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Abstract:

ABUIN, G., COUTO, A. S., DE LEDERKREMER, R. M., CASAL, O. L., GALLI, C., COLLI, W., AND ALVES, M. J. M. 1996. Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor. Experimental Parasitology 82, 290-297. Tc-85, an 85-kDa surface glycoprotein specific for the trypomastigote stage of Trypanosoma cruzi, has been implicated in the invasion of host cells by the parasite. Radioactive palmitic acid was incorporated into Tc-85 immunoprecipitated from the culture medium with the H1A10 monoclonal antibody, suggesting that shedding occurs with Tc-85 bearing its GPI anchor. In contrast to the glycoprotein remaining in the parasites, the glycosylphosphatidylinositol moiety in shed Tc-85 is resistant to phosphatidylinositol phospholipase C and becomes susceptible to the enzyme following alkali treatment. An alkylglycerol was identified by thin layer chromatography of an ether extract after the enzymatic reaction. Resistance to cleavage by phospholipase C is due to fatty acid esterification of the inositol residue in shed Tc-85. This is the first example of inositol modification in anchors from a glycoprotein of Trypanosoma cruzi. © 1996 Academic Press, Inc.

Registro:

Documento: Artículo
Título:Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor
Autor:Abuin, G.; Couto, A.S.; De Lederkremer, R.M.; Casal, O.L.; Galli, C.; Colli, W.; Alves, M.J.M.
Filiación:Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Caixa Postal 26077, 05599-970 São Paulo, Brazil
Depto. de Quim. Orgánica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, 1428 Buenos Aires, Argentina
Palabras clave:Trypanosoma; Trypanosoma cruzi; glycoprotein; inositol; membrane protein; animal experiment; article; controlled study; high performance liquid chromatography; immunoprecipitation; nonhuman; polyacrylamide gel electrophoresis; priority journal; thin layer chromatography; trypanosoma cruzi; trypomastigote; Animals; Antibodies, Monoclonal; Blotting, Western; Chromatography, Thin Layer; Glycoproteins; Glycosylphosphatidylinositols; Membrane Proteins; Precipitin Tests; Protozoan Proteins; Trypanosoma cruzi
Año:1996
Volumen:82
Número:3
Página de inicio:290
Página de fin:297
DOI: http://dx.doi.org/10.1006/expr.1996.0036
Título revista:Experimental Parasitology
Título revista abreviado:Exp. Parasitol.
ISSN:00144894
CODEN:EXPAA
CAS:Antibodies, Monoclonal; Glycoproteins; Glycosylphosphatidylinositols; Membrane Proteins; Protozoan Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144894_v82_n3_p290_Abuin

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Citas:

---------- APA ----------
Abuin, G., Couto, A.S., De Lederkremer, R.M., Casal, O.L., Galli, C., Colli, W. & Alves, M.J.M. (1996) . Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor. Experimental Parasitology, 82(3), 290-297.
http://dx.doi.org/10.1006/expr.1996.0036
---------- CHICAGO ----------
Abuin, G., Couto, A.S., De Lederkremer, R.M., Casal, O.L., Galli, C., Colli, W., et al. "Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor" . Experimental Parasitology 82, no. 3 (1996) : 290-297.
http://dx.doi.org/10.1006/expr.1996.0036
---------- MLA ----------
Abuin, G., Couto, A.S., De Lederkremer, R.M., Casal, O.L., Galli, C., Colli, W., et al. "Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor" . Experimental Parasitology, vol. 82, no. 3, 1996, pp. 290-297.
http://dx.doi.org/10.1006/expr.1996.0036
---------- VANCOUVER ----------
Abuin, G., Couto, A.S., De Lederkremer, R.M., Casal, O.L., Galli, C., Colli, W., et al. Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor. Exp. Parasitol. 1996;82(3):290-297.
http://dx.doi.org/10.1006/expr.1996.0036