Abstract:
The trypanocidal activity of catechins on Trypanosoma cruzi bloodstream trypomastigotes has been previously reported. Herein, we present the effect of epigallocatechin gallate (EGCg) on parasitemia and survival in a murine model of acute Chagas' disease as well as on the epimastigote form of the parasite. Upon intraperitoneal administration of daily doses of 0.8 mg/kg/day of EGCg for 45 days, mice survival rates increased from 11% to 60%, while parasitemia diminished to 50%. No side effects were observed in EGCg-treated animals. Fifty percent inhibition of epimastigotes growth was achieved with 311 μM EGCg 120 h after drug addition. No lysis, total culture growth inhibition or morphological changes were observed upon addition of 1-3 mM EGCg at 24 h. This treatment also produced oligosomal fragmentation of epimastigotes DNA, suggesting a programmed cell death (PCD)-like process. All these findings point out EGCg as a potential new lead compound for chemotherapy of Chagas' disease. © 2007 Elsevier Inc. All rights reserved.
Registro:
Documento: |
Artículo
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Título: | Trypanosoma cruzi: In vitro and in vivo antiproliferative effects of epigallocatechin gallate (EGCg) |
Autor: | Güida, M.C.; Esteva, M.I.; Camino, A.; Flawiá, M.M.; Torres, H.N.; Paveto, C. |
Filiación: | Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Facultad de Ciencias Exactas y Naturales (CONICET-UBA), 1428 Buenos Aires, Argentina Instituto Nacional de Parasitología Dr. Mario Fatala Chabén, 1063 Buenos Aires, Argentina Unidad de hígado y transplante, Hospital Universitario Austral, Buenos Aires, Argentina
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Palabras clave: | Epigallocatechin gallate; PCD; Programmed cell death; Trypanocide Chagas' disease; epigallocatechin gallate; animal experiment; animal model; animal tissue; apoptosis; article; Chagas disease; controlled study; drug mechanism; fragmentation reaction; growth inhibition; histopathology; in vitro study; in vivo study; male; mouse; nonhuman; parasitemia; priority journal; survival rate; Trypanosoma cruzi; trypomastigote; Animals; Antioxidants; Catechin; Chagas Disease; Disease Models, Animal; DNA Fragmentation; Dose-Response Relationship, Drug; Hepatocytes; In Situ Nick-End Labeling; Male; Mice; Mice, Inbred BALB C; Parasitemia; Random Allocation; Trypanocidal Agents; Trypanosoma cruzi; Animalia; Murinae; Mus; Trypanosoma cruzi |
Año: | 2007
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Volumen: | 117
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Número: | 2
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Página de inicio: | 188
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Página de fin: | 194
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DOI: |
http://dx.doi.org/10.1016/j.exppara.2007.04.015 |
Título revista: | Experimental Parasitology
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Título revista abreviado: | Exp. Parasitol.
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ISSN: | 00144894
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CODEN: | EXPAA
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CAS: | epigallocatechin gallate, 989-51-5; Antioxidants; Catechin, 154-23-4; epigallocatechin gallate, 989-51-5; Trypanocidal Agents
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144894_v117_n2_p188_Guida |
Referencias:
- Ahmad, N., Feyes, D.K., Nieminen, A.L., Agarwal, R., Mukhtar, H., Green tea constituent epigallocatechin-3-gallate and induction of apoptosis and cell cycle arrestin in human carcinoma cells (1997) Journal of the National Cancer Institute, 89, pp. 1881-1886
