Artículo

Caro, F.; Bercovich, N.; Atorrasagasti, C.; Levin, M.J.; Vázquez, M.P. "Trypanosoma cruzi: Analysis of the complete PUF RNA-binding protein family" (2006) Experimental Parasitology. 113(2):112-124
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Abstract:

The members of the PUF family of RNA-binding proteins regulate the fate of mRNAs by binding to their 3′UTR sequence elements in eukaryotes. In trypanosomes, for which gene expression is polycistronic and controlled almost exclusively by post-transcriptional processes, PUF proteins could play a crucial role. We report here the complete analysis of the PUF protein family of Trypanosoma cruzi composed of 10 members. In silico analysis predicts the existence of at least three major groups within the T. cruzi family, based on their putative binding specificity. Using yeast three hybrid assays, we tested some of these predictions for TcPUF1, TcPUF3, TcPUF5, and TcPUF8 as representatives of these groups. Data mining of the T. cruzi genome led us to describe putative binding targets for the TcPUFs of the most conserved group, TcPUF1 and TcPUF2. The targets include genes for mitochondrial proteins and protein kinases. Finally, immunolocalization experiments showed that TcPUF1 is localized in multiple discrete foci in the cytoplasm supporting its proposed function. © 2005 Elsevier Inc. All rights reserved.

Registro:

Documento: Artículo
Título:Trypanosoma cruzi: Analysis of the complete PUF RNA-binding protein family
Autor:Caro, F.; Bercovich, N.; Atorrasagasti, C.; Levin, M.J.; Vázquez, M.P.
Filiación:Laboratorio de Biología Molecular de la Enfermedad de Chagas-INGEBI -CONICET, Departamento de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Vuelta de Obligado 2490 2P, 1428 Buenos Aires, Argentina
Palabras clave:aa; amino acids; Bioinformatics analysis; Chagas disease; hidden Markov model; HMM; nanos response element; NRE; open reading frame; ORF; Post-transcriptional regulation; TriTryp; Trypanosoma brucei, Trypanosoma cruzi, Leishmania major; untranslated region; UTR; Yeast three hybrids; mitochondrial protein; protein; protein kinase; protein tcpuf1; protein tcpuf3; protein tcpuf5; protein tcpuf8; RNA binding protein; unclassified drug; article; controlled study; cytoplasm; gene expression; gene sequence; genetic transcription; genomics; immunolocalization; nonhuman; nucleotide sequence; prediction; priority journal; protein analysis; protein family; RNA binding; RNA sequence; Trypanosoma cruzi; 3' Untranslated Regions; Amino Acid Sequence; Animals; Base Sequence; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Open Reading Frames; Protozoan Proteins; Repetitive Sequences, Amino Acid; RNA-Binding Proteins; Trypanosoma cruzi; Eukaryota; Leishmania major; Trypanosoma brucei; Trypanosoma cruzi
Año:2006
Volumen:113
Número:2
Página de inicio:112
Página de fin:124
DOI: http://dx.doi.org/10.1016/j.exppara.2005.12.015
Título revista:Experimental Parasitology
Título revista abreviado:Exp. Parasitol.
ISSN:00144894
CODEN:EXPAA
CAS:protein kinase, 9026-43-1; protein, 67254-75-5; 3' Untranslated Regions; Protozoan Proteins; RNA-Binding Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144894_v113_n2_p112_Caro

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Citas:

---------- APA ----------
Caro, F., Bercovich, N., Atorrasagasti, C., Levin, M.J. & Vázquez, M.P. (2006) . Trypanosoma cruzi: Analysis of the complete PUF RNA-binding protein family. Experimental Parasitology, 113(2), 112-124.
http://dx.doi.org/10.1016/j.exppara.2005.12.015
---------- CHICAGO ----------
Caro, F., Bercovich, N., Atorrasagasti, C., Levin, M.J., Vázquez, M.P. "Trypanosoma cruzi: Analysis of the complete PUF RNA-binding protein family" . Experimental Parasitology 113, no. 2 (2006) : 112-124.
http://dx.doi.org/10.1016/j.exppara.2005.12.015
---------- MLA ----------
Caro, F., Bercovich, N., Atorrasagasti, C., Levin, M.J., Vázquez, M.P. "Trypanosoma cruzi: Analysis of the complete PUF RNA-binding protein family" . Experimental Parasitology, vol. 113, no. 2, 2006, pp. 112-124.
http://dx.doi.org/10.1016/j.exppara.2005.12.015
---------- VANCOUVER ----------
Caro, F., Bercovich, N., Atorrasagasti, C., Levin, M.J., Vázquez, M.P. Trypanosoma cruzi: Analysis of the complete PUF RNA-binding protein family. Exp. Parasitol. 2006;113(2):112-124.
http://dx.doi.org/10.1016/j.exppara.2005.12.015