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Abstract:

Trypanosoma cruzi λgtll library from epimastogote derived mRNA was screened with human chagasic sera or sera from chronically infected mice. Strong reactive recombinants were detected with both sera. Two recombinant clones were studied in more detail and shown to be composed of the same 114-bp repetitive sequence coding for a 38 amino acid repetition. This repetition is the same size and shares >60% homology with the reported T. brucei microtubule associated protein (MAP) p320. The insert of one of these clones, K1-7 (228 bp), was subcloned into pMSgt11 and the soluble recombinant polypeptide expressed. Antibodies against the K1-7 fusion polypeptide recognized a major 110-kDa band from Cytoskeleton. Anti K1-7 monospecific antibodies detected several cytoskeletal proteins from 3T3 fibroblasts and bovine brain microtubule preparations. Reciprocally, anti-MAP1b monoclonal antibodies raised against bovine brain microtubule reacted with the K1-7 polypeptide on Western blots. The protein identified by K1-7 antibodies may be one of the parasite molecules associated to molecular mimicry. © 1991.

Registro:

Documento: Artículo
Título:Trypanosoma cruzi: Antibodies to a MAP-like protein in chronic chagas' disease cross-react with mammalian cytoskeleton
Autor:Kerner, N.; Liégeard, P.; Levin, M.J.; Hontebeyrie-Joskowicz, M.
Filiación:Institut Pasteur, 28, Rue du Docteur Roux, 75724 Paris Cedex15, France
Instituto de Ingeniaria Génética y Biologia Molecular, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:cDNA library; Chagas' disease; Cytoskeleton; Immunoscreening; Molecular mimicry; Protozoa; Trypanosoma cruzi MAP-like antigen; autoantibody; hybrid protein; microtubule associated protein; microtubule associated protein 5; Microtubule Associated Proteins; microtubule-associated protein 1B; parasite antigen; protozoon antibody; amino acid sequence; animal; article; Chagas disease; chronic disease; cross reaction; cytoskeleton; immunology; molecular genetics; mouse; nucleotide repeat; nucleotide sequence; Amino Acid Sequence; Animal; Antibodies, Protozoan; Antigens, Protozoan; Autoantibodies; Base Sequence; Chagas Disease; Chronic Disease; Cross Reactions; Cytoskeleton; Mice; Microtubule-Associated Proteins; Molecular Sequence Data; Recombinant Fusion Proteins; Repetitive Sequences, Nucleic Acid; Support, Non-U.S. Gov't
Año:1991
Volumen:73
Número:4
Página de inicio:451
Página de fin:459
DOI: http://dx.doi.org/10.1016/0014-4894(91)90069-9
Título revista:Experimental Parasitology
Título revista abreviado:Exp. Parasitol.
ISSN:00144894
CODEN:EXPAA
CAS:Antibodies, Protozoan; Antigens, Protozoan; Autoantibodies; microtubule-associated protein 1B; Microtubule-Associated Proteins; Recombinant Fusion Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144894_v73_n4_p451_Kerner

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Citas:

---------- APA ----------
Kerner, N., Liégeard, P., Levin, M.J. & Hontebeyrie-Joskowicz, M. (1991) . Trypanosoma cruzi: Antibodies to a MAP-like protein in chronic chagas' disease cross-react with mammalian cytoskeleton. Experimental Parasitology, 73(4), 451-459.
http://dx.doi.org/10.1016/0014-4894(91)90069-9
---------- CHICAGO ----------
Kerner, N., Liégeard, P., Levin, M.J., Hontebeyrie-Joskowicz, M. "Trypanosoma cruzi: Antibodies to a MAP-like protein in chronic chagas' disease cross-react with mammalian cytoskeleton" . Experimental Parasitology 73, no. 4 (1991) : 451-459.
http://dx.doi.org/10.1016/0014-4894(91)90069-9
---------- MLA ----------
Kerner, N., Liégeard, P., Levin, M.J., Hontebeyrie-Joskowicz, M. "Trypanosoma cruzi: Antibodies to a MAP-like protein in chronic chagas' disease cross-react with mammalian cytoskeleton" . Experimental Parasitology, vol. 73, no. 4, 1991, pp. 451-459.
http://dx.doi.org/10.1016/0014-4894(91)90069-9
---------- VANCOUVER ----------
Kerner, N., Liégeard, P., Levin, M.J., Hontebeyrie-Joskowicz, M. Trypanosoma cruzi: Antibodies to a MAP-like protein in chronic chagas' disease cross-react with mammalian cytoskeleton. Exp. Parasitol. 1991;73(4):451-459.
http://dx.doi.org/10.1016/0014-4894(91)90069-9