- Ameisen, J.C., Idziorek, T., Billaut-Mulot, O., Loyens, M., Tissier, J.P., Potentier, A., Ouaissi, A., Apoptosis in a unicellular eukaryote (Trypanosoma cruzi): implications for the evolutionary origin and role of programmed cell death in the control of cell proliferation, differentiation and survival (1995) Cell Death and Differentiation, 2, pp. 285-300
- Asres, K., Bucar, F., Knauder, E., Yardley, V., Kendrick, H., Croft, S.L., In vitro antiprotozoal activity of extract and compounds from the stem bark of Combretum molle (2001) Phytotherapy Research, 15, pp. 6113-6117
- Bohm, B.A., Extraction, purification and identification of flavonoids (1998) Introduction to Flavonoids, pp. 175-241. , Ravindranath B. (Ed), Harwood Academic Publishers, Vancouver, British Columbia, Canada
- Coura, J.R., de Castro, S.L., A critical review on Chagas disease chemotherapy (2002) Memoria do Instituto Oswaldo Cruz, 97, pp. 3-24
- Debrabant, A., Lee, N., Bertholet, S., Duncan, R., Nakhasi, H.L., Programmed cell death in trypanosomatids and other unicellular organisms (2003) International Journal for Parasitology, 33, pp. 257-267
- Dias, J.C.P., Silveira, A.C., Schofield, C.J., The impact of Chagas'disease control in Latin America. A Review (2002) Memoria do Instituto Oswaldo Cruz, 97, pp. 607-612
- Duszenko, M., Figarella, K., Macleod, E.T., Welburn, S.C., Death of a trypanosome: a selfish altruism (2006) Trends in Parasitology, 11, pp. 536-542
- Ellman, G.L., Tissue sulfhydryl groups (1959) Archives of Biochemistry and Biophysics, 82, pp. 70-77
- Figarella, K., Rawer, M., Uzcategui, N.L., Kubata, B.K., Lauber, K., Madeo, F., Wesselborg, S., Duszenko, M., Prostaglandin D2 induces programmed cell death in Trypanosoma brucei bloodstream form (2005) Cell Death and Differentiation, 12, pp. 335-346
- Fraga, C.G., Martino, V.S., Ferraro, G.E., Coussio, J.D., Boveris, A., Flavonoids as antioxidants evaluated by in vitro and in situ liver chemiluminescence (1987) Biochemical Pharmacology, 36, pp. 717-720
- Huang, Y., Zhang, A., Lau, C.W., Chen, Z.Y.R., Vasorelaxant effects of purified green tea epigallocatechin derivatives in rat mesenteric artery (1998) Life Science, 63, pp. 275-283
- Jankun, J., Selman, S.H., Swiercz, R., Skrzypczak, E., Why drinking green tea could prevent cancer (1997) Nature, 387, p. 561
- Kayser, O., Kiderlen, A.F., Croft, S.L., Natural products as antiparasitic drugs (2003) Parasitology Research, 90, pp. 555-562
- Khan, N., Afaq, F., Saalem, M., Ahmad, N., Mukhtar, H., Targeting multiple signaling pathways by green tea polyphenol (-)-epigallocatechin-3-gallate (2006) Cancer Research, 66, pp. 2500-2505
- Kosec, G., Alvarez, V.E., Aguero, F., Sanchez, D., Dolinar, M., Turk, B., Turk, V., Cazzulo, J.J., Metacaspases of Trypanosoma cruzi: possible candidates for programmed cell death mediators (2006) Molecular and Biochemical Parasitology, 145, pp. 18-28
- Maya, J.D., Reppeto, Y., Agosin, M., Ojeda, J.M., Tellez, R., Gaule, C., Morillo, A., Effects of nifurtimox and benznidazole upon glutathione and trypanotione in epimastigote, trypomastigote, and amastigote forms of Trypanosoma cruzi (1997) Molecular and Biochemical Parasitology, 86, pp. 101-106
- Meng, X., Sang, S., Zhu, N., Lu, H., Sheng, S., Lee, M.J., Ho, C.T., Yang, C.S., Identification and characterization of methylated and ring-fission metabolites of tea catechins formed in humans, mice, and Rats (2002) Chemical Research in Toxicology, 15, pp. 1042-1050
- Moncayo, A., Ortiz Yanine, M.I., An update on Chagas disease (human American trypanosomiasis) (2006) Annals of Tropical Medicine and Parasitology, 100, pp. 663-677
