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The trypanocidal activity of catechins on Trypanosoma cruzi bloodstream trypomastigotes has been previously reported. Herein, we present the effect of epigallocatechin gallate (EGCg) on parasitemia and survival in a murine model of acute Chagas' disease as well as on the epimastigote form of the parasite. Upon intraperitoneal administration of daily doses of 0.8 mg/kg/day of EGCg for 45 days, mice survival rates increased from 11% to 60%, while parasitemia diminished to 50%. No side effects were observed in EGCg-treated animals. Fifty percent inhibition of epimastigotes growth was achieved with 311 μM EGCg 120 h after drug addition. No lysis, total culture growth inhibition or morphological changes were observed upon addition of 1-3 mM EGCg at 24 h. This treatment also produced oligosomal fragmentation of epimastigotes DNA, suggesting a programmed cell death (PCD)-like process. All these findings point out EGCg as a potential new lead compound for chemotherapy of Chagas' disease. © 2007 Elsevier Inc. All rights reserved.


Documento: Artículo
Título:Trypanosoma cruzi: In vitro and in vivo antiproliferative effects of epigallocatechin gallate (EGCg)
Autor:Güida, M.C.; Esteva, M.I.; Camino, A.; Flawiá, M.M.; Torres, H.N.; Paveto, C.
Filiación:Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Facultad de Ciencias Exactas y Naturales (CONICET-UBA), 1428 Buenos Aires, Argentina
Instituto Nacional de Parasitología Dr. Mario Fatala Chabén, 1063 Buenos Aires, Argentina
Unidad de hígado y transplante, Hospital Universitario Austral, Buenos Aires, Argentina
Palabras clave:Epigallocatechin gallate; PCD; Programmed cell death; Trypanocide Chagas' disease; epigallocatechin gallate; animal experiment; animal model; animal tissue; apoptosis; article; Chagas disease; controlled study; drug mechanism; fragmentation reaction; growth inhibition; histopathology; in vitro study; in vivo study; male; mouse; nonhuman; parasitemia; priority journal; survival rate; Trypanosoma cruzi; trypomastigote; Animals; Antioxidants; Catechin; Chagas Disease; Disease Models, Animal; DNA Fragmentation; Dose-Response Relationship, Drug; Hepatocytes; In Situ Nick-End Labeling; Male; Mice; Mice, Inbred BALB C; Parasitemia; Random Allocation; Trypanocidal Agents; Trypanosoma cruzi; Animalia; Murinae; Mus; Trypanosoma cruzi
Página de inicio:188
Página de fin:194
Título revista:Experimental Parasitology
Título revista abreviado:Exp. Parasitol.
CAS:epigallocatechin gallate, 989-51-5; Antioxidants; Catechin, 154-23-4; epigallocatechin gallate, 989-51-5; Trypanocidal Agents


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---------- APA ----------
Güida, M.C., Esteva, M.I., Camino, A., Flawiá, M.M., Torres, H.N. & Paveto, C. (2007) . Trypanosoma cruzi: In vitro and in vivo antiproliferative effects of epigallocatechin gallate (EGCg). Experimental Parasitology, 117(2), 188-194.
---------- CHICAGO ----------
Güida, M.C., Esteva, M.I., Camino, A., Flawiá, M.M., Torres, H.N., Paveto, C. "Trypanosoma cruzi: In vitro and in vivo antiproliferative effects of epigallocatechin gallate (EGCg)" . Experimental Parasitology 117, no. 2 (2007) : 188-194.
---------- MLA ----------
Güida, M.C., Esteva, M.I., Camino, A., Flawiá, M.M., Torres, H.N., Paveto, C. "Trypanosoma cruzi: In vitro and in vivo antiproliferative effects of epigallocatechin gallate (EGCg)" . Experimental Parasitology, vol. 117, no. 2, 2007, pp. 188-194.
---------- VANCOUVER ----------
Güida, M.C., Esteva, M.I., Camino, A., Flawiá, M.M., Torres, H.N., Paveto, C. Trypanosoma cruzi: In vitro and in vivo antiproliferative effects of epigallocatechin gallate (EGCg). Exp. Parasitol. 2007;117(2):188-194.