonçalves, M.F., Colli, W., Hexadecylpalmitoylglycerol or ceramide is linked to similar glycophosphoinositol anchor-like structures in Trypanosoma cruzi (1993) European Journal of Biochemistry, 218, pp. 929-936
- Lima, M.F., Villalta, F., Trypanosoma cruzi trypomastigote clones differentially express a parasite cell adhesion molecule (1989) Molecular and Biochemical Parasitology, 33, pp. 159-170
- Mayor, S., Menon, A.K., Structural analysis of the glycosylinositol phospholipid anchors of membrane proteins (1990) Methods: A Companion to Methods in Enzymology, 1, pp. 297-305
- Mayor, S., Menon, A.K., Cross, G.A.M., Glycolipid precursors for the membrane anchor of Trypanosoma brucei variant surface glycoproteins. II. Lipid structures of phosphatidylinositol-specific phospholipase C sensitive and resistant glycolipids (1990) Journal of Biological Chemistry, 265, pp. 6174-6181
- McConville, M.J.M., Ferguson, M.A.J., The structure, biosynthesis and function of glycosylated phosphatidylinositols in the parasitic protozoa and higher eukaryotes (1993) Biochemical Journal, 294, pp. 305-324
- Ouaissi, M.A., Role of the RGD sequence in parasite adhesion to host cells (1988) Parasitology Today, 46, pp. 169-173
- Pollevick, G.D., Affranchino, J.L., Frasch, A.D.D., Sanchez, D.O., The complete sequence of a shed acute phase antigen of Trypanosoma cruzi (1991) Molecular and Biochemical Parasitology, 47, pp. 247-250
- Puoti, A., Conzelmann, A., Structural characterization of free glycolipids which are potential precursors for glycosylphosphatidylinositol anchors in mouse thymoma cell lines (1992) Journal of Biological Chemistry, 267, pp. 22673-22680
- Richier, P., Arpagaus, M., Toutant, J.P., Glycolipid-anchored acetylcholinesterases from rabbit lymphocytes and erythrocytes differ in their sensitivity to phosphatidylinositol-specific phospholipase C (1992) Biochimica et Biophysica Acta, 1112, pp. 83-88
- Roberts, W.L., Myher, J.J., Kulsis, A., Low, M.G., Rosenberry, T.L., Lipid analysis of the glycoinositolphospholipid membrane anchor of human erythrocyte acetylcholinesterase: Palmitoylation of inositol results in resistance to phosphatidylinositol-specific phospholipase C (1988) Journal of Biological Chemistry, 263, pp. 18766-18775
- Rosenberg, I., Prioli, R.P., Ortega-Barria, E., Pereira, M.E.A., Stage-specific phospholipase C-mediated release of Trypanosoma cruzi neuraminidase (1991) Molecular and Biochemical Parasitology, 46, pp. 303-306
- Schenkman, S., Yoshida, N., De Cardoso Almeida, M.L., Glycophosphatidylinositol-anchored proteins in metacyclic trypomastigotes of Trypanosoma cruzi (1988) Molecular and Biochemical Parasitology, 29, pp. 141-152
- Silva, L.H.P., Nussenzweig, V., Sobre uma cepa de Trypanosoma cruzi altamente virulenta para o camundongo branco (1953) Folia Clinica et Biologica, 20, pp. 191-207
- Towbin, H., Staehlin, T., Gordon, J., Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications (1979) Proceedings of the National Academy of Sciences USA, 76, pp. 4350-4354
- Urakaze, M., Kamitani, T., Degasperi, R., Sugiyama, E., Chang, H.-M., Warren, C.D., Yeh, E.T.H., Identification of a missing link in glycosylphosphatidylinositol anchor biosynthesis in mammalian cells (1992) Journal of Biological Chemistry, 267, pp. 6459-6462
- Wong, Y.W., Low, M.G., Biosynthesis of glycosylphosphatidylinositol-anchored human placental alkaline phosphatase: Evidence for a phospholipase C-sensitive precursor and its post-attachment conversion into a phospholipase C-resistant form (1994) Biochemical Journal, 301, pp. 205-209
- Zamze, S.E., Ferguson, M.A.J., Collins, R., Dwek, R.A., Rademacher, T.W., Characterization of the cross reacting determinant (CRD) of the glycosylphosphatidylinositol membrane anchor of Trypanosoma brucei variant surface glycoprotein (1988) European Journal of Biochemistry, 176, pp. 527-534
Citas:
---------- APA ----------
Abuin, G., Couto, A.S., De Lederkremer, R.M., Casal, O.L., Galli, C., Colli, W. & Alves, M.J.M.
(1996)
. Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor. Experimental Parasitology, 82(3), 290-297.
http://dx.doi.org/10.1006/expr.1996.0036---------- CHICAGO ----------
Abuin, G., Couto, A.S., De Lederkremer, R.M., Casal, O.L., Galli, C., Colli, W., et al.
"Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor"
. Experimental Parasitology 82, no. 3
(1996) : 290-297.
http://dx.doi.org/10.1006/expr.1996.0036---------- MLA ----------
Abuin, G., Couto, A.S., De Lederkremer, R.M., Casal, O.L., Galli, C., Colli, W., et al.
"Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor"
. Experimental Parasitology, vol. 82, no. 3, 1996, pp. 290-297.
http://dx.doi.org/10.1006/expr.1996.0036---------- VANCOUVER ----------
Abuin, G., Couto, A.S., De Lederkremer, R.M., Casal, O.L., Galli, C., Colli, W., et al. Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor. Exp. Parasitol. 1996;82(3):290-297.
http://dx.doi.org/10.1006/expr.1996.0036