- Ouaissi, A., Apoptosis-like death in trypanosomatids: search for putative pathways and genes involved (2003) Kinetoplastid Biology and Disease, 2, pp. 1-5
- Paveto, C., Güida, M.C., Esteva, M.I., Martino, V.M., Coussio, J., Flawiá, M.M., Torres, H.N., Anti-Trypanosoma cruzi activity of green tea (Camellia sinensis) catechins (2004) Antimicrobial Agents and Chemotherapy, 48, pp. 69-74
- Piacenza, L., Peluffo, G., Radi, R., l-Arginine-dependent suppression of apoptosis in Trypanosoma cruzi: Contribution of the nitric oxide and polyamine pathways (2001) Proceedings of the National Academy of Sciences of the United States of America, 98, pp. 7301-7306
- Piacenza, L., Peluffo, G., Radi, R., l-Arginine metabolism and PCD in Trypanosoma cruzi (2002) Methods in Enzymology, 359, pp. 287-302
- Sepúlveda-Boza, S., Cassels, B.K., Plant metabolites active against Trypanosoma cruzi (1996) Planta Medica, 62, pp. 98-105
- Urbina, J., Lira, R., Visbal, G., Bartroli, J., In vitro antiproliferative effect and mechanism of action of the new triazole derivative UR-9825 against the protozoan parasite Trypanosoma (Schizotrypanum) cruzi (2000) Antimicrobial Agents and Chemotherapy, 44, pp. 2498-2502
- Urbina, J.A., New chemotherapeutic approaches for the treatment of Chagas disease (American Trypanosomiasis) (2003) Expert Opinion on Therapeutic Patents, 13, pp. 661-669
- Vaux, D.I., Strasser, A., The molecular biology of apoptosis (1996) Proceedings of the National Academy of Sciences of the United States of America, 93, pp. 2239-2244
- Vijaya, K., Ananthan, S., Nalini, R., Antibacterial effect of theaflavin, polyphenon 60 (Camellia sinensis) and Euphorbia hita on Shigella spp.-a cell culture study (1995) Journal of Ethnopharmacology, 49, pp. 115-118
- Wang, X., Tian, W., Green tea epigallocatechin gallate: a natural inhibitor of fatty-acid synthase (2001) Biochemical and Biophysical Research Communication, 288, pp. 1200-1206
- Welburn, S.C., Barcinski, M.A., Williams, G.T., Programmed cell death in trypanosomatids (1997) Parasitology Today, 13, pp. 22-26
- Yamamoto, T., (1997) Chemistry and Application of Green Tea, , CRC Press, Inc., Boca Raton
- Yang, C.S., Hong, J., Hou, Z., Sang, S., Green tea poliphenols; antioxidative and prooxidative effects (2004) The Journal of Nutrition, 134, pp. 3181S
- Zhu, M., Chen, Y., Li, R.C., Oral absorption and bioavailability of tea catechins (2000) Planta Medica, 66, pp. 444-447
Citas:
---------- APA ----------
Güida, M.C., Esteva, M.I., Camino, A., Flawiá, M.M., Torres, H.N. & Paveto, C.
(2007)
. Trypanosoma cruzi: In vitro and in vivo antiproliferative effects of epigallocatechin gallate (EGCg). Experimental Parasitology, 117(2), 188-194.
http://dx.doi.org/10.1016/j.exppara.2007.04.015---------- CHICAGO ----------
Güida, M.C., Esteva, M.I., Camino, A., Flawiá, M.M., Torres, H.N., Paveto, C.
"Trypanosoma cruzi: In vitro and in vivo antiproliferative effects of epigallocatechin gallate (EGCg)"
. Experimental Parasitology 117, no. 2
(2007) : 188-194.
http://dx.doi.org/10.1016/j.exppara.2007.04.015---------- MLA ----------
Güida, M.C., Esteva, M.I., Camino, A., Flawiá, M.M., Torres, H.N., Paveto, C.
"Trypanosoma cruzi: In vitro and in vivo antiproliferative effects of epigallocatechin gallate (EGCg)"
. Experimental Parasitology, vol. 117, no. 2, 2007, pp. 188-194.
http://dx.doi.org/10.1016/j.exppara.2007.04.015---------- VANCOUVER ----------
Güida, M.C., Esteva, M.I., Camino, A., Flawiá, M.M., Torres, H.N., Paveto, C. Trypanosoma cruzi: In vitro and in vivo antiproliferative effects of epigallocatechin gallate (EGCg). Exp. Parasitol. 2007;117(2):188-194.
http://dx.doi.org/10.1016/j.exppara.2007.04